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[{"key": "dc.contributor.advisor", "value": "Rautajoki, Kirsi", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Tiihonen, Aliisa", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ihalainen, Janne", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Savola, Noora", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-06-12T06:21:15Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-06-12T06:21:15Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/95767", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Mapping the tumor microenvironment provides essential information about cancer diagnosis, progression, and possible treatments. Glioblastoma is the most common primary malignant brain tumor where tumor heterogeneity together with the tumor microenvironment cause ineffectiveness of treatments and poor prognosis of the disease. For this reason, a comprehensive mapping of the glioblastoma microenvironment and better understanding of the role of immune cells are key to studying the biology of the disease and developing new treatments. The microenvironment of glioblastoma consists of different cancer cell subtypes, different types of neural cells, local and infiltrating immune cells, and vascular endothelial cells and pericytes. The aim of this thesis was to optimize multiplex fluorescent immunohistochemistry staining for fresh frozen brain tumor samples to map the tumor microenvironment. The goal was to create a staining protocol which covers markers to identify the main cell types: the marker CD45 used to quantify immune cell infiltration, the T-cell markers CD3, CD4 and CD8, the macrophage marker CD163, macrophage and dendritic cell marker CD11c, the TMEM119 marker for distinguishing microglia from the macrophages infiltrating the tumor area, the endothelial cell marker CD31 for identifying blood vessels, and the Ki67 marker for evaluating cell proliferation. The durability and behavior of fresh frozen tissue and of the tissue section was tested in different staining phases. After this fresh frozen human glioblastoma samples were used to optimize antibodies and staining order for the final protocol. An optimized staining protocol for fresh frozen samples enables characterization of the tumor microenvironment of brain tumors together with sequencing-based spatial tissue analysis.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Kasvaimen mikroymp\u00e4rist\u00f6n kartoittaminen antaa olennaista tietoa sy\u00f6v\u00e4n diagnoosista, etenemisest\u00e4 sek\u00e4 mahdollisista hoidoista. Glioblastooma on aivojen yleisin pahanlaatuinen primaarikasvain, jonka sis\u00e4inen heterogeenisyys yhdess\u00e4 kasvaimen mikroymp\u00e4rist\u00f6n kanssa vaikuttavat hoitojen tehoon sek\u00e4 taudin huonoon ennusteeseen. T\u00e4st\u00e4 syyst\u00e4 glioblastooman mikroymp\u00e4rist\u00f6n kokonaisvaltainen kartoittaminen sek\u00e4 immuunisolujen roolin parempi ymm\u00e4rt\u00e4minen ovat avainasemassa taudin biologian tutkimisessa sek\u00e4 uusien hoitojen kehitt\u00e4misess\u00e4. Glioblastooman mikroymp\u00e4rist\u00f6 koostuu maligneista astrosytoomasoluista, v\u00e4hemm\u00e4n erilaistuneista sy\u00f6v\u00e4n kantasoluista, erilaisista hermosoluista, paikallisista ja tunkeutuvista immuunisoluista sek\u00e4 verisuonten endoteelisoluista ja perisyyteist\u00e4. T\u00e4m\u00e4n tutkielman tavoitteena oli optimoida fluoresoivaan immunohistokemiaan perustuva multiplex-v\u00e4rj\u00e4ys tuoreille pakastetuille aivokasvainn\u00e4ytteille tuumorimikroymp\u00e4rist\u00f6n kartoittamiseksi. Tavoitteena oli luoda v\u00e4rj\u00e4ysprotokolla kattamaan markkerit t\u00e4rkeimpien solutyyppien kartoittamiseen, joihin kuuluvat esimerkiksi immuunisolujen infiltraation kvantifioimiseen k\u00e4ytett\u00e4v\u00e4 markkeri CD45, T-solumarkkerit CD3, CD4 ja CD8 makrofagimarkkeri CD163, dendriittisolumarkkeri CD11c, TMEM119-markkeri mikroglioiden erottamiseen kasvainalueelle tunkeutuvista makrofageista, endoteelisolumarkkeri CD31, sek\u00e4 solujen lis\u00e4\u00e4ntymisen arviontiin markkeri Ki67. Optimointi aloitettiin hiiren aivon\u00e4ytteill\u00e4, joiden avulla tutkittiin tuoreen pakastetun kudoksen k\u00e4ytt\u00e4ytymist\u00e4 sek\u00e4 kudosleikkeen kest\u00e4vyytt\u00e4 eri v\u00e4rj\u00e4ysvaiheissa. T\u00e4m\u00e4n j\u00e4lkeen siirryttiin k\u00e4ytt\u00e4m\u00e4\u00e4n ihmisper\u00e4isi\u00e4 tuoreita pakastettuja glioblastooman\u00e4ytteit\u00e4 joiden avulla testattiin v\u00e4rj\u00e4yst\u00e4 vasta-ainepareilla ja lopulta koko v\u00e4rj\u00e4ysprotokollaa per\u00e4kk\u00e4isill\u00e4 v\u00e4rj\u00e4yksill\u00e4. Optimoitu v\u00e4rj\u00e4ysprotokolla tuoreille pakastetuille n\u00e4ytteille mahdollistaa aivokasvainten tuumorimikroymp\u00e4rist\u00f6n karakterisoinnin yhdess\u00e4 sekvensointiin perustuvan spatiaalisen kudosanalyysin kanssa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2024-06-12T06:21:15Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-06-12T06:21:15Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "66", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "cancer", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "immunofluorescence", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "tumor microenvironment", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Optimization of cyclic multiplex immunohistochemistry staining for fresh frozen brain tumor samples", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202406124534", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "keskushermosto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aivokasvaimet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sy\u00f6p\u00e4taudit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "glioblastooma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immunohistokemia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "vasta-aineet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "central nervous system", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "brain tumors", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cancerous diseases", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "glioblastoma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immunohistochemistry", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibodies", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities/facilities#autotoc-item-autotoc-2).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat#autotoc-item-autotoc-2.", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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