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[{"key": "dc.contributor.advisor", "value": "Salminen, Sami", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Vihinen-Ranta, Maija", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Lahtinen, Nuutti", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Koivisto, Ida", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Vartiainen, Jennika", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-01-24T06:17:53Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-01-24T06:17:53Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/79468", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Tyypin 1 Herpes Simplex \u2013virus on maailmanlaajuisesti levinnyt yleinen virus. Se \nlevi\u00e4\u00e4 yleisimmin limakalvokontaktissa ja voi aiheuttaa huuliherpeksen. Virus \naiheuttaa lyyttisen infektion epiteelisoluissa. Infektio voi my\u00f6hemmin levit\u00e4 my\u00f6s \naluetta hermottaviin hermosoluihin, joissa se aiheuttaa latentin infektion. Virus \nreplikoituu is\u00e4nt\u00e4solun tumassa, jossa tapahtuu my\u00f6s uusien kapsidien \nkokoaminen. Is\u00e4nt\u00e4solun perim\u00e4 on pakattu tumaan pieneen tilavuuteen \nkromatiiniksi, jonka rakenne vaikuttaa siihen, miten viruskapsidit p\u00e4\u00e4sev\u00e4t \nliikkumaan tumassa. Herpesinfektio aiheuttaa is\u00e4nt\u00e4solun tuman rakenteissa\nmuutoksia, joihin kuuluu replikaatioalueiden laajeneminen, kromatiinin \npakkautuminen tuman reunoille sek\u00e4 tuman koon kasvaminen. Tumassa kapsidit \nliikkuvat diffuusion avulla p\u00e4\u00e4asiassa kromatiinin v\u00e4litiloissa ja poistuvat \nkuroutumalla sisemp\u00e4\u00e4n tumakalvoon. T\u00e4ss\u00e4 tutkielmassa pyrimme selvitt\u00e4m\u00e4\u00e4n, \nvaikuttaako is\u00e4nt\u00e4solun tuman kromatiinin rakenteen muuntelu siihen, miten \ntyypin 1 Herpes Simplex -viruskapsidit sijoittuvat tumassa. T\u00e4m\u00e4n selvitt\u00e4miseksi \nVero-solulinjan solujen kromatiinia kondensoitiin tai dekondensoitiin, ja solut \ninfektoitiin viruksilla. N\u00e4ytesolut immunoleimattiin ja kuvattiin \nkonfokaalimikroskoopilla. Kuvista havaittiin, ett\u00e4 viruskapsidit p\u00e4\u00e4siv\u00e4t \ndekondensoitujen solujen kromatiinin sekaan paremmin kuin kondensoitujen. \nKuitenkaan kapsidijakaumissa ei n\u00e4kynyt merkitt\u00e4v\u00e4\u00e4 eroa. Kapsidijakauma oli \nsamankaltainen l\u00e4hes koko mittausalueella, mik\u00e4 poikkesi odotetusta.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Type 1 Herpes Simplex virus (HSV-1) is a common globally spread virus. It is \ntransmitted most commonly by direct mucous membrane contact and it can be a \ncause of cold sores. The virus causes a lytic infection in epithelial cells. The infection \ncan later spread to nerve cells innervating the area where it causes a latent infection. \nReplication of the virus takes place inside the nucleus of a host cell where also \nprogeny capsids are assembled. The genome of a host cell is packed in the nucleus \ninto a smaller volume as chromatin and its structure affects how the capsids move \nin the nucleus. Herpes infection leads to structural changes in the host cell\u2019s nucleus \nincluding chromatin marginalization to nucleus\u2019s periphery and expansion of the \nnucleus and viral replication compartment. In the nucleus capsids move by \ndiffusion mainly in interchromatin spaces and egress by budding into the inner \nnuclear membrane. In this study our goal was to examine if the changes in host cell\u2019s \nchromatin structure affects the localization of the type 1 Herpes Simplex virus \ncapsids in the nucleus. This was studied by condensing or decondensing the \nchromatin structure of infected Vero cells. Sample cells were immunolabeled and \nimaged using confocal microscopy. Image data analyses showed that the viral \ncapsids had better access into the chromatin in the decondensed cells than in the \ncondensed cells. However, no significant difference was seen in the capsid \ndistributions. The capsid distribution was similar over almost the entire measuring \nrange which differed from the expected.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2022-01-24T06:17:53Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-01-24T06:17:53Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "25", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "kromatiinin kondensaatio", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "kromatiinin dekondensaatio", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "lyyttinen infektio", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "tyypin 1 Herpes Simplex- virus", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Kromatiinin rakennemuutosten vaikutus HSV-1 \u2013 kapsidien sijaintiin tumassa", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "bachelor thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202201241244", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Bachelor's thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Kandidaatinty\u00f6", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_7a1f", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "bachelorThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "tuma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virustaudit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infektiot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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