| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Oja, Terhi", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Louhelainen, Jarmo", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Pappinen, Sari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Hiidenhovi, Sara", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-06-27T09:40:57Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-06-27T09:40:57Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2022", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/82040", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Useimmat uudet l\u00e4\u00e4keaineet ovat heikosti liukenevia ja l\u00e4\u00e4keaineen m\u00e4\u00e4r\u00e4 l\u00e4\u00e4kekehityksess\u00e4 on hyvin rajallinen. Sen vuoksi on tarve l\u00f6yt\u00e4\u00e4 uusia liukoisuutta parantavia formulaatioita ja pienen mittakaavan metodeja ja malleja niiden tutkimiseen. L\u00e4\u00e4kekehitys on hy\u00f6dynt\u00e4nyt el\u00e4inkokeita l\u00e4\u00e4keaineiden in vivo -k\u00e4ytt\u00e4ytymisen tutkimiseen j\u00e4rjestelem\u00e4ll\u00e4 eri formulaatioita paremmuusj\u00e4rjestykseen perustuen niiden in vivo -olosuhteissa liuottamaan l\u00e4\u00e4keaineen m\u00e4\u00e4r\u00e4\u00e4n. N\u00e4it\u00e4 j\u00e4rjestyksi\u00e4 voidaan verrata in vitro -tuloksiin, jotka saadaan pienen mittakavaan dissoluutio-permeaatiomallin mittauksista.\n\nTutkimuksen tavoitteena oli tutkia kokeellisten olosuhteiden vaikutusta pienen mittakavaan dissoluutio-permeaatiomallin toimivuuteen l\u00e4\u00e4kekehityksess\u00e4. Lis\u00e4ksi haluttiin tutkia kykeneek\u00f6 tutkittava in vitro -malli tehokkaasti mallintamaan rotan in vivo suolisto-olosuhteita. Tutkittavat kokeelliset olosuhteet olivat k\u00e4ytett\u00e4v\u00e4n annoksen ja v\u00e4liaineen vaikutukset. Tutkimukset suoritettiin nelj\u00e4ll\u00e4 eri liukoisuutta parantavalla formulaatiosuspensiolla. Tavoitteena oli selvitt\u00e4\u00e4 tuottaako k\u00e4ytetty pienen mittakaavan malli saman j\u00e4rjestyksen formulaatiosuspensioiden v\u00e4lill\u00e4 kuin aiemmissa rotilla suoritetuissa mittauksissa oltiin havaittu. Lis\u00e4ksi tutkittiin in vitro ja in vivo -tulosten v\u00e4list\u00e4 korrelaatiota.\n\nTuloksista n\u00e4htiin eroja kokeellisten olosuhteiden v\u00e4lill\u00e4. Erityisesti k\u00e4ytetyn v\u00e4liaineen huomattiin aiheuttavan muuntelua tulosten v\u00e4lill\u00e4. Tuloksista havaittiin, ett\u00e4 annoksella ei ollut yksin\u00e4\u00e4n suurta vaikutusta, mutta k\u00e4ytetty v\u00e4liaine lis\u00e4si my\u00f6s annoksen vaikutusta. Voitiin todeta, ett\u00e4 fosfaattipuskuri pH 3.0 v\u00e4liaineessa tehdyt mittaukset tuottivat tasaisempia ja t\u00e4sm\u00e4llisempi\u00e4 tuloksia jopa alemmalla annoksella. Sen sijaan FeSSIF v\u00e4liaineessa korkeampi annos oli suositeltavampi. Tulosten perusteella FeSSIF v\u00e4liaineen koostumusta ja siten sen vaikutusta dissoluutio-permeaatiomalliin tulisi tutkia viel\u00e4 lis\u00e4\u00e4.\n\nNelj\u00e4n eri suspensioformulaatioiden v\u00e4lill\u00e4 saatiin sama j\u00e4rjestys molemmilla v\u00e4liaineilla ja annosm\u00e4\u00e4rill\u00e4. N\u00e4m\u00e4 olivat samat kuin aiemmissa in vivo -testeiss\u00e4 havaitut j\u00e4rjestykset. Tutkimuksissa saatiin my\u00f6s suhteellisen korkeita korrelaatioita in vitro ja in vivo -tulosten v\u00e4lill\u00e4. T\u00e4ten tutkittu dissoluutio-permeaatiomalli soveltuu mallintamaan rotan suolisto-olosuhteita ja sit\u00e4 voidaan tulevaisuudessa k\u00e4ytt\u00e4\u00e4 t\u00e4m\u00e4n l\u00e4\u00e4keaineen pienen mittakaavan dissoluutio-permeaatiotutkimuksissa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Most of the newly-developed drugs are poorly-soluble and the amount of drug substance available for drug development is very limited. Therefore, there is a need to find new solubility-enhancing formulations and small-scale methods and models to study them. Drug development has utilized animal models to study drug substances\u2019 in vivo behavior, by ranking the formulations according to the dissolved drug substance amounts with in vivo conditions. These rankings can be compared to in vitro results, which are obtained from small-scale dissolution-permeation model\u2019s measurements.\n\nThe aim of this study was to investigate what effect experimental conditions have on a small-scale dissolution-permeation model in drug development and if the studied in vitro model could efficiently simulate rat\u2019s gastrointestinal in vivo conditions. Studied experimental conditions were dose effect and used medium\u2019s effect. Experiments were performed with four different solubility enhancing suspension formulations. The aim was to find if used small-scale model was able to produce same ranking between suspension formulations as was previously obtained with rat studies. It was also investigated if correlation could be obtained between in vitro and in vivo results.\n\nThe results indicated differences between experimental conditions. Especially used medium caused variation between results. From the results was seen that dose itself did not have a major effect, but used medium also enhanced dose\u2019s impact. Results showed that measurements made with phosphate buffer pH 3.0 were able to produce constant and accurate results even with lower dose. With FeSSIF, higher dose was preferable, and the medium composition and thus its performance in dissolution-permeation studies needs more research.\n\nThe same ranking between four suspension formulations were obtained with both media and both doses. Rankings were same as in vivo tests have been obtained. In the studies were also obtained relatively high correlations between in vitro and in vivo results. Due to correct suspension formulation rankings and good correlation between in vitro and in vivo results, it could be concluded that studied dissolution-permeation model could simulate rat\u2019s gastrointestinal conditions. Therefore it could be used in the future, with this drug substance, for small-scale dissolution-permeation studies.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2022-06-27T09:40:57Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-06-27T09:40:57Z (GMT). No. of bitstreams: 0\n Previous issue date: 2022", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "100", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "dissolution-permeation", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MicroFLUX", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "small-scale", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "poorly-soluble", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "drug development", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "in vivo", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "in vitro", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "gastrointestinal tract", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "biorelevant", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "biorelevant media", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "bioequivalance", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "permeability", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "flux", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "dissolution", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "solubility", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "permeation", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "biopharmaceutical", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "biopharmaceutical classification", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "suspension formulations", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The effect of experimental conditions on the performance of a small-scale dissolution-permeation model in drug development", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202206273644", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Kemian laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Chemistry", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Analyyttinen kemia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Analytical Chemistry", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.collaborator", "value": "business", "language": "", "element": "contractresearch", "qualifier": "collaborator", "schema": "yvv"}, {"key": "yvv.contractresearch.funding", "value": "n. 1600", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "yvv.contractresearch.initiative", "value": "business", "language": "", "element": "contractresearch", "qualifier": "initiative", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "40311", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "farmakokinetiikka", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "imeytyminen", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "l\u00e4\u00e4keteollisuus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "pharmacokinetics", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "absorption", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "pharmaceutical industry", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|