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[{"key": "dc.contributor.advisor", "value": "Jokela, Tiina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Johnasson, Andreas", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Littunen, Henna-Riikka", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-06-26T10:29:23Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-06-26T10:29:23Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/96166", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "DNA:n korjausgeenien mutaatiosta aiheutuva Lynchin syndrooma (LS) on perinn\u00f6llinen sy\u00f6pien kehittymiselle altistava sairaus. LS kantajien sy\u00f6p\u00e4riskiin vaikuttavia tekij\u00f6it\u00e4 ei viel\u00e4 ole kunnolla tutkittu. Epigeneettisten tekij\u00f6iden, kuten ei-koodaavien verenkierron mikro-RNA:den (cmiR), jotka s\u00e4\u00e4telev\u00e4t geenien ilmenemist\u00e4, on kuitenkin todettu vaikuttavan sy\u00f6p\u00e4riskiin. Tutkimuksissa on havaittu tiettyjen cmiR:iden ilmenemistasojen poikkeavan LS:n kantajilla verrattuna terveisiin ei-kantajiin. LS kantajien cmiR tasot olivat hyvin samankaltaiset kolorektaalisy\u00f6p\u00e4potilailta tutkittujen cmiR tasojen kanssa, mik\u00e4 viittaa siihen, ett\u00e4 l\u00f6ydetyt miR:t voisivat vaikuttaa sy\u00f6v\u00e4n kehittymiseen tai torjuntaan. Koska valkosolut toimivat sy\u00f6v\u00e4n torjunnassa, on mahdollista, ett\u00e4 n\u00e4m\u00e4 LS:ssa poikkeavissa m\u00e4\u00e4rin ilmenev\u00e4t verenkierron miR:t (LS cmiR:t) vaikuttaisivatkin sy\u00f6p\u00e4riskiin valkosolujen kautta. T\u00e4m\u00e4n ty\u00f6n tarkoituksena oli kehitt\u00e4\u00e4 menetelm\u00e4 cmiR:iden ilmentymism\u00e4\u00e4rien tutkimiseen erityyppisiss\u00e4 valkosoluissa, ja siten tarkastella ilment\u00e4v\u00e4tk\u00f6 valkosolut my\u00f6s LS cmiR:ita. T\u00e4t\u00e4 varten terveilt\u00e4 ihmisilt\u00e4 otetuista verin\u00e4ytteist\u00e4 eristettiin valkosolut ja ne lajiteltiin tyypeitt\u00e4in erillisiksi solupopulaatioiksi virtaussytometrilla. Solujen miR:t eristettiin ja qPCR:n avulla tutkittiin sis\u00e4lsiv\u00e4tk\u00f6 n\u00e4ytteet seuraavia LS cmiR:ita: miR-339-5p, let-7e-5p, miR-451a, miR-320a ja miR-15a-5p. Tulokset osoittivat, ett\u00e4 valkosolut ilmensiv\u00e4t kaikkia edell\u00e4 mainituista cmiR:ista. miR-320a tasot olivat lis\u00e4ksi huomattavia eri valkosolutyypeiss\u00e4, erityisesti neutrofiileiss\u00e4. T\u00e4m\u00e4 voisi viitata siihen, ett\u00e4 miR-320a s\u00e4\u00e4telisi neutrofiilien toimintaa. Lis\u00e4ksi ty\u00f6ss\u00e4 tutkittiin vaikuttaako miR-15a-5p sy\u00f6v\u00e4n kehitykseen yhdistetyn ja tulehdusta aiheuttavan viestiaineen, interleukiini-1\u03b2:n (IL-1\u03b2) s\u00e4\u00e4telyyn. miR-15a-5p:t\u00e4 siirrettiin viljeltyihin ihmisen valkosoluihin, IL-1\u03b2:n tuotto k\u00e4ynnistettiin lis\u00e4\u00e4m\u00e4ll\u00e4 viljelmiin lipopolysakkaridia ja IL-1\u03b2:n geenien ilmentymistasoja valkosoluissa tutkittiin qPCR:lla. Selke\u00e4\u00e4 muutosta IL-1\u03b2:n ilmenemisess\u00e4 miR-15a-5p sis\u00e4lt\u00e4viss\u00e4 soluissa verrattuna kyseist\u00e4 cmiR:ta sis\u00e4lt\u00e4m\u00e4tt\u00f6miin soluihin ei kuitenkaan havaittu. Tutkimus osoitti kehitettyjen menetelmien kuitenkin olevan toimivia samankaltaisiin tutkimustarkoituksiin osoittaen valksolujen ja LS cmiR:iden v\u00e4lisen yhteyden ja sen tarkemman tutkimisen t\u00e4rkeyden tulevaisuudessa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Lynch Syndrome (LS), caused by mutations in DNA mismatch repair genes, is an inherited cancer predisposition increasing the risk of developing cancer already at young age. Factors affecting the cancer risk in LS are still understudied. Epigenetic factors such as circulating micro-RNAs (cmiRs) \u2013 non-coding RNAs that epigenetically regulate gene expression and travel in blood \u2013 have been described to affect that risk. It has been demonstrated that cancer free LS carriers have distinct cmiR profiles from healthy individuals but share similarities with colorectal cancer patients. As white blood cells, also referred as leukocytes, participate in cancer prevention it could be possible that these differentially expressed cmiRs affect cancer risk and development through leukocytes. The aims of this thesis were to develop a method for studying miR expression in different leukocyte types and for examining whether miR-15a-5p would regulate gene expression of inflammation promoting and possibly cancer associated molecule interleukin-1\u03b2 in leukocytes. For the first aim leukocytes were extracted from the human blood of healthy individuals, and different leukocyte types sorted with a flow cytometer. The miRs were then extracted from sorted subpopulations and expression levels of miR-339-5p, let-7e-5p, miR-451a, miR-320a and miR-15a-5p studied with qPCR. Results revealed that leukocytes expressed all of these cmiRs. miR-320a showed significant expression in all leukocyte subtypes. Especially neutrophils showed prominent miR-320a expression suggesting it could have significance in neutrophil functions related regulation. For the second aim, miR-15a-5p was transfected into human leukocyte culture and production of interleukin-1\u03b2 triggered with lipopolysaccharides. Gene expression levels of interleukin-1\u03b2 in miR transfected and non-transfected cultures were examined with qPCR, but clear difference was not detected. Results indicated that more repetitions are required for better evaluation. Nevertheless, the designed protocols proved functional for the purposes and this study strengthened the connection between cmiRs and leukocytes indicating importance for further investigations.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2024-06-26T10:29:23Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-06-26T10:29:23Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "59", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "cell sorting", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "cmiR", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "flow cytometry", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "FACS", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "immune cells", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "leukocytes", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "micro-RNA", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "FACS-based Phenotype Analysis of White Blood Cells : Interplay with Lynch Syndrome Related Circulating Micro-RNAs", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202406265010", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sytokiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "valkosolut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "Lynchin oireyhtym\u00e4", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "RNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geeniekspressio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cytokines", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "white blood cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "Lynch syndrome", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "RNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "gene expression", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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