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[{"key": "dc.contributor.advisor", "value": "Toppari, Jussi", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Lehtivuori, Heli", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Nuuttila, Lauri", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2017-09-15T09:13:14Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2017-09-15T09:13:14Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2017", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1723356", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/55371", "language": "", "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Poikkitieteelliset sovellukset ovat viime aikoina yleistyneet tieteellisess\u00e4 tutkimuksessa. T\u00e4m\u00e4 n\u00e4kyy hyvin esimerkiksi el\u00e4v\u00e4n kudoksen kuvantamisen kehittymisess\u00e4, jota varten etsit\u00e4\u00e4n jatkuvasti parempia biologisia leimoja fysiikan ja kemian keinoin. T\u00e4ss\u00e4 tutkielmassa on kehitetty ensimm\u00e4inen l\u00e4hi-infrapuna-alueen fluoresenssileima, joka hy\u00f6dynt\u00e4\u00e4 hopeananopartikkeleja ja fytokromeja. Kompleksi on syntetisoitu itse ja sen rakenne on todennettu optisen spektroskopian, elektroforeesin ja elektronimikroskopian keinoin. Lupaavat fluoresenssiominaisuudet saatiin k\u00e4ytt\u00e4m\u00e4ll\u00e4 monomeerisen Deinococcus radiodurans -fytokromin kromoforia sitovaa domeenia (CBDmon). Hopeapartikkelien l\u00e4sn\u00e4ollessa CBDmonin kvanttisaanto kasvoi 20 % ja viritetyn tilan elinaika laski 30 %. Syntetisoidun kompleksin kirkkaus verrattuna CBDmonin kirkkauteen oli 470+-40% 390 nm:n ja 134+-11% 630 nm:n virityksell\u00e4. N\u00e4it\u00e4 tuloksia voidaan todenn\u00e4k\u00f6isesti viel\u00e4 kasvattaa, koska kompleksin rakennetta on mahdollista muokata monella eri tavalla. Siksi voidaan todeta, ett\u00e4 kehitetty menetelm\u00e4 on lupaava keino l\u00e4hi-infrapunaalueen leimojen suunnitteluun.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Applications using a cross-disciplinary approach are a common phenomenon in recent scientific research. A good example is the field of living tissue imaging where better biological labels are constantly searched with the help of physics and chemistry. In this Thesis, a near-infrared template combining silver nanoparticles and phytochromes was synthesized. The formation of the complex was confirmed by using optical spectroscopy, electrophoresis and electron microscopy. Promising fluorescent properties were obtained using monomeric chromophore binding domain (CBDmon) of a phytochrome from Deinococcus radiodurans. In the presence of silver nanoparticles, CBDmon showed 20% increased quantum yield and 30% decreased exited state lifetime. The brightness of the synthesized complex was 470+-40% at 390 nm excitation wavelength and 134+-11% at 630 nm relative to the brightness of pure CBDmon. It is likely that these promising properties could be improved by further engineering using variety of methods. Thus, the results indicate a new way to design applicable NIR-labels.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Lauri Nuuttila (lakaalnu) on 2017-09-15 09:13:12.279927. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =True]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2017-09-15T09:13:14Z\r\nNo. of bitstreams: 2\r\nURN:NBN:fi:jyu-201709153737.pdf: 7328089 bytes, checksum: d3d6ee3560fe2fd899683682ec2d4371 (MD5)\r\nlicense.html: 4857 bytes, checksum: feda2dfae457b6bb362c7585ef1fdfab (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2017-09-15T09:13:14Z (GMT). No. of bitstreams: 2\r\nURN:NBN:fi:jyu-201709153737.pdf: 7328089 bytes, checksum: d3d6ee3560fe2fd899683682ec2d4371 (MD5)\r\nlicense.html: 4857 bytes, checksum: feda2dfae457b6bb362c7585ef1fdfab (MD5)\r\n Previous issue date: 2017", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (87 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "plasmonit", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "kirkkaus", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "CBDmon", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "absorptio", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "fluoroforit", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "kvanttisaanto", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "s\u00e4teily", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "elinaika", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "plasmon", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "brightness", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "imaging", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "CBDmon", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "absorption", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "fluorophore", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "quantum yield", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "radiation", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "lifetime", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Interactions between silver nanoparticles and fluorescent phytochromes from Deinococcus radiodurans", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201709153737", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Fysiikan laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Physics", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Fysiikka", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Physics", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2017-09-15T09:13:14Z", "language": "", "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4021", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kuvantaminen", "language": 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