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[{"key": "dc.contributor.advisor", "value": "Hulmi, Juha", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "M\u00e4ntyselk\u00e4, Sakari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ihalainen, Janne", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Vartiainen, Jennika", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-12-04T06:46:57Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-12-04T06:46:57Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/98790", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Lihakset ovat ihmisille elint\u00e4rkeit\u00e4, sill\u00e4 ne mahdollistavat kehon v\u00e4ltt\u00e4m\u00e4tt\u00f6m\u00e4t toiminnot, kuten hengitt\u00e4misen ja liikkumisen. Lihaskoon s\u00e4\u00e4tely on kompleksinen prosessi, jonka ymm\u00e4rt\u00e4minen voi mahdollistaa lihaskadon hoitomenetelmien kehitt\u00e4misen. T\u00e4ss\u00e4 Pro gradu -tutkielmassa tutkittiin eri tapoja lis\u00e4t\u00e4 viljeltyjen ihmislihassolujen proteiinisynteesi\u00e4 sek\u00e4 n\u00e4iden lihassolujen glukoosin k\u00e4ytt\u00f6\u00e4 biomolekyylien synteesiss\u00e4. Viljellyille ihmislihassoluille tehtiin solukokeita tarkoituksena lis\u00e4t\u00e4 lihassolujen proteiinisynteesi\u00e4. Lihassoluista eristettiin proteiinit ja niiden proteiinikonsentraatiot mitattiin. Nestetuikelaskennan avulla tutkittiin lihassolujen sis\u00e4lt\u00e4mien proteiinien m\u00e4\u00e4ri\u00e4 sek\u00e4 glukoosin k\u00e4ytt\u00f6\u00e4 proteiinien, RNA:n ja lipidien synteesiss\u00e4. Seriinisynteesireitill\u00e4 toimivan entsyymin, 3-fosfoglyseraattidehydrogenaasin (eng. Phosphoglycerate dehydrogenase, PHGDH), sek\u00e4 Rapamysiinin mekanistisen kohteen 1 (eng. The mechanistic target of rapamycin complex 1, mTORC1) aktiivisuudesta kertovien fosforyloituneiden S6K1- ja S6-proteiinien m\u00e4\u00e4ri\u00e4 n\u00e4ytteiss\u00e4 tutkittiin Western blotin avulla. Tuloksia analysoitiin Western blotin membraanikuvia hy\u00f6dynt\u00e4en. Seerumistimulaatio ja insuliinin kaltainen kasvutekij\u00e4 1 (eng. Insulin-like growth factor 1, IGF-1) lis\u00e4siv\u00e4t viljeltyjen ihmislihassolujen proteiinisynteesi\u00e4. Seerumistimulaatiossa lihassolujen medium sis\u00e4lsi enemm\u00e4n kasvutekij\u00f6it\u00e4 suhteessa kontrolliryhm\u00e4\u00e4n. Seerumistimulaatio lis\u00e4si 14C-glukoosin hiiliatomien kertymist\u00e4 proteiineihin ja RNA:han, kun taas insuliinin kaltainen kasvutekij\u00e4 1 lis\u00e4si 14C-glukoosin hiiliatomien kertymist\u00e4 RNA:han ja lipideihin. Tulokset viittasivat siihen, ett\u00e4 seerumistimulaatiolla ja insuliinin kaltaisella kasvutekij\u00e4 1:ll\u00e4 voi olla kyky\u00e4 lis\u00e4t\u00e4 viljeltyjen ihmislihassolujen kasvua, mik\u00e4 on t\u00e4rke\u00e4 tulos lihaskadon hoitomenetelmiin liittyvien jatkotutkimusten kannalta. Tulevaisuuden tutkimuksissa tulisi selvitt\u00e4\u00e4, millainen vaikutus glukoosilla on biomassan synteesiss\u00e4 lihaksessa ja voidaanko n\u00e4it\u00e4 biosynteesireittej\u00e4 hy\u00f6dynt\u00e4\u00e4 lihaskadon hoitomenetelmien kehitt\u00e4misess\u00e4.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Muscles are essential for humans, because they allow the necessary functions of the body like breathing and movements. The stimulation of muscle size is a complex process, but understanding it is crucial for the development of treatment methods for patients suffering from muscle loss. In this Master\u2019s thesis, we investigated how to increase the protein synthesis of cultured human muscle cells and how glucose is utilized in the biomolecule synthesis of these muscle cells. We cultured human muscle cells and accomplished cell tests to increase the protein synthesis. We isolated proteins from the muscle cells and measured protein concentrations. Liquid scintillation counting was used to measure protein contents and study the incorporation of glucose into proteins, RNAs and lipids. We utilized Western blotting to discover possible differences in rates of phosphoglycerate dehydrogenase enzyme (PHGDH) and phosphorylated proteins S6K1 and S6. Phosphoglycerate dehydrogenase enzyme is an essential enzyme in serine synthesis pathway, and phosphorylated proteins S6K1 and S6 indicate the activation of the mechanistic target of rapamycin complex 1 (mTORC1). The results were analyzed using Western blotting membrane photos. Serum stimulation and insulin-like growth factor 1 (IGF-1) increased the protein synthesis of the muscle cells. In serum stimulation the medium of the muscle cells contained more growth factors compared to the medium of the control cells. Serum stimulation caused an increase in incorporation of 14C glucose carbon atoms into proteins and RNAs. Insulin-like growth factor 1 increased the incorporation of those carbon atoms into RNAs and lipids. Our results suggested that serum stimulation and insulin-like growth factor 1 may have potential to increase the growth of the cultured human muscle cells, which is an important result for further studies of treatment methods for muscle loss. In the future, it is important to study how significant role glucose has in the biomolecule synthesis of the muscle and whether these biosynthesis pathways can be utilized in the development of treatment methods for muscle loss.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2024-12-04T06:46:57Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-12-04T06:46:57Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "42", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "hypertrofia", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "insuliinin kaltainen kasvutekij\u00e4 1", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mTORC1", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "seerumistimulaatio", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "proteiinisynteesi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Viljeltyjen ihmislihassolujen proteiinisynteesin stimulointi ja glukoosin k\u00e4ytt\u00f6 biomolekyylien synteesiss\u00e4", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202412047609", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "lihakset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "glukoosi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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