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[{"key": "dc.contributor.author", "value": "Oksaranta, Emilia", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-05-29T06:27:38Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-05-29T06:27:38Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/95301", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Regular physical activity produces beneficial effects on health in obesity, especially for the vascular system. Three-dimensional imaging (3D) using tissue clearing techniques aids in studying complex structures like vasculature. However, only one preliminary study has examined the 3D skeletal muscle vasculature using similar technique. Since this method has not been extensively studied before, this thesis aimed to develop the method for whole mouse muscles and to investigate the effects of exercise on skeletal muscle arterial network using 3D imaging in normal and overweight mice. Adult C57BL/6J male mice (n=40) were divided into four groups: lean sedentary, lean exercise, obese sedentary and obese exercise. Obesity, hyperglycemia and insulin resistance were induced using a Western diet for 18 weeks. The exercise groups had free access to running wheels for the last 9 weeks within their cages. The entire intervention lasted 18 weeks. Tibialis anterior muscles of mice (n=12, 3/group) were stained with SMA-Cy3 antibody to visualize the arterial network and cleared for 3D vascular structure analysis utilizing light sheet microscopy and Imaris software. Other methods utilized in this study included body composition analysis, glucose tolerance test and maximal running capacity test. Body weight and fat percentage were significantly increased in the obese mice. However, glucose tolerance was impaired only in the obese sedentary group (p<0.0001), demonstrating the metabolic health benefit of exercise in the obese mice. The development of the 3D imaging protocol was successful and visual analysis with Imaris enabled the exploration of the 3D arterial structure. The 3D quantitative analyses revealed no statistically significant differences in arterial characteristics such as area, volume, total length, branch length, diameter, branch level and depth between the groups. 3D visualization of muscles using whole mount staining and iDISCO tissue clearing protocol proved effective in analyzing skeletal muscle arterial vasculature. With this rather small sample size, the results indicate that the arterial network was not affected by exercise and obesity. Further studies using a larger sample size needs to be conducted to evaluate these effects in more detail.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "S\u00e4\u00e4nn\u00f6llinen fyysinen aktiivisuus tarjoaa lukuisia terveyshy\u00f6tyj\u00e4 ylipainoisille, erityisesti verisuoniston kannalta. Kolmiulotteinen kuvantaminen mahdollistaa monimutkaisten rakenteiden, kuten verisuoniston, tutkimisen. Kolmiulotteisen kuvantamisen ja analyysin apuv\u00e4lineen\u00e4 voidaan k\u00e4ytt\u00e4\u00e4 kudosl\u00e4p\u00e4isytekniikkaa, jonka tarkoituksena on v\u00e4hent\u00e4\u00e4 valon sirontaa tekem\u00e4ll\u00e4 n\u00e4ytteest\u00e4 l\u00e4pin\u00e4kyv\u00e4. T\u00e4m\u00e4n tutkielman tavoitteena oli tarkastella liikunnan aiheuttamia vaikutuksia luustolihasten valtimoverkoston rakenteeseen k\u00e4ytt\u00e4en kolmiulotteista kuvantamista normaalipainoisilla ja ylipainoisilla hiirill\u00e4. Aikuiset C57BL/6J uroshiiret (n=40) jaettiin nelj\u00e4\u00e4n ryhm\u00e4\u00e4n: laihat liikkumattomat, laihat liikkuvat, ylipainoiset liikkumattomat ja ylipainoiset liikkuvat. Kokeen kokonaispituus oli 18 viikkoa. Lihavuus, hyperglykemia ja insuliiniresistenssi aiheutettiin koko kokeen ajan kest\u00e4v\u00e4ll\u00e4 korkearasvaisella ruokavaliolla. J\u00e4lkimm\u00e4iset 9 viikkoa liikkuvien ryhmill\u00e4 oli h\u00e4keiss\u00e4\u00e4n vapaa p\u00e4\u00e4sy juoksupy\u00f6rille. Hiirten etummaiset s\u00e4\u00e4rilihakset (n=12, 3/ryhm\u00e4) v\u00e4rj\u00e4ttiin SMA-Cy3-vasta-aineella ja tehtiin l\u00e4pin\u00e4kyv\u00e4ksi 3D verisuonirakenteen analysointia varten. 3D analyysit tehtiin valomikroskooppia ja Imaris-analyysiohjelmaa hy\u00f6dynt\u00e4en. Tutkimuksessa k\u00e4ytettiin my\u00f6s kehonkoostumusmittausta, glukoositoleranssitesti\u00e4 ja maksimaalista juoksutesti\u00e4 kuvaamaan hiirten fysiologisia ominaisuuksia. Ylipainoisilla hiirill\u00e4 kehon paino ja rasvaprosentti kasvoivat merkitt\u00e4v\u00e4sti. Kuitenkin glukoositoleranssi oli heikentynyt vain liikkumattomien ylipainoisten ryhm\u00e4ll\u00e4 (p<0,0001), mik\u00e4 osoittaa liikunnan positiivisen hy\u00f6dyn metabolisen terveyden kannalta ylipainoisilla hiirill\u00e4. 3D-kuvantamisprotokollan kehitys onnistui ja se mahdollisti visuaalisen ja numeraalisen 3D-valtimorakenteen analysoinnin Imaris-ohjelmaa hy\u00f6dynt\u00e4en. Kvantitatiiviset analyysit eiv\u00e4t paljastaneet tilastollisesti merkitsevi\u00e4 eroja valtimoiden pinta-alassa, tilavuudessa, kokonaispituudessa, haaran pituudessa, halkaisijassa, haaran tasoilla eik\u00e4 haarojen syvyydess\u00e4. Lihasten 3D-visualisointi koko kudoksen v\u00e4rj\u00e4yksell\u00e4 ja iDISCO-kudosl\u00e4p\u00e4isytekniikalla osoittautui tehokkaaksi protokollaksi luurankolihaksen verisuoniston analysoinnissa. T\u00e4m\u00e4n tutkimuksen tulosten perusteella liikunta ja lihavuus eiv\u00e4t vaikuta merkitt\u00e4v\u00e4sti valtimoverkoston rakenteeseen. Tulevaisuudessa tarvitaan kuitenkin tutkimusta suuremmalla otoskoolla.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2024-05-29T06:27:38Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-05-29T06:27:38Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "58", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "tissue clearing", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "whole mount staining", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "3D imaging and analysis of skeletal muscle arterial network structure in response to exercise training in normal and overweight mice", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202405294066", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sport and Health Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Liikuntatieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Liikunta- ja terveystieteet", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Sport and Health Sciences", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Liikuntafysiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Exercise Physiology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "5011", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "lihavuus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "lihakset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "valtimot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "verisuonet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fyysinen aktiivisuus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "obesity", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "muscles", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "arteries", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "blood vessels", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "physical activity", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities/facilities#autotoc-item-autotoc-2).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat#autotoc-item-autotoc-2.", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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