Denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent

Denitrifikaatio ja DNRA (dissimilatory nitrate reduction to ammonium) ovat nitraattia pelkistäviä prosesseja, jotka yleensä esiintyvät hapettomissa olosuhteissa, kuten järvien sedimenteissä ja alusvesissä. Denitrifikaatiossa syntyy typpikaasua, joka poistuu ilmakehään, kun taas DNRA:n lopputuote on...

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Main Author: Hasari, Miikka
Other Authors: Matemaattis-luonnontieteellinen tiedekunta, Faculty of Sciences, Bio- ja ympäristötieteiden laitos, Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylän yliopisto
Format: Master's thesis
Language:eng
Published: 2015
Subjects:
Online Access: https://jyx.jyu.fi/handle/123456789/45373
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author Hasari, Miikka
author2 Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science University of Jyväskylä Jyväskylän yliopisto
author_facet Hasari, Miikka Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science University of Jyväskylä Jyväskylän yliopisto Hasari, Miikka Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science University of Jyväskylä Jyväskylän yliopisto
author_sort Hasari, Miikka
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description Denitrifikaatio ja DNRA (dissimilatory nitrate reduction to ammonium) ovat nitraattia pelkistäviä prosesseja, jotka yleensä esiintyvät hapettomissa olosuhteissa, kuten järvien sedimenteissä ja alusvesissä. Denitrifikaatiossa syntyy typpikaasua, joka poistuu ilmakehään, kun taas DNRA:n lopputuote on ammonium, joka jää veteen. Luonnollinen denitrifikaatio voi poistaa typpeä jätevedenpuhdistamon poistovedestä, mutta DNRA:n rooli on vähemmän tunnettu. Näiden prosessien tutkiminen voi auttaa jätevedenpuhdistuksen kehittämisessä. Tässä Pro Gradu –työssä tutkittiin denitrifikaation ja DNRA:n prosessinopeuksia sekä nopeuksien korrelaatioita erilaisten vedenlaatumuuttujien kanssa. Tutkimuspaikkana oli Keurusselkä –järvi, jonne paikallinen jätevedenpuhdistamo laskee poistovetensä. Metodina käytettiin isotooppileimausta inkubaatio- ja reaktorikokeissa. Järvestä valittiin kolme näytteenottopistettä, joista kaksi sijaitsivat poistovesiputkesta myötävirtaan ja yhtä käytettiin kontrollina. Hypoteeseinä olivat: a) denitrifikaationopeudet ovat suurempia purkuputken läheisyydessä korkeamman nitraattipitoisuuden vuoksi, sekä b) DNRA:denitrifikaatio –suhde on niin ikään suurempi samoissa olosuhteissa. Denitrifikaationopeudet olivat korkeimmillaan purkuputken kohdalla ja ylipäätään suurempia kuin DNRA:n nopeudet. DNRA:denitrifikaatio –suhteessa ei havaittu merkittäviä eroja näytepisteiden tai ajankohtien välillä. Denitrifikaatio korreloi positiivisesti nitraattipitoisuuden kanssa ja negatiivisesti happipitoisuuden sekä pH:n kanssa. DNRA korreloi negatiivisesti pH:n kanssa. Reaktorikokeissa havaittiin aluksi positiivinen korrelaatio denitrifikaation ja veden nitraattipitoisuuden välillä, mutta 24 tunnin inkubaation jälkeen vastaavaa korrelaatiota ei enää esiintynyt. Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are anoxic microbial nitrate-reducing processes, which occur for example in lake sediment and hypolimnion. Denitrification produces N2 gas, which exits the water body, while DNRA produces ammonium, which is retained in the water. Wastewater effluent in a lake can be further purified by natural denitrification, but the role of DNRA is less known. Understanding these processes can help to improve wastewater treatment. In this thesis, rates of denitrification and DNRA and their correlations with water quality were studied in Lake Keurusselkä, where a wastewater treatment plant releases its effluent waters. Incubation and reactor experiments were conducted by using 15N tracers. The hypotheses were that the rates of denitrification are higher when wastewater effluent is present, and that the DNRA:denitrification ratio is also higher in the same conditions. The denitrification rates were highest near the effluent pipe, and in overall higher than those of DNRA. The ratio showed no such trend. Denitrification correlated positively with nitrate concentration and negatively with oxygen concentration and pH, while DNRA showed negative correlation with pH. The reactor experiment results showed initial positive correlation between ambient NO3- levels and denitrification, but not anymore after 24 hours of incubation.
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spellingShingle Hasari, Miikka Denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent DNRA Akvaattiset tieteet Aquatic sciences 4012 järvet denitrifikaatio isotoopit
title Denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent
title_full Denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent
title_fullStr Denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent Denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent
title_full_unstemmed Denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent Denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent
title_short Denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent
title_sort denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent
title_txtP Denitrification and dissimilatory nitrate reduction to ammonium in a lake receiving wastewater effluent
topic DNRA Akvaattiset tieteet Aquatic sciences 4012 järvet denitrifikaatio isotoopit
topic_facet 4012 Akvaattiset tieteet Aquatic sciences DNRA denitrifikaatio isotoopit järvet
url https://jyx.jyu.fi/handle/123456789/45373 http://www.urn.fi/URN:NBN:fi:jyu-201502191346
work_keys_str_mv AT hasarimiikka denitrificationanddissimilatorynitratereductiontoammoniuminalakereceivingwastewater