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[{"key": "dc.contributor.advisor", "value": "Yl\u00e4nne, Jari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Korkiam\u00e4ki, Riku", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Hakanen, Mikko", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-06-24T07:07:55Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-06-24T07:07:55Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/96102", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Drosophila melanogasterin alkiovaiheen aikana tapahtuvassa soluuntumisessa on mukana useita eri aktiinis\u00e4ikeit\u00e4 yhteenliitt\u00e4vi\u00e4 proteiineja, jotka kontrolloivat aktomyosiinirenkaan muotoa ja kokoa. Filamiinin ortologi cheerio on yksi n\u00e4ist\u00e4 proteiineista. Sen karboksip\u00e4\u00e4ss\u00e4 on mekanosensorinen alue, joka sis\u00e4lt\u00e4\u00e4 kaksi peitetty\u00e4 proteiinin vuorovaikutuspaikkaa. T\u00e4m\u00e4n alueen rooli soluuntumisessa ei ole viel\u00e4 t\u00e4ysin selvill\u00e4. Kokeessa k\u00e4ytettiin kolmea eri k\u00e4rp\u00e4slinjaa, jotka vaihtelivat proteiinien vuorovaikutuspaikkojen saavutettavuuden helppoudessa. Alkion soluuntumista kuvattiin el\u00e4vist\u00e4 alkioista. Konfokaali mikroskoopi pinoja otettiin 3 minuutin v\u00e4lein ja cheerion sijoittumista sek\u00e4 aktomyosiinirengasta soluuntumisen aikana seurattiin GFP-tunnisteen avulla. Linja, jonka proteiinin vuorovaikutusalue oli v\u00e4hiten peitetty, erottui muista linjoista renkaan keh\u00e4n pituuden ja py\u00f6reyden, sek\u00e4 cheerion sijoittumisen suhteen. T\u00e4m\u00e4n linjan renkaiden keh\u00e4n pituus pysyi muita linjoja suurempana koko soluuntumisen ajan, kun taas linjan renkaiden py\u00f6reys laski my\u00f6h\u00e4isen soluuntumisen aikana. Iso osa cheerio proteiineista t\u00e4m\u00e4n linjan soluuntumisessa sijoittuivat pois aktiinimyosiinirenkaiden luota. Tulosten perusteella vaikuttaa silt\u00e4, ett\u00e4 cheerion mekanosensoriselle alueelle on jonkinlainen vaikutus aktomyosiinirenkaaseen, joko suoraan tai ep\u00e4suorasti. Suoraan vaikuttamalla renkaiden muotoon ja supistumiseen tai ep\u00e4suorasti muiden vuorovaikutusten kanssa. Tulokset sopivat yhteen aikaisempiin tutkimustuloksiin, joiden mukaan mekanosensorinen alue on vastuussa filamiinin interaktiosta solukalvon proteiinien kanssa ja n\u00e4in my\u00f6s t\u00e4m\u00e4n kiinnittymisest\u00e4 solukalvoon. T\u00e4t\u00e4 kautta cheerion mekanosensorinen alue n\u00e4ytt\u00e4\u00e4 my\u00f6s kontrolloivan cheerion lokalisaatiota.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Cellularization, a process in the Drosophila melanogaster's embryo stage requires actomyosin polymerization and contraction for membrane invagination and formation of individual cells after multiple mitosis. To accomplish this cellularization utilizes different actin crosslinkers to control the shape and size of actomyosin rings. One of these actin crosslinkers is cheerio, an orthologue of filamin, which contains a mechanosensory region with two masked protein interaction sites in its C-terminal. The role of this region in cellularization is not yet clear. Cellularization from three different fly lineages, which differed in the ease of access to their protein interaction sites, was imaged from living embryos. Confocal microscopy stacks were taken with 3-minute intervals and the localization of cheerio at the actomyosin ring was followed with a GFP tag. The genotype with the least restriction to its protein interaction site separated itself from other genotypes by its perimeter and circularity values and by being localized differently. It obtained larger perimeter length values throughout the cellularization process and decreased in circularity values through the late cellularization. This genotype also had most of its cheerio localized away from the actomyosin rings. These results suggest that the cheerio mechanosensory region affect the actomyosin rings during cellularization, either directly by altering their conformation and contraction, or indirectly by localizing cheerio away from the rings and letting the process complete without the crosslinker\u2019s influence. The results are also in accordance with previous filamin studies suggesting that the mechanosensory region is responsible for the filamins attachment to membrane through transmembrane protein interaction. Based on this the mechanosensory region of cheerio seems to also control the localization of cheerio.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2024-06-24T07:07:54Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-06-24T07:07:55Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "34", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "Actin crosslinker", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Actomyosin", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Filamin", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The cheerio mechanosensory region fine-tunes Drosophila cellularization", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202406244949", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "filamiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solukalvot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aktomyosiini", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aktiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "alkio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "filamins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell membranes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "actomyosin", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "actins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "embryo", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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