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[{"key": "dc.contributor.author", "value": "Sillanp\u00e4\u00e4, Kirsikka", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2012-01-23T11:49:24Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2012-01-23T11:49:24Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2011", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1192579", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/37234", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Ihmisten toimesta ymp\u00e4rist\u00f6\u00f6n joutuvien vierasaineiden vaikutukset hormonitoimintaan ovat viime vuosina her\u00e4tt\u00e4neet paljon keskustelua. Yksi alan tutkituimmista aihealueista on estrogeenin kaltaisesti vaikuttavien aineiden eli ymp\u00e4rist\u00f6estrogeenien aiheuttamat lis\u00e4\u00e4ntymis- ja kehitysh\u00e4iri\u00f6t. Estrogeenisi\u00e4 vaikutuksia voidaan tutkia monella tapaa, alkaen solutason vasteista populaatiokehitykseen. Yksi paljon k\u00e4ytetty molekyylimarkkeri on kalojen vitellogeniinitason mittaus. Vaikka normaalioloissa vain naaraat tuottavat vitellogeniini\u00e4, estrogeenialtistuksen vaikutuksesta my\u00f6s koiraat ja nuoret yksil\u00f6t voivat sit\u00e4 tuottaa. T\u00e4ss\u00e4 opinn\u00e4ytety\u00f6ss\u00e4 tutkittiin kolmen ymp\u00e4rist\u00f6estrogeenin, bisfenoli A:n, nonyylifenolin ja oktyylifenolin vaikutusta 20 dpf ik\u00e4isten eli hedelm\u00f6ityksest\u00e4 lukien 20 vuorokauden ik\u00e4isten seeprakalojen (20dpfZF) vitellogeniinin mRNA:n tuottoon. Viisip\u00e4iv\u00e4iset altistuskokeet veteen lis\u00e4tyill\u00e4 aineilla tehtiin sek\u00e4 yksitt\u00e4in ett\u00e4 kolmen aineen NOEC-arvojen seoksena. Tavoitteena oli my\u00f6s selvitt\u00e4\u00e4 20dpfZF-mallin tehokkuutta k\u00e4ytett\u00e4ess\u00e4 vitellogeniinivastetta. \n\nTulosten perusteella 20dpfZF-malli sopii ymp\u00e4rist\u00f6estrogeenien testaamiseen vitellogeniinivasteen avulla, sill\u00e4 positiivisena kontrollina k\u00e4ytetty etinyyliestradioli aiheutti selke\u00e4n annos-vasteriippuvuuden. Bisfenoli A lis\u00e4si vitellogeniinin mRNA:n tuottoa korkeimmalla altistetulla pitoisuudella 2000 \u03bcg/l, mutta nonyylifenolilla tai oktyylifenolilla ei ollut yksitt\u00e4in vaikutusta. Yksitt\u00e4isten annos-vastekokeiden sek\u00e4 kirjallisuusarvojen perusteella yhteisvaikutuskokeessa k\u00e4ytettiin bisfenoli A:n 500 \u03bcg/l, nonyylifenolin 50 \u03bcg/l ja oktyylifenolin 20 \u03bcg/l pitoisuuksia. Kolmen aineen NOEC-arvojen seoksen vaikutus oli pienempi kuin bisfenoli A:n vaikutus yksitt\u00e4in.\n\nYmp\u00e4rist\u00f6estrogeenien aiemmissa tutkimuksissa on yhteisvaikutuksen todettu suurin piirtein noudattavan konsentraatioihin perustuvaa summavaikutusmallia, mutta poikkeuksiakin siit\u00e4 on, kuten osoitti my\u00f6s t\u00e4m\u00e4n ty\u00f6n tulos. Silti kemikaalivalvonnan lains\u00e4\u00e4d\u00e4nn\u00f6ss\u00e4 p\u00e4\u00e4s\u00e4\u00e4nt\u00f6isesti huomioidaan aineiden vaikutukset yksitt\u00e4in, vaikka ymp\u00e4rist\u00f6ss\u00e4 aineet esiintyv\u00e4t monen aineen seoksina. Riskinarvioinnissa tulisi huomioida my\u00f6s t\u00e4m\u00e4.", "language": null, "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Kirsikka Sillanp\u00e4\u00e4 (jekisill) on 2012-01-23 11:49:23.568095. Form: Pro gradu -lomake (1 tekij\u00e4) (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake-1-tekijae). JyX data:", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2012-01-23T11:49:24Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201201231055.pdf: 713648 bytes, checksum: 093ec9c2ebeaec470c1e4c4f459bd9bc (MD5)\nlicense.html: 5047 bytes, checksum: 45c8c984fb9a71a80b27622426f54e83 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2012-01-23T11:49:24Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201201231055.pdf: 713648 bytes, checksum: 093ec9c2ebeaec470c1e4c4f459bd9bc (MD5)\nlicense.html: 5047 bytes, checksum: 45c8c984fb9a71a80b27622426f54e83 (MD5)\n Previous issue date: 2011", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "60 sivua", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "seeprakala", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "vitellogeniini", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mRNA", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "kvantitatiivinen PCR", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "nuoret yksil\u00f6t", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "bisfenoli A", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "nonyylifenoli", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "oktyylifenoli", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "etinyyliestradioli", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Ymp\u00e4rist\u00f6estrogeeniseoksen yhteisvaikutus 20dpfZF-seeprakalan vitellogeniinigeenin ilmentymiseen", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201201231055", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.dcmitype", "value": "Text", "language": "en", "element": "type", "qualifier": "dcmitype", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ymp\u00e4rist\u00f6tiede ja -teknologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Environmental science and technology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.date.updated", "value": "2012-01-23T11:49:24Z", "language": null, "element": "date", "qualifier": "updated", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4015", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "seeprakala", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fenolit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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