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[{"key": "dc.contributor.advisor", "value": "Kivel\u00e4, Riikka", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Marjom\u00e4ki, Varpu", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Gynther, Veera", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-12-23T10:48:11Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-12-23T10:48:11Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/99202", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Rabdomyosarkooma (RMS) on lasten yleisin pehmytkudossy\u00f6p\u00e4 ja muistuttaa histologialtaan erilaistuvaa luustolihasta. RMS-potilaiden ennuste on huono, ja nykyiset hoitomenetelm\u00e4t aiheuttavat vakavia sivuvaikutuksia. Prospero-related homeobox 1 (PROX1) -transkriptiofaktorin on huomattu olevan yhteydess\u00e4 RMS-solujen kasvuun, myogeenisiin ominaisuuksiin ja mitokondriogeenien s\u00e4\u00e4telyyn. PROX1-hiljennyksen on huomattu v\u00e4hent\u00e4v\u00e4n RMS-kasvainten kasvua. T\u00e4m\u00e4n tutkimuksen tavoitteena oli selvitt\u00e4\u00e4, s\u00e4\u00e4teleek\u00f6 PROX1 RMS-solujen mitkondrioverkostoa ja mitokondriaalista fissiota ja fuusiota. Kokeet tehtiin kahdella RMS-solulinjalla, RD ja KLHEL1, k\u00e4ytt\u00e4en kontrollisoluina terveit\u00e4 prim\u00e4\u00e4risi\u00e4 myoblasteja. PROX1-hiljennys ja kontrollik\u00e4sittely tehtiin hy\u00f6dynt\u00e4en lentivirusvektoria. Mitokondriot visualisoitiin MitoTracker-v\u00e4rj\u00e4yksell\u00e4 ja plasmiditransfektiolla, ja kuvantaminen suoritettiin fiksatuille ja el\u00e4ville soluille konfokaalimikroskoopilla. K\u00e4sitellyt solut segmentoitiin MitoTrackerill\u00e4 v\u00e4rj\u00e4ttyjen fiksattujen solujen kuvista Cellpose-algoritmin avulla ja niiden mitokondriot analysoitiin ImageJ-kuvank\u00e4sittelyohjelmalla. T-testi tai ei-parametrinen Mann-Whitney U -testi suoritettiin GraphPad Prism -tilasto-ohjelmalla mitokondriaalisille muuttujille vertaamalla PROX1-hiljennettyj\u00e4 ja kontrollisoluja toisiinsa. PROX1-hiljennyksen huomattiin vaikuttavan kaikkiin solutyyppeihin, mutta RD- ja KLHEL1-sy\u00f6p\u00e4soluihin hiljennys vaikutti enemm\u00e4n kuin terveisiin prim\u00e4\u00e4risiin myoblasteihin. RD- ja KLHEL1-solujen mitokondriot olivat pienempi\u00e4 ja py\u00f6re\u00e4mpi\u00e4 PROX1-hiljennyksen my\u00f6t\u00e4, mik\u00e4 viittaa mitokondriaalisen dynamiikan muutoksesta lis\u00e4\u00e4ntyneen fission ja v\u00e4hentyneen fuusion suuntaan. PROX1-hiljennys v\u00e4hensi mitokondrioita KLHEL1-soluissa, mutta ei vaikuttanut RD-solujen mitokondrioiden m\u00e4\u00e4r\u00e4\u00e4n. Terveiden prim\u00e4\u00e4risten myoblastien mitokondrioita oli v\u00e4hemm\u00e4n, mutta niiden koko tai muoto ei muuttunut PROX1-hiljennyksen my\u00f6t\u00e4. N\u00e4it\u00e4 tuloksia voidaan toivottavasti hy\u00f6dynt\u00e4\u00e4 uusien hoitomenetelmien kehittelyss\u00e4, mahdollisesti mitokondriaalista fissiota tehostamalla tai fuusiota inhiboimalla l\u00e4\u00e4kkeiden avulla.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children, and its histology resembles developing skeletal muscle. The prognosis for RMS patients is poor, and current treatment methods cause severe side effects. Prospero-related homeobox 1 (PROX1) transcription factor has been linked to growth, myogenic properties and mitochondrial gene regulation of RMS cells. PROX1 silencing has been noticed to reduce RMS tumor growth. This study aimed to determine if PROX1 regulates the mitochondrial network and mitochondrial fission and fusion in RMS cells. The experiments were conducted with two RMS cell lines, RD and KLHEL1, using healthy primary myoblasts as control cells. PROX1 silencing and control treatment were done utilising a lentiviral vector. Mitochondria were visualised with MitoTracker staining and plasmid transfection, and imaging was conducted for fixed and live cells using confocal microscopy. The treated cells were segmented from the MitoTracker stained fixed cell images with Cellpose and their mitochondria were analysed in ImageJ with a custom-made macro. A t-test or a non-parametric Mann-Whitney U test was conducted in GraphPad Prism for mitochondrial variables comparing PROX1 silenced and control cells. PROX1 silencing was noticed to affect the mitochondrial network of all cell types, but RD and KLHEL1 RMS cells were more affected than the healthy primary myoblasts. The mitochondria of RD and KLHEL1 cells were smaller and rounder due to PROX1 silencing, which indicates that mitochondrial dynamics shift towards increased mitochondrial fission and decreased mitochondrial fusion. PROX1 silencing did not affect mitochondria count in RD cells but reduced mitochondria count in KLHEL1 cells. Healthy primary myoblasts had fewer mitochondria, but their size and shape remained unchanged due to PROX1 silencing. These results can hopefully be utilised in the development of novel treatment methods for RMS, possibly through drugs enhancing mitochondrial fission or inhibiting mitochondrial fusion.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2024-12-23T10:48:11Z\r\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-12-23T10:48:11Z (GMT). No. of bitstreams: 0\r\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "57", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "alveolar rhabdomyosarcoma", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "embryonal rhabdomyosarcoma", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "fusion gene", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mesenchymal stem cell", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mitochondrial fission", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mitochondrial fusion", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mitochondrial network", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "myofiber", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "myofiber", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "oxidative phosphorylation", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "prospero-related homeobox 1", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "PROX1", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "regulation", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "rhabdomyosarcoma", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "RMS", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "satellite cell", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "skeletal muscle", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "soft tissue sarcoma", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The regulation of mitochondrial network in rhabdomyosarcoma by prospero-related homeobox 1", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202412238005", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": null, "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mitokondriot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solubiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mitokondrio-DNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kantasolut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "visualisointi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geenit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "soluviljely", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mitochondria", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell biology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mitochondrial DNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "stem cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "visualisation", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "genes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell culture", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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