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[{"key": "dc.contributor.advisor", "value": "Hettunen, Kaisa", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Matsumoto-Viljanen, Yomi", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Rauhala, Pirre", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-08-12T07:02:15Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-08-12T07:02:15Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/96567", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Samepisteuutto on esikonsentrointi ja puhdistusmenetelm\u00e4, jota k\u00e4ytet\u00e4\u00e4n sek\u00e4 ep\u00e4orgaanisten ett\u00e4 orgaanisten analyyttien uuttamiseen. Se on kehitetty alun perin metalli-ioneille mutta sen tutkimuskohteita on laajennettu koskemaan my\u00f6s monenlaisia orgaanisia yhdisteit\u00e4. Tutkimukseni kirjallisessa osassa keskityn samepisteuuttoon orgaanisessa analytiikassa. Tarkoitukseni oli selvitt\u00e4\u00e4 mink\u00e4laisiin yhdisteryhmiin ja erilaisiin matriiseihin menetelm\u00e4\u00e4 on k\u00e4ytetty, miten sit\u00e4 on modifioitu sek\u00e4 miten yhdisteiden kemialliset ominaisuudet vaikuttavat samepisteuuton onnistumiseen.\nKokeellisessa osassa keskityin tutkimaan samepisteuuton parametrien optimointia kversetiinin sek\u00e4 ellagihapon m\u00e4\u00e4rityksiin ja detektointiin UV-VIS spektrofotometrill\u00e4. Optimoin Genapol X080 surfaktantin konsentraation, pH:n, suolan ja sen pitoisuuden sek\u00e4 tasapainoajan sek\u00e4 testasin my\u00f6s nelj\u00e4n eri surfaktantin vaikutusta uuttoon. Optimaalisiksi olosuhteiksi kversetiinin m\u00e4\u00e4ritt\u00e4miseksi saatiin Genapolin pitoisuus 5%, suolana 4% Na2SO4, pH 5 ja tasapainoaika 15 min. Ellagihapolle oli tarkoitus tehd\u00e4 vastaavat optimoinnit, mutta ellagihapon saannot j\u00e4iv\u00e4t eri surfaktanteilla ja eri pH arvoilla vain 20\u201330% joten todettiin ett\u00e4 ellagihapon soveltuvuutta samepisteuuttoon tulisi tutkia enemm\u00e4n. Lopuksi testattiin viel\u00e4 samepisteuuttoa optimoiduissa olosuhteissa pihlajanmarjoista kokonaispolyfenolim\u00e4\u00e4r\u00e4n ja C-vitamiinin m\u00e4\u00e4ritt\u00e4miseksi Folin-Ciocalteu menetelm\u00e4ll\u00e4 ja verrattiin tuloksia saman aikaisesti tehtyyn neste-nesteuutton\u00e4ytteeseen. Kokonaisfenolien tutkimuksessa saatiin neste-nesteuutolla paremmat tulokset kuin CPE:ll\u00e4. T\u00e4m\u00e4 oletetaan johtuvan siit\u00e4, ett\u00e4 osa polyfenoleista on enemm\u00e4n vesiliukoisia kuin toiset, joten osa polyfenoleista on voinut j\u00e4\u00e4d\u00e4 samepisteuutossa vesifaasiin, jolloin surfaktanttifaasin polyfenolim\u00e4\u00e4r\u00e4 j\u00e4\u00e4 virheellisen pieneksi.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2024-08-12T07:02:15Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-08-12T07:02:15Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "100", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.title", "value": "Samepisteuutto orgaanisessa analytiikassa", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202408125443", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Kemian laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Chemistry", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Analyyttinen kemia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Analytical Chemistry", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "40311", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kemia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "uutto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "orgaaniset yhdisteet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "pinta-aktiiviset aineet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "menetelm\u00e4t", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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