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[{"key": "dc.contributor.author", "value": "Lehkonen, Moona", "language": null, "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2011-02-01T13:48:09Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2011-02-01T13:48:09Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2011", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.other", "value": "oai:jykdok.linneanet.fi:1147703", "language": null, "element": "identifier", "qualifier": "other", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/26497", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Solu ottaa sis\u00e4\u00e4ns\u00e4 materiaalia endosytoosin avulla. Koska soluun kuljetettavien molekyylien kirjo on laaja, soluun on kehittynyt erilaisia endosytoosireittej\u00e4. Fagosytoosin avulla soluun kuljetetaan suuria kiinteit\u00e4 partikkeleita. Pinosytoosireittej\u00e4 on useita, ja niiden avulla solu ottaa sis\u00e4\u00e4ns\u00e4 solukalvoa ja solun ulkopuolista nestett\u00e4 sek\u00e4 siihen liuenneita molekyylej\u00e4. \n\nKasvutekij\u00e4reseptorit ovat solukalvon l\u00e4vist\u00e4vi\u00e4 reseptorityrosiinikinaaseja. Kasvutekij\u00e4n sitoutuminen reseptoriinsa saa aikaan reseptorien kasaantumisen, signalointikompleksien muodostumisen sek\u00e4 reseptorin endosytoitumisen. Muodostuneet signalointikompleksit aloittavat soluissa viestiketjuja, jotka lopulta johtavat muutoksiin solujen jakaantumisessa, kasvussa, erilaistumisessa ja liikkumisessa. \n\nIntegriinit ovat solukalvon t\u00e4rkeimpi\u00e4 adheesioreseptoreita, ja ne kiinnitt\u00e4v\u00e4t solut viereisiin soluihin ja soluv\u00e4liaineen proteiineihin. Integriinien kautta tapahtuva signalointi vaikuttaa muun muassa solujen muotoon, kiinnittymiseen ja erilaistumiseen. Integriinit voivat lis\u00e4ksi toimia patogeenireseptoreina. Integriinien ja kasvutekij\u00e4reseptoreiden v\u00e4lill\u00e4 on havaittu yhteisty\u00f6t\u00e4.\n\nEchovirus 1 on ihmisen patogeeni, jonka reseptorina solukalvolla toimii \u03b12\u03b21-integriini. Viruksen sitoutuminen kasauttaa \u03b12\u03b21-integriinit solukalvolla, mink\u00e4 j\u00e4lkeen virus-reseptori -kompleksi endosytoidaan soluun. \n\nT\u00e4m\u00e4n tutkimuksen tarkoituksena oli selvitt\u00e4\u00e4, vaikuttavatko seerumin kasvutekij\u00e4t \u03b12\u03b21-integriinin endosytoosiin ja echovirus 1:n infektiotehokkuuteen. Kasautuneen \u03b12\u03b21-integriinin endosytoositehokkuus selvitettiin kvantitatiivisen konfokaalimikroskopian avulla. Echovirus 1:n infektiivisyytt\u00e4 eri seerumi- ja kasvutekij\u00e4stimulaatioissa tutkittiin fluoresenssimikroskoopilla. Biokemiallisin menetelmin kartoitettiin \u03b12-integriinin, echovirus 1:n ja kasvutekij\u00e4reseptoreiden m\u00e4\u00e4ri\u00e4 soluissa eri seerumi- ja kasvutekij\u00e4stimulaatioissa.\n\nTulosten mukaan kasvutekij\u00e4t edistiv\u00e4t sek\u00e4 \u03b12\u03b21-integriinin endosytoosia ett\u00e4 echovirus 1 -infektiota ihmisen luusy\u00f6p\u00e4soluissa, jotka ilment\u00e4v\u00e4t runsaasti \u03b12\u03b21-integriini\u00e4. N\u00e4iden solujen seerumin\u00e4lkiinnytt\u00e4minen lis\u00e4si joidenkin kasvutekij\u00e4reseptoreiden ilmentymist\u00e4, muttei vaikuttanut \u03b12\u03b21-integriinin m\u00e4\u00e4r\u00e4\u00e4n. My\u00f6s toisessa solulinjassa, ihmisen aortan sileiss\u00e4 lihassoluissa, seerumin\u00e4lkiinnytt\u00e4minen aiheutti joidenkin kasvutekij\u00e4reseptoreiden m\u00e4\u00e4r\u00e4n lis\u00e4\u00e4ntymist\u00e4. Toisin kuin luusy\u00f6p\u00e4soluissa, sileiss\u00e4 lihassoluissa seerumin\u00e4lkiinnytt\u00e4minen kuitenkin tehosti echovirus 1 -infektiota.", "language": null, "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted using Plone Publishing form by Moona Lehkonen (mokalehk) on 2011-02-01 13:48:06.615033. Form: Pro gradu -lomake (1 tekij\u00e4) (https://kirjasto.jyu.fi/julkaisut/julkaisulomakkeet/pro-gradu-lomake-1-tekijae). JyX data:", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by jyx lomake-julkaisija (jyx-julkaisija@noreply.fi) on 2011-02-01T13:48:09Z\nNo. of bitstreams: 2\nURN:NBN:fi:jyu-201102011730.pdf: 2083341 bytes, checksum: 1ae5dc3c6aec6813a1bdf5f1be828a8d (MD5)\nlicense.html: 5012 bytes, checksum: f44d1a753e9eb9be6249fc1b63f64a85 (MD5)", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2011-02-01T13:48:09Z (GMT). No. of bitstreams: 2\nURN:NBN:fi:jyu-201102011730.pdf: 2083341 bytes, checksum: 1ae5dc3c6aec6813a1bdf5f1be828a8d (MD5)\nlicense.html: 5012 bytes, checksum: f44d1a753e9eb9be6249fc1b63f64a85 (MD5)\n Previous issue date: 2011", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "47 sivua", "language": null, "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "kasvutekij\u00e4reseptori", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "\u03b12\u03b21-integriini", "language": null, "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Kasvutekij\u00f6iden merkitys \u03b12\u03b21-integriinin ja echovirus 1:n endosytoosissa", "language": null, "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-201102011730", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.dcmitype", "value": "Text", "language": "en", "element": "type", "qualifier": "dcmitype", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": "fi", "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "endosytoosi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "echovirukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "integriinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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