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[{"key": "dc.contributor.advisor", "value": "Marjom\u00e4ki, Varpu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Laajala, Mira", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Westerholm, Anni", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-06-24T11:40:58Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-06-24T11:40:58Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/96130", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Kasvin solukossa symbioosissa el\u00e4v\u00e4t endofyyttisienet tuottavat suuren m\u00e4\u00e4r\u00e4n bioaktiivisia aineita, joiden avulla ne muun muassa osallistuvat kasvin immuunipuolustukseen ja tuholaisten torjuntaan. Maapallon kaikista sienilajeista tunnetaan vain murto-osa ja niiden tuottamissa metaboliatuotteissa on suuri potentiaali antiviraalisten yhdisteiden l\u00f6yt\u00e4miseksi. Tutkielmassa etsittiin kuudesta eri endofyyttisienin\u00e4ytteest\u00e4 antiviraalista vaikutusta HCov-OC43-koronavirukselle ja CVA9-enterovirukselle. Molemmat virukset ovat yksijuosteisia RNA-viruksia ja niit\u00e4 vastaan on kehitetty vain muutamia rokotteita. K\u00e4ytetyist\u00e4 n\u00e4ytteist\u00e4 ei l\u00f6ydetty antiviraalista vaikutusta koronavirukselle, mutta enterovirukselle yhdell\u00e4 sienin\u00e4ytteell\u00e4 (E12117 MY) n\u00e4htiin antiviraalista vaikutusta erityisesti suuremmilla pitoisuuksilla. T\u00e4m\u00e4n n\u00e4ytteen vaikutusta CVA9-viruksen kapsidirakenteeseen ja kulkeutumista soluissa tutkittiin lis\u00e4\u00e4. Sulamispistett\u00e4 tutkimalla havaittiin virusta stabiloiva vaikutus, joka n\u00e4htiin k\u00e4sittelyn j\u00e4lkeen sulamispisteen nousuna. Stabilisaatiota tuki my\u00f6s koe, miss\u00e4 viruskapsidin reaaliaikaista avautumista tutkittiin. Koe perustui SYBR Green II -v\u00e4rin sitoutumiseen viruksesta vapautuvaan RNA:han. T\u00e4m\u00e4n lis\u00e4ksi radioaktiivisesti leimatun viruksen signaalia seuraamalla oletettiin endofyyttisienin\u00e4ytteen vaikuttavan viruksen rakenteeseen aggregoivasti. T\u00e4t\u00e4 oletusta tuki my\u00f6s l\u00e4pivalaisuelektronimikroskoopilla otetut kuvat viruksesta. E12117 MY -n\u00e4ytteen vaikutusta CVA9-viruksen kulkeutumiseen soluissa tutkittiin konfokaalimikroskoopilla ja havaittiin, ett\u00e4 n\u00e4ytteell\u00e4 ei ollut siihen vaikutusta. Yhdess\u00e4 n\u00e4m\u00e4 tulokset osoittavat, ett\u00e4 endofyyttisienest\u00e4 E12117 MY l\u00f6ytyy potentiaalista antiviraalista vaikutusta CVA9-virusta vastaan ja t\u00e4m\u00e4 vaikutus perustuu viruksen lis\u00e4\u00e4ntyneeseen stabiilisuuteen. Sienin\u00e4ytteen fraktiointi ja vaikuttavan yhdisteen tai yhdisteiden identifiointi voisi olla seuraava tutkimusvaihe sienin\u00e4ytteen antiviraalisuutta tutkittaessa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2024-06-24T11:40:58Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-06-24T11:40:58Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "43", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "antiviraaliset aineet", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "coxsackievirus", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Endofyyttisienin\u00e4ytteiden antiviraalinen vaikutus korona- ja enteroviruksilla", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202406244976", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "endofyytit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "koronavirukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sienet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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