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[{"key": "dc.contributor.advisor", "value": "Watts , Phillip", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Brila, Ilze", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Sipola, Hanna", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-06-24T07:48:48Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-06-24T07:48:48Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/96109", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Ionisoivalla s\u00e4teilylle altistuminen voi aiheuttaa soluvaurioita ja siten vaikuttaa is\u00e4nn\u00e4n terveyteen, kuten muuttamalla suolistomikrobistoa. Mets\u00e4myyr\u00e4 (Clethrionomys glareolus) on uudelleen asuttanut Chornobyl Exclusion Zone (CEZ) -aluetta tapahtuneen ydinvoimalaonnettomuuden j\u00e4lkeen, mik\u00e4 tarjoaa ainutlaatuisen mahdollisuuden tutkia kroonisen pieniannoksisen s\u00e4teilyn pitk\u00e4aikaisia biologisia vaikutuksia. On olemassa tutkimuksia, jotka osoittavat, ett\u00e4 suurille s\u00e4teilyannoksille altistuneiden mets\u00e4myyrien suolistomikrobisto on muuttunut. Ei kuitenkaan tiedet\u00e4, vaikuttaako n\u00e4m\u00e4 suolistomikrobiston muutokset is\u00e4nn\u00e4n terveyteen muuttamalla suolistomikrobistojen tuottamia metaboliitteja. Esimerkiksi, mit\u00e4 l\u00e4heisemp\u00e4\u00e4 sukua mikrobit ovat toisilleen, sit\u00e4 samankaltaisemmat ovat niiden is\u00e4nn\u00e4lle tarjoamat hy\u00f6dyt, kuten metaboliittien tuotanto. Asian tutkimiseksi mets\u00e4myyrien ulosten\u00e4ytteit\u00e4 ker\u00e4ttiin saastuneilta alueilta CEZ:ss\u00e4 sek\u00e4 saastumattomilta alueilta CEZ:n ja Kiovan l\u00e4helt\u00e4. N\u00e4ytteet sek\u00e4 niiden mikrobi ett\u00e4 metaboliitti koostumukset ker\u00e4ttiin osana Earth Microbiome 500 -projektia. Lyhyesti, mikrobiota tunnistettiin k\u00e4ytt\u00e4m\u00e4ll\u00e4 16S rRNA v4 amplikoni sekvensointia, saatu data k\u00e4siteltiin QIIME2:lla ja analysointi suoritettiin PHYLOSEQ ja VEGAN ohjelmapaketeilla. Metaboliitit m\u00e4\u00e4riteltiin kohdistamattomalla metabolomiikalla (ultra-korkean suorituskyvyn nestekromatografia yhdistettyn\u00e4 massaspektrometriin), saatu data k\u00e4siteltiin MZmine ohjelmalla ja analysointi suoritettiin PHYLOSEQ ja VEGAN ohjelmapaketeilla. Havaitsin, ett\u00e4 (1) s\u00e4teily vaikutti suolistomikrobiston alfa ja beeta diversiteetteihin, mutta ei metaboliittien ja (2) n\u00e4ill\u00e4 muutoksilla ei ollut assosiaatiota kesken\u00e4\u00e4n. N\u00e4m\u00e4 tulokset vahvistivat aikaisempien tutkimusten tuloksia sek\u00e4 havainnollistivat suolistomikrobiston ja metaboliittien v\u00e4list\u00e4 kompleksia suhdetta.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Exposure to excess ionising radiation can cause cellular damage and thus affect organism health. Bank voles (Clethrionomys glareolus) inhabiting the Chornobyl Exclusion Zone (CEZ) may be exposed to elevated levels of radiation due to the presence of radionuclides in the environment following the accident in the nuclear power plant. One of the reported impacts of exposure to high dose of radiation is a change in the composition of the gut microbiota. Whether this change alters the metabolites is not known. For example, many gut microbes could perform similar functions, in which case the alteration of the microbiota may not have a notable effect on the services provided to the host, e.g. metabolite production. Addressing this knowledge gap, bank vole faecal samples were collected from contaminated and uncontaminated areas within the CEZ and uncontaminated areas outside the CEZ and near Kyiv. The microbiota and metabolite composition of all samples was collected as part of the Earth Microbiome 500 project. Briefly, the microbiota were characterised using 16S rRNA v4 amplicon sequencing, with data processed in QIIME2 and analysed using packages within PHYLOSEQ and VEGAN. Metabolite determined using untargeted metabolomics (ultra-high performance liquid chromatography coupled to a mass spectrometer), with peak calling in MZmine and subsequent data analyses in PHYLOSEQ/VEGAN. I found that (1) radiation significantly affected the alpha and beta diversities of gut microbiota, but not the metabolites and (2) the changes in gut bacteria diversity were not readily associated with the changes in metabolite diversity. These results strengthened an earlier understanding that exposure to an increased level of radiation alters the gut microbiota of bank voles. Since exposure radiation affected the gut microbiota diversity, but no association was found between changes in gut microbiota and metabolites, this reinforces a complexity of understanding whether changes in microbiota communities inferred from amplicon sequencing data can be interpreted in term of alterations to key services such as the provision of metabolites.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2024-06-24T07:48:48Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-06-24T07:48:48Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "55", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "gut microbiota", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "metabolites", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Association between microbiota community diversity and metabolite diversity : bank voles (Clethrionomys glareolus) inhabiting the Chornobyl Exclusion Zone as a model", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202406244956", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mets\u00e4myyr\u00e4", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "ionisoiva s\u00e4teily", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microbiome", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "Clethrionomys glareolus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "ionising radiation", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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