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[{"key": "dc.contributor.advisor", "value": "M\u00e4ntyl\u00e4, Elina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Vihinen-Ranta, Maija", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "J\u00e4rvensivu, Jani", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2017-07-06T11:49:41Z", "language": "", "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2017-07-06T11:49:41Z", "language": "", "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2017", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1704780", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/54879", "language": "", "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Koiran parvovirus (engl. canine parvovirus, CPV) on pieni (halkaisijaltaan ~25 nm), vaipaton protoparvovirus. Sill\u00e4 on ikosahedraalinen kapsidi joka ymp\u00e4r\u00f6i noin 5 kb pitk\u00e4\u00e4, yksijuosteista DNA -genomia. CPV kulkeutuu solun sis\u00e4\u00e4n klatriiniv\u00e4litteell\u00e4 endosytoosilla. Vapauduttuaan endosomeista se kuljetetaan tumaan, jossa genomi replikoidaan ja pakataan uusiin kapsideihin. Lopulta valmiit virukset maturoituvat ja poistuvat tumasta infektoidakseen uusia soluja. Parvovirusten on havaittu tarvitsevan is\u00e4nt\u00e4solunsa solusyklin s\u00e4\u00e4tely\u00e4 edist\u00e4\u00e4kseen genominsa replikaatiota tai uusien partikkeleiden kasaamista. Parvovirusinfektion seurauksena solusykli pys\u00e4htyy S/G2-vaiheeseen. On toistaiseksi ep\u00e4selv\u00e4\u00e4 tarvitsevatko parvovirukset solusyklin s\u00e4\u00e4tely\u00e4 my\u00f6s vapautuakseen is\u00e4nt\u00e4solunsa tumasta. T\u00e4m\u00e4n tutkimuksen tarkoituksena oli selvitt\u00e4\u00e4 CPV:n tumasta vapautumiseen johtavia muutoksia solusykliss\u00e4 ja tumadynamiikassa. Parvoviruksen tumasta vapautumisen tiedettiin ajoittuvan S/G2-vaiheen loppuun, joten solusyklin etenemist\u00e4 kohti M-vaihetta tarkasteltiin kyseiselle siirtym\u00e4lle keskeisen s\u00e4\u00e4telyproteiinin, sykliini B1:n avulla. Aikaisemmissa tutkimuksissa CPV:n on havaittu aiheuttavan katkoksia is\u00e4nt\u00e4solujensa tumakuljetukseen my\u00f6h\u00e4isess\u00e4 vaiheessa infektiota. T\u00e4ss\u00e4 tutkimuksessa pyrittiin selvitt\u00e4m\u00e4\u00e4n liittyv\u00e4tk\u00f6 n\u00e4m\u00e4 ilmi\u00f6t viruksen tumasta poistumiseen ja G2/M -siirtym\u00e4\u00e4n. Tutkimus osoitti ensimm\u00e4ist\u00e4 kertaa, ett\u00e4 CPV:n tumasta poistuminen ajoittuu G2/M \u2013vaiheeseen. Tutkimuksessa havaittiin, ett\u00e4 my\u00f6h\u00e4isen vaiheen CPV-infektio aiheuttaa erityisesti importiini \u03b2:n sek\u00e4 sykliini B1:n, mutta my\u00f6s sykliini B1:n fosforyloitujen muotojen akkumuloitumista tumaan. Virusten marginalisoituessa tumakotelon sis\u00e4pinnalle ennen tumasta poistumistaan, sytoplasmista sykliini B1:t\u00e4 fosforyloitiin seriineist\u00e4 126 ja 147, jonka seurauksena molemmat fosforylaatiot akkumuloituivat samanaikaisesti tumaan. Nopea tuma-akkumulaatio osoittaa solusyklin edenneen G2/M-vaiheeseen. Lis\u00e4ksi S126 ja S147 fosforyoitujen sykliinien m\u00e4\u00e4r\u00e4n todettiin kasvavan infektion edetess\u00e4. N\u00e4m\u00e4 tulokset kertoivat solun v\u00e4hitellen siirtyv\u00e4n G2/M-tarkastuspisteeseen infektion aikana. Tutkimuksessa havaittiin lis\u00e4ksi, ett\u00e4 viruksen poistuessa tumasta sytoplasmaan, S147 fosforyloitu sykliini B1 akkumuloitui tuman sijasta sytoplasmaan, mik\u00e4 kertoo sykliini v\u00e4litteisest\u00e4 G2/M \u2013s\u00e4\u00e4telyst\u00e4 viruksen tumasta poistumisen aikana. Tutkimuksen perusteella on kiistatonta, ett\u00e4 my\u00f6s my\u00f6h\u00e4inen infektio vaikuttaa solusyklin etenemiseen ja G2/M-vaihe on keskeist\u00e4 viruksen tumasta poistumiselle. Lis\u00e4tutkimuksia kuitenkin tarvitaan n\u00e4ihin G2/M -vaihetta edist\u00e4vien signalointireittien selvitt\u00e4miseksi. Erityisesti infektion seurauksena aktivoituvan DNA-vauriovasteen merkitys havaitulle G2/M-siirtym\u00e4lle on t\u00e4rke\u00e4 tulevaisuuden tutkimuskohde.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Canine parvovirus (CPV) is a small (~25 nm in diameter) nonenveloped protoparvovirus. An icosahedral capsid encloses a single stranded DNA genome of ~5 kb in length. CPV internalisation into a host cell occurs via endocytosis followed by transport into the nucleus. Viral genome is replicated and packaged into newly\r\nassembled capsids in the nucleoplasm. Following maturation progeny viruses egress from the nucleus and are\r\nready to infect other cells. Parvoviruses are dependent on the host cell cycle regulation in order to promote\r\ntheir replication or the capsid assembly resulting in S/G2 cell cycle arrest. However, it has remained unknown\r\nwhether they require further cell cycle regulation during their egress from the nucleus.\r\nThe aim of this study was to analyse the role of cell cycle regulation and nuclear dynamics in parvoviral\r\nnuclear egress. The nuclear egress of parvoviruses is known to occur after S/G2. To study if the cell cycle\r\nproceeds towards G2/M during the egress, the intracellular distribution and phosphorylation levels of cyclin\r\nB1, a key regulator of the G2/M transition, was investigated. Recent studies with CPV have indicated that the\r\nlate-infection causes temporal interruptions in the host cell nuclear import. In this study, the relation of these\r\nphenomena on the virus nuclear egress or G2/M-phase transition was evaluated.\r\nThis study shows for the first time that the nuclear egress of CPV occurs in the G2/M \u2013phase of the cell cycle.\r\nThe results indicate that late CPV-infection causes nuclear accumulation of not only importin \u03b2 and cyclin\r\nB1 but also of the serine 126 and 147 phosphorylated forms of cyclin B1. Virus marginalization to the\r\nnucleus periphery near to the inner nuclear membrane prior to nuclear escape was accompanied with cyclin\r\nB1 phosphorylation at S126 and S147 and nuclear accumulation. Moreover, the intranuclear levels of these\r\nphosphorylated cyclins were increased during the progression of the infection showing gradual cell cycle\r\nprogression into G2/M. Virus escape from the nucleus into the cytoplasm was followed by synchronous\r\ncytoplasmic retention of S147P cyclin B1 indicating cyclin-mediated cell cycle regulation during the nuclear\r\negress of parvovirus capsids. Together, these results indicate that the nuclear egress of CPV is accompanied\r\nwith G2/M transition or checkpoint activation. This study shows undisputably that parvoviral infection causes\r\ncell cycle progression from late S/G2 to G2/M. However, further studies are needed to clarify the signalling\r\npathways behind the G2/M phase transition. Especially the effects of parvovirus-induced DNA damage\r\nresponse on the detected G2/M arrest are a significant aspect in future experiments.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Jani J\u00e4rvensivu (janpekja) on 2017-07-06 11:49:40.386323. Form: Pro gradu -lomake (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake). JyX data: [jyx_publishing-allowed (fi) =False]", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija.group@korppi.jyu.fi) on 2017-07-06T11:49:41Z\r\nNo. of bitstreams: 2\r\nURN:NBN:fi:jyu-201707063248.pdf: 5508373 bytes, checksum: ed7444345c9ea8c7ddc9a4a015c54ef1 (MD5)\r\nlicense.html: 1157 bytes, checksum: 9bf643967737af8a295e2a2e5ecfe26e (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2017-07-06T11:49:41Z (GMT). No. of bitstreams: 2\r\nURN:NBN:fi:jyu-201707063248.pdf: 5508373 bytes, checksum: ed7444345c9ea8c7ddc9a4a015c54ef1 (MD5)\r\nlicense.html: 1157 bytes, checksum: 9bf643967737af8a295e2a2e5ecfe26e (MD5)\r\n Previous issue date: 2017", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "1 verkkoaineisto (47 sivua)", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "solusykli", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "sykliini B1", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "tumasta poistuminen", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "canine parvovirus", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "cell cycle regulation", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "solusykli", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "sykliini B1", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "tumasta poistuminen", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "canine parvovirus", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "cell cycle regulation", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "tuma", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Solusyklin G2/M-vaiheen s\u00e4\u00e4telyn vaikutus koiran parvoviruksen tumasta vapautumiseen", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201707063248", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s 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"value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2017-07-06T11:49:41Z", "language": "", "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "parvovirukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "koira", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infektiot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sykliinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "tuma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solubiologia", "language": null, 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