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[{"key": "dc.contributor.advisor", "value": "Sundberg, Lotta-Riina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Laanto, Elina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "M\u00e4ntynen, Sari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "M\u00e4kel\u00e4, Kati", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2019-03-04T07:19:21Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2019-03-04T07:19:21Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2019", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/63001", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Yksijuosteisia DNA (ssDNA) -faageja sek\u00e4 lipidikalvollisia ja h\u00e4nn\u00e4tt\u00f6mi\u00e4 faageja on eristetty ja tutkittu suhteellisen v\u00e4h\u00e4n, vaikka ne ovat huomattavasti yleisempi\u00e4 ja ekologisesti merkitt\u00e4v\u00e4mpi\u00e4, kuin aiemmin on oletettu. FLiP on Jyv\u00e4sj\u00e4rvest\u00e4 vuonna 2010 eristetty flavobakteereja infektoiva ssDNA-faagi, jolla on lipidikalvo ikosahedraalisesti symmetrisen, h\u00e4nn\u00e4tt\u00f6m\u00e4n proteiinikapsidin sis\u00e4puolella. T\u00e4m\u00e4n tutkimuksen tavoitteena oli analysoida FLiP:n ja sen is\u00e4nt\u00e4bakteerien v\u00e4lisi\u00e4 vuorovaikutuksia. FLiP:n elinkiertoa tarkasteltiin adsorptiotestien ja kasvuk\u00e4yr\u00e4kokeiden avulla ja sen proteiineja analysoitiin kemikaalik\u00e4sittelyjen, elektroforeesin ja zymografian avulla. Is\u00e4nt\u00e4kirjon evoluutiokokeissa FLiP-faagia lis\u00e4ttiin eristysis\u00e4nn\u00e4st\u00e4 ja mahdollisista uusista is\u00e4nnist\u00e4 koostuviin sekaviljelmiin. Adsorptiotestien nesteviljelmiss\u00e4 FLiP kiinnittyi ainoastaan eristysis\u00e4nt\u00e4\u00e4ns\u00e4, vaikka puolikiinteill\u00e4 kasvatusalustoilla FLiP kykenee infektoimaan lis\u00e4ksi my\u00f6s kahta muuta bakteerikantaa. FLiP:n proteiineista saatiin uutta tietoa. FLiP:ll\u00e4 esimerkiksi havaittiin useita lyyttisi\u00e4 entsyymej\u00e4. T\u00e4m\u00e4 saattaisi viitata endolyysiinien esiintymiseen virionissa sek\u00e4 mahdollisesti my\u00f6s faagin laajaan is\u00e4nt\u00e4kirjoon. Mik\u00e4li FLiP:n is\u00e4nt\u00e4kirjo laajenisi kaloille patogeenisiin flavobakteerikantoihin, FLiP voisi toimia hyv\u00e4n\u00e4 faagiterapian v\u00e4lineen\u00e4 tappaessaan kohdennetusti ja tehokkaasti kalojen kudoksiin kiinnittyneit\u00e4 bakteereja.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Single-stranded DNA (ssDNA) phages, lipid-containing phages and non-tailed phages are more common and ecologically more significant than previously considered, though still relatively scarcely isolated and researched. FLiP is a Flavobacterium-infecting virus, which has been isolated from the lake Jyv\u00e4sj\u00e4rvi in 2010. FLiP has an internal lipid membrane, ssDNA genome and icosahedral, nontailed capsid. The aim of this study was to analyze interactions between FLiP and its host bacteria. Adsorption tests and one-step cultivations were performed to analyse adsorption and infective cycle of FLiP. Chemical treatments, electrophoresis and zymography were used to analyse phage proteins. Mixed cultures of original and potential new hosts were applied to test host-range evolution of FLiP. FLiP can infect three bacterial strains on semi-solid agar, but it could only adsorb onto its original host in liquid cultivations of adsorption tests. New information on FLiP proteins was acquired; for example, several lytic enzymes were observed. That might suggest that FLiP contains endolysins. Several lytic enzymes might also implicate that the phage has broad host range. If the host range of FLiP was broadened to fish-pathogenic Flavobacterium-strains, FLiP could be used as a good agent for phage therapy targeting specifically and effectively the bacteria, which are attached to fish tissues.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2019-03-04T07:19:21Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2019-03-04T07:19:21Z (GMT). No. of bitstreams: 0\n Previous issue date: 2019", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "54", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "endolysiinit", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "faagi-bakteerivuorovaikutukset", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Flavobakteereja infektoivan ssDNA-faagi FLiP:n is\u00e4nt\u00e4vuorovaikutukset", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201903041704", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteriofagit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "elinkierto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "molekyylibiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "entsyymit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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