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[{"key": "dc.contributor.advisor", "value": "Pekkala, Satu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ruuskanen, Suvi", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Peltonen, Olivia", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-10-24T05:21:10Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-10-24T05:21:10Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/97657", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Paksusuolen sy\u00f6p\u00e4 voidaan jakaa p\u00e4\u00e4kasvaimen anatomisen sijainnin perusteella oikean- ja vasemmanpuoleiseen paksusuolen sy\u00f6p\u00e4\u00e4n, jotka eroavat toisistaan esimerkiksi tyypillisten riskitekij\u00f6iden, oirekuvan sek\u00e4 kuolleisuuden perusteella. Paksusuolen sy\u00f6p\u00e4\u00e4n liittyy usein erilaisia muutoksia limakalvojen musiinikerroksessa sek\u00e4 suolistomikrobiston rakenteessa. N\u00e4iden muutosten uskotaan olevan yhteydess\u00e4 my\u00f6s paksusuolen sy\u00f6v\u00e4n puoleisuuseroihin, mutta aiemmissa tutkimuksissa saadut tulokset aiheesta ovat ristiriitaisia. Koska paksusuolen sy\u00f6v\u00e4n puoleisuuden ja siihen liittyvien kliinisten ominaisuuksien taustalla vaikuttavat monimutkaiset syy-seuraussuhteet ovat viel\u00e4 ep\u00e4selvi\u00e4, olisi t\u00e4rke\u00e4\u00e4 ymm\u00e4rt\u00e4\u00e4 paremmin sy\u00f6v\u00e4n syntyyn ja kehitykseen vaikuttavia mikrobiaalisia ja molekulaarisia tekij\u00f6it\u00e4. T\u00e4m\u00e4n tutkielman tarkoituksena on tuottaa lis\u00e4\u00e4 tietoa aiheesta vertaamalla musiinien ekspressiota sek\u00e4 suolistomikrobiston koostumusta vasemman- ja oikeanpuoleisessa paksusuolen sy\u00f6v\u00e4ss\u00e4 sek\u00e4 terveess\u00e4 paksusuolessa. Tutkimuksessa k\u00e4ytettiin sy\u00f6p\u00e4potilaiden kasvaimista ja terveest\u00e4 paksusuolesta ker\u00e4ttyj\u00e4 kudosn\u00e4ytteit\u00e4, sek\u00e4 n\u00e4ilt\u00e4 koehenkil\u00f6ilt\u00e4 ja terveilt\u00e4 kontrollihenkil\u00f6ilt\u00e4 ker\u00e4ttyj\u00e4 ulosten\u00e4ytteit\u00e4. Kudosten ja musiinikerroksen rakennetta tutkittiin immunohistologisen v\u00e4rj\u00e4yksen avulla. Musiinien ekspressiota kudoksissa tutkittiin dPCR:n avulla kvantifioimalla nelj\u00e4n t\u00e4rkeimm\u00e4n MUC-mRNA:n ekspressiota kudosn\u00e4ytteiss\u00e4. Lis\u00e4ksi kasvaimista ja ulosten\u00e4ytteist\u00e4 eristetty\u00e4 bakteriaalista DNA:ta k\u00e4ytettiin suolistomikrobiston koostumuksen selvitt\u00e4miseen ryhmiss\u00e4 16S rRNA-geenisekvensoinnin avulla. T\u00e4ss\u00e4 tutkielmassa ei l\u00f6ydetty yhteytt\u00e4 MUC-ekspression ja paksusuolen sy\u00f6v\u00e4n tai sen puoleisuuden v\u00e4lill\u00e4. My\u00f6sk\u00e4\u00e4n merkitsevi\u00e4 eroja alfadiversiteetiss\u00e4 ei l\u00f6ytynyt ryhmien v\u00e4lill\u00e4. Sen sijaan beetadiversiteetti oli hyvin erilainen sy\u00f6p\u00e4potilaiden ja verrokkien v\u00e4lill\u00e4. N\u00e4iden ryhmien, mutta my\u00f6s oikean- ja vasemmanpuoleisten paksusuolen sy\u00f6pien suolistomikrobiston v\u00e4lilt\u00e4 l\u00f6ytyi monia taksonomisia eroavaisuuksia. Tutkielmassa saadut tulokset ovat alustavia, mutta ne osoittavat, ett\u00e4 aiheeseen liittyv\u00e4\u00e4 jatkotutkimusta suuremmalla otoksella ja monipuolisempia n\u00e4ytteit\u00e4 verraten tarvitaan. Tietoa paksusuolen sy\u00f6v\u00e4n puoleisuuseroista voitaisiin tulevaisuudessa k\u00e4ytt\u00e4\u00e4 esimerkiksi apuna yksil\u00f6llisempien sy\u00f6p\u00e4hoitojen ja luotettavampien seulontamenetelmien kehitt\u00e4misess\u00e4 sek\u00e4 paksusuolen sy\u00f6v\u00e4n ennaltaehk\u00e4isyss\u00e4.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Colon cancer can be divided into right- and left-sided colon cancer based on the anatomical location of the primary tumor. Colon cancer sidedness is linked to differential clinical characteristics such as risk factors, incidence, symptoms, therapy response, and survival. Colon cancer is associated with changes in the mucosal layer, including mucin proteins, and the gut microbiota. The relationship between colon cancer sidedness and the changes in mucins as well as the gut microbiota composition remains unclear. Because of the previously established association between primary tumor location and many clinical characteristics of colon cancer, understanding the microbial and molecular background of colon cancer development is very important. This thesis aims to obtain more information on the subject by comparing mucin expression and the gut microbiota composition in left- and right-sided colon cancers and healthy colon. Tissue samples from tumors and normal adjacent colon from subjects with right- and left-sided colon cancer, and faecal samples from these subjects as well as healthy control subjects were used in this thesis. Immunohistochemistry was used to observe the differences in the tissues and their mucosal layer in groups. The expression of four most relevant MUC mRNAs in the tissue samples was quantified using dPCR to compare the mucin expression between the groups. Tumors and faecal samples were then used to isolate and amplify the bacterial DNA in 16S rRNA gene sequencing to compare the gut microbiota composition between the groups. In this thesis, no statistically significant differences in MUC expression were found between the colon cancer and normal adjacent tissue, or between right- and left-sided colon cancer. Alpha diversity also didn\u2019t differ between groups. However, beta diversity was significantly different in the faecal samples from colon cancer patients and the healthy controls. Multiple taxonomical differences were also found between these groups, as well as the right- and left-sided colon cancer groups. Ultimately, the preliminary results obtained in this thesis suggest that further research using a larger sample size, and comprehensive samples is needed. In the future, information on the relationship between mucins, the gut microbiota and colon cancer sidedness could potentially be used in the development of more individualized treatments, and more reliable screening for colon cancer, and to efficiently prevent the formation of colon cancer.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2024-10-24T05:21:10Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-10-24T05:21:10Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "58", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "MUC1", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MUC2", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MUC5AC", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MUC6", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mucous membranes", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The link between intestinal mucins, the gut microbiota, and colon cancer sidedness", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202410246514", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sy\u00f6p\u00e4taudit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "paksusuolisy\u00f6p\u00e4", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suoliston taudit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suolisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suolistosy\u00f6v\u00e4t", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "paksusuoli", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suolistomikrobisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cancerous diseases", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cancer of the large intestine", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "intestinal diseases", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "intestines", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bowel cancer", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microbiome", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "large intestine", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microbes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "gastrointestinal microbiota", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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