Microbiota and reduction of volatile organic compounds in commercial green wall systems

Sisäilman laadun ylläpidossa pääpainona on ollut haihtuvien orgaanisten yhdisteiden (VOC-yhdisteiden) vähentäminen. Kaupungistuminen on vähentänyt ihmisten altistumista ympäristömikrobeille, jonka on havaittu vaikuttavan immuunisairauksien yleistymiseen. Aktiiviviherseinät hyödyntävät monimuotoista...

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Main Author: Salmivirta, Elisa
Other Authors: Matemaattis-luonnontieteellinen tiedekunta, Faculty of Sciences, Bio- ja ympäristötieteiden laitos, Department of Biological and Environmental Science, Jyväskylän yliopisto, University of Jyväskylä
Format: Master's thesis
Language:eng
Published: 2020
Subjects:
Online Access: https://jyx.jyu.fi/handle/123456789/73451
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author Salmivirta, Elisa
author2 Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_facet Salmivirta, Elisa Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä Salmivirta, Elisa Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_sort Salmivirta, Elisa
datasource_str_mv jyx
description Sisäilman laadun ylläpidossa pääpainona on ollut haihtuvien orgaanisten yhdisteiden (VOC-yhdisteiden) vähentäminen. Kaupungistuminen on vähentänyt ihmisten altistumista ympäristömikrobeille, jonka on havaittu vaikuttavan immuunisairauksien yleistymiseen. Aktiiviviherseinät hyödyntävät monimuotoista mikrobistoa VOC-yhdisteiden hajottamiseen. VOC-yhdisteiden poistotehosta in situ ei ole julkaistu ennen tätä tutkimusta ja aiemmat mikrobiyhteisötutkimukset ovat keskittyneet VOC-hajottajamikrobien tunnistamiseen. Poistotehoa seurattiin keräämällä VOC-näytteitä kolmesta eri mittauspisteestä—viherseinän ulostuloilmasta, viherseinän sisältämästä huoneesta, sekä kontrollihuoneesta ilman viherseinää. Mikrobiyhteisöjen rakennetta tutkittiin 16S rRNA sekvensoimalla näytteitä hanavedestä, viherseinän kasteluvedestä, sekä viherseinän ulostuloilmasta. VOC-yhdisteiden kokonaispitoisuudet havaittiin mataliksi, eikä mittauspisteiden välillä havaittu merkitseviä eroja. Kasteluveden mikrobiyhteisön havaittiin olevan Betaproteobacteriales –heimon dominoima. Kaksiulotteisessa NMDS-ordinaatio kuvaajassa aktiiviviherseinän ulostuloilman mikrobiomi erottui omaksi ryhmäkseen vesinäytteistä, ja muistutti sisäilman tyypillistä mikrobiomia, sisältäen runsaasti iholla esiintyviä aktinobakteereja. Lajispesifisellä qPCR –menetelmällä testattu patogeeninen Legionella pneumophila ei huomattu lisääntyvän aktiiviviherseinissä, vaikka patogeeni havaittiin muutamista näytteistä. The reduction of volatile organic compounds (VOCs) from the indoor air has been the priority against bad indoor air quality. Additionally, urbanization has reduced human exposure to environmental microbes, which might have led to the growing popularity of inflammatory diseases. An active green wall employs a rich microbial community with VOC degradation potential for indoor air treatment. Before this study, in situ testing of VOC-removal abilities has been limited and microbial community studies have mainly focused on taxa with VOC-degrading potential. We studied VOC removal abilities by collecting indoor air in three different points—green wall outlet fan, room with a green wall and control room without the green wall. High-throughput sequencing of 16S rRNA was utilized to screen microbe communities in tap and irrigation tank water, and air collected from the green wall outlet fan. A species-specific qPCR was applied to detect and quantify pathogenic L. pneumophila .Total VOC concentrations were generally low and no significant differences between VOC collection points were found. Irrigation tank water had high abundance of order Betaproteobacteriales, likely derived from tap water. Abundance of emitted bacteria was found moderate and air community structure resembled the normal indoor air microbiota with skin-related Actinobacteria dominating the microbial diversity. The presence of L. pneumophila was recognized, yet the pathogen was found not to proliferate inside the green wall systems.
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spellingShingle Salmivirta, Elisa Microbiota and reduction of volatile organic compounds in commercial green wall systems next-generation sequencing microbial community Solu- ja molekyylibiologia Cell and molecular biology 4013 viherseinät sisäilma haihtuvat orgaaniset yhdisteet ilmanlaatu green walls indoor air volatile organic compounds air quality
title Microbiota and reduction of volatile organic compounds in commercial green wall systems
title_full Microbiota and reduction of volatile organic compounds in commercial green wall systems
title_fullStr Microbiota and reduction of volatile organic compounds in commercial green wall systems Microbiota and reduction of volatile organic compounds in commercial green wall systems
title_full_unstemmed Microbiota and reduction of volatile organic compounds in commercial green wall systems Microbiota and reduction of volatile organic compounds in commercial green wall systems
title_short Microbiota and reduction of volatile organic compounds in commercial green wall systems
title_sort microbiota and reduction of volatile organic compounds in commercial green wall systems
title_txtP Microbiota and reduction of volatile organic compounds in commercial green wall systems
topic next-generation sequencing microbial community Solu- ja molekyylibiologia Cell and molecular biology 4013 viherseinät sisäilma haihtuvat orgaaniset yhdisteet ilmanlaatu green walls indoor air volatile organic compounds air quality
topic_facet 4013 Cell and molecular biology Solu- ja molekyylibiologia air quality green walls haihtuvat orgaaniset yhdisteet ilmanlaatu indoor air microbial community next-generation sequencing sisäilma viherseinät volatile organic compounds
url https://jyx.jyu.fi/handle/123456789/73451 http://www.urn.fi/URN:NBN:fi:jyu-202012287383
work_keys_str_mv AT salmivirtaelisa microbiotaandreductionofvolatileorganiccompoundsincommercialgreenwallsystems