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[{"key": "dc.contributor.advisor", "value": "Kalenius, Elina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "M\u00e4ntyselk\u00e4, Sakari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Liimatainen, Roosa-Maria", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-08-15T10:58:21Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-08-15T10:58:21Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/96618", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "T\u00e4m\u00e4n Pro gradu -tutkimuksen kirjallisessa osassa perehdyt\u00e4\u00e4n pintaa syvemm\u00e4lle solujen \nmetabolomiikkaan sek\u00e4 fluksomiikkaan. Kirjallisen osan alussa k\u00e4sitell\u00e4\u00e4n solujen toimintaa \nperustasolla ja k\u00e4yd\u00e4\u00e4n l\u00e4pi tutkimuksen osalta t\u00e4rkeimpi\u00e4 aineenvaihduntareittej\u00e4 ja niiden \ntuottamia aineenvaihduntatuotteita eli metaboliitteja. T\u00e4m\u00e4n j\u00e4lkeen kirjallinen osa keskittyy \nk\u00e4sittelem\u00e4\u00e4n fluksomiikkaa syvemm\u00e4ll\u00e4 tasolla ja sit\u00e4, kuinka isotooppileimaus on isossa \nroolissa sen tutkimuksessa. N\u00e4iden edell\u00e4 mainittujen aiheiden lis\u00e4ksi kirjallisessa osassa \nk\u00e4sitell\u00e4\u00e4n nykyp\u00e4iv\u00e4n\u00e4 metabolomiikan ja fluksomiikan tutkimuksessa yleisimmin k\u00e4ytettyj\u00e4 \nanalyysimenetelmi\u00e4, kuten massaspektrometriaa, nestekromatografiaa, ydinmagneettista \nresonanssispektroskopiaa sek\u00e4 kaasukromatografiaa. Kirjallisen osan loppupuolella k\u00e4sitell\u00e4\u00e4n \ntutkimuksessa keskeisess\u00e4 roolissa olevia analyysimenetelmien yhdistelmi\u00e4, kuten LC-MS- ja \nGC-MS-analytiikkaa. \n\nKokeellisen osan tarkoituksena oli perehty\u00e4 ihmisen sek\u00e4 hiiren lihassolun\u00e4ytteiden \nsoluviljelyyn, n\u00e4ytteiden ker\u00e4ykseen sek\u00e4 n\u00e4ytteiden analysoitiin UHPLC-QQQ-MS-laitteistoa \nhy\u00f6dynt\u00e4en. Tutkimuksessa perehdyttiin syvemmin, kuinka lihassolujen aineenvaihduntareitit \ntuottavat tiettyj\u00e4 kiinnostuksen kohteena olevia aminohappoja sek\u00e4 kuinka nopeasti ne tuottavat \nniit\u00e4 tietyiss\u00e4 aikapisteiss\u00e4. N\u00e4ytteiden analysoinnin osalta tutkimuksessa keskityttiin ensin \noptimoimaan UHPLC-MS/MS-menetelm\u00e4\u00e4 kuoppatestin sek\u00e4 aikapistevertailun avulla. Kun \nmenetelm\u00e4 oli saatu optimoitua mittauksiin sopivaksi, analysoitiin ihmisen lihassolun\u00e4ytteist\u00e4 \naineenvaihduntareittien virtausnopeutta eli fluksomiikkaa. Fluksomiikan tutkimisessa \nhy\u00f6dynnetiin isotooppileimausta, jossa ihmisen lihassolujen annettiin vaikuttaa 13C isotooppileimatun glukoosiliuoksen kanssa eri aikapisteiden verran. Isotooppileimattuja \nglukoosiliuoksia valmistettiin kokeellista osaa varten kahta erilaista, joissa niiden pitoisuudet \ns\u00e4\u00e4dettiin HS (horse serum) - ja FBS (fetal bovine serum) -seerumien avulla 2 ja 20 \nprosenttisiksi. Eri seerumipitoisten gluukosiliuosten tarkoituksena oli stimuloida ihmisen \nlihassolujen aineenvaihduntareittej\u00e4 ja tarkoituksena oli analysoida, onko isotooppileimatun \nglukoosiliuoksen v\u00e4kevyydell\u00e4 merkitt\u00e4v\u00e4\u00e4 vaikutusta aminohappojen tuotantoon.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2024-08-15T10:58:21Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-08-15T10:58:21Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "92", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.title", "value": "Aineenvaihduntareittien fluksomiikan analysointi lihassoluista UHPLC-MS-menetelm\u00e4ll\u00e4", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202408155502", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Kemian laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Chemistry", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Analyyttinen kemia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Analytical Chemistry", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "40311", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aineenvaihduntatuotteet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "analyysi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "lihassolut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kemia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suuren erotuskyvyn nestekromatografia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "massaspektrometria", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "soluviljely", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "analyysimenetelm\u00e4t", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "nestekromatografia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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