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[{"key": "dc.contributor.advisor", "value": "Hulmi, Juha", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "M\u00e4ntyselk\u00e4, Sakari", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Yl\u00e4nne, Jari", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Lahtonen, Suvi", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-05-15T10:09:54Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-05-15T10:09:54Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2024", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/94861", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Lihasmassa on t\u00e4rke\u00e4 ihmisen toimintakyky\u00e4 ja aineenvaihduntaa m\u00e4\u00e4ritt\u00e4v\u00e4 tekij\u00e4. Lihasmassan m\u00e4\u00e4r\u00e4\u00e4n vaikuttaa p\u00e4\u00e4asiassa lihaksissa tapahtuva proteiinisynteesin ja proteiinien hajotuksen suhde. Lihasmassaa ja proteiinisynteesi\u00e4 s\u00e4\u00e4telev\u00e4t mekanismit ovat viel\u00e4 osittain ep\u00e4selv\u00e4t, mutta aineenvaihdunnan muutoksilla ja s\u00e4\u00e4telyll\u00e4 on havaittu olevan merkitt\u00e4v\u00e4 rooli lihassolujen kasvun ja jakautumisen s\u00e4\u00e4telyss\u00e4. Glykolyysin rinnakkaisreitit tarjoavat l\u00e4ht\u00f6materiaaleja elimist\u00f6ss\u00e4 tapahtuviin biosynteeseihin ja n\u00e4ill\u00e4 reiteill\u00e4 voisi olla potentiaalinen merkitys my\u00f6s lihaskoon muutoksiin. T\u00e4m\u00e4n tutkielman tavoitteena oli tutkia seriinisynteesireitin ja etenkin reitin aloittavan fosfoglyseraattidehydrogenaasi (PHGDH)-entsyymin merkityst\u00e4 lihaskoon ja lihassolujen aineenvaihdunnan s\u00e4\u00e4telyss\u00e4. Lis\u00e4ksi tutkimuksessa haluttiin my\u00f6s selvitt\u00e4\u00e4 mahdollisia mekanismeja, joilla PHGDH-entsyymin toiminnan est\u00e4minen v\u00e4hent\u00e4\u00e4 lihassolujen proteiinisynteesi\u00e4. Tutkimukset suoritettiin altistamalla ihmisen prim\u00e4\u00e4rilihassoluja ja hiiren C2C12-lihassoluja PHGDH-inhibiittorille, mink\u00e4 j\u00e4lkeen solujen jakautumista ja myotuubien halkaisijoita tarkasteltiin konfokaalimikroskopian avulla sek\u00e4 proteiinisynteesi\u00e4 ja anabolista signalointia tutkittiin Western blot -menetelm\u00e4ll\u00e4. Tuloksena havaittiin, ett\u00e4 seriinisynteesireitin PHGDH-entsyymin toiminnan est\u00e4minen v\u00e4hensi lihassolujen jakautumista ja pienensi myotuubien kokoa suhteessa kontrolliin. PHGDH-inhibitio johti lihassolujen proteiinisynteesin laskuun, ja mTOR-alavirran proteiineja tutkimalla voitiin p\u00e4\u00e4tell\u00e4, ett\u00e4 proteiinisynteesin lasku voisi mahdollisesti johtua mTORC1:n toiminnan heikkenemisest\u00e4. Seriinisynteesireitill\u00e4 ja siin\u00e4 tapahtuvilla muutoksilla oli siis selke\u00e4 vaikutus lihasmassan ja lihaskoon s\u00e4\u00e4telyss\u00e4 in vitro -olosuhteissa. Glykolyysin rinnakkaisreittien merkityksen ymm\u00e4rt\u00e4minen lihaskoon s\u00e4\u00e4telyss\u00e4 saattaa pitk\u00e4ll\u00e4 t\u00e4ht\u00e4imell\u00e4 hy\u00f6dytt\u00e4\u00e4 uusien hoitomuotojen kehityksess\u00e4 lihasten surkastumisen ehk\u00e4isyss\u00e4 ja hoidossa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2024-05-15T10:09:54Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-05-15T10:09:54Z (GMT). No. of bitstreams: 0\n Previous issue date: 2024", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "25", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "luurankolihas", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mTORC1", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "myoblasti", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "myotuubi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "proteiinisynteesi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Seriinisynteesireitin PHGDH-entsyymin vaikutus lihassolujen kasvuun ja aineenvaihduntaan", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202405153628", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "lihakset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aineenvaihdunta", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities/facilities#autotoc-item-autotoc-2).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat#autotoc-item-autotoc-2.", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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