| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Sundberg, Lotta-Riina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Kunttu, Heidi", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Runtuvuori-Salmela, Anniina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Sorsa, Joanda", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2020-06-25T10:58:44Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2020-06-25T10:58:44Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2020", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/70928", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Flavobacterium columnare on Gram-negatiivinen bakteeri, joka aiheuttaa columnaris-tautia. Columnaris-tauti aiheuttaa taloudellista menetyst\u00e4 kalanviljelylaitoksilla, ja tauti on vuosikymmenten aikana yleistynyt. Taudin hoitokeinona on p\u00e4\u00e4asiallisesti k\u00e4ytetty antibiootteja, mutta taudin ehk\u00e4isyss\u00e4 ja hoidossa voidaan mahdollisesti hy\u00f6dynt\u00e4\u00e4 my\u00f6s bakteriofageja. T\u00e4m\u00e4n tutkimuksen tavoitteena oli selvitt\u00e4\u00e4 (1) miten faagit vaikuttavat bakteerin taudinaiheuttamiskykyyn liittyv\u00e4\u00e4n pes\u00e4kemorfologiaan ja faagiresistenssin kehittymiseen, (2) voidaanko bakteeria infektoida yht\u00e4 faagia tehokkaammin faagikoktaililla, joka sis\u00e4lt\u00e4\u00e4 yht\u00e4 tai kahta faagityyppi\u00e4 ja (3) voidaanko faageja evolvoida infektoimaan faagiresistenttej\u00e4 bakteeri-isolaatteja. T\u00e4ss\u00e4 ty\u00f6ss\u00e4 eri puolilta Suomea sijaitsevilta kalanviljelylaitoksilta eristettyj\u00e4 F. columnare -bakteerikantoja altistettiin joko yhdelle tai kahdelle niit\u00e4 infektoivalle faagille. K\u00e4sittelyist\u00e4 m\u00e4\u00e4ritettiin bakteeritiheys, pes\u00e4kkeiden morfologiajakauma ja faagitiitteri. Kokeen lopussa seulottiin evolvoituneita faageja. Tuloksiksi saatiin, ett\u00e4 l\u00e4hes kaikissa k\u00e4sittelyiss\u00e4 pes\u00e4kkeiden morfologia muuttui, mik\u00e4 saattaa viitata taudinaiheuttamiskykyisempien bakteerien m\u00e4\u00e4r\u00e4n v\u00e4henemiseen. Faagiresistenttiys saattoi usein vaikuttaa bakteerin pintareseptoriin. Kahden faagin k\u00e4ytt\u00e4minen yhden sijasta ei voimistanut bakteerin infektointia. Kahta faagityyppi\u00e4 sis\u00e4lt\u00e4v\u00e4ss\u00e4 k\u00e4sittelyss\u00e4 havaittiin evolvoituneita faageja. Tulokset tukevat faagien mahdollista hy\u00f6dynt\u00e4mist\u00e4 columnaris-tautia vastaan.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Flavobacterium columnare is a Gram-negative bacterium causing columnaris disease. This disease causes economical losses at fish farms and during the last decades the number of outbreaks has increased. The main cure has been antibiotics, but also bacteriophages could be used. The research questions of the study were (1) how presence of phages change bacteria\u2019s colony morphology related to virulence, and development of phage resistance, (2) does phage cocktail of one or two phage types infect bacterium more effectively than one phage, and (3) is it possible to evolve phages to infect phage-resistant bacterial isolates. Bacteria and their phages used in this study were previously isolated from Finnish fish farms. F. columnare bacterium strain was exposed to either one or two phages. From the treatments, bacterium concentration, distribution of bacterium colony morphotype and phage titre were determined. At the end of the experiment, phages which could infect phage resistant bacterium were screened. As a result, almost in all treatments bacterium morphology changed. This might suggest that the number of more virulent bacterium decreased. Phage resistance might often affect the surface receptor of bacterium. Use of two phages was not more effective than one for infecting bacterium. Evolved phages from two phage type treatment were isolated. Results support the scenario of utilizing phages against columnaris disease.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2020-06-25T10:58:44Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2020-06-25T10:58:44Z (GMT). No. of bitstreams: 0\n Previous issue date: 2020", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "59", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "columnaris-tauti", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "evolvoitunut faagi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "faagiresistentti", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Faagikoktailien vaikutus Flavobacterium columnare -bakteeriin ja faagien evoluutio", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202006255116", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteriofagit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteeritaudit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|