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[{"key": "dc.contributor.advisor", "value": "Elomaa, Hanna", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Ihalainen, Janne", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Tarkiainen, Vilma", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-03-13T06:41:55Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-03-13T06:41:55Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2023", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/93886", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Colorectal cancer is a global problem and one of the leading causes of cancer-associated deaths. It is diagnosed with standard classification based on the tumour node metastasis classification system provided by the Union for International Cancer Control and the American Joint Committee on Cancer. In many cases, colorectal cancer is detected in advanced stages, and the prognosis is usually less favourable compared to if the cancer had been diagnosed in earlier stages. Although clinicopathological staging predicts the patient\u2019s survival relatively well, some patients would benefit from more precise estimation. Thus, there is a need for finding novel prognostic markers to advise treatment decisions towards personalized medicine. In this thesis, the expression of mucins in colorectal cancer was studied. Mucins are glycoproteins that have essential roles in epithelial tissues, such as cell signalling and the formation of protective barriers. Aberrant expression of some mucins in colorectal cancer is found to be associated with cancer progression. In this work, the expression of five mucins: MUC1, MUC2, MUC4, MUC5AC and MUC6 was analysed. The expression was studied using immunohistochemistry staining. Associations between mucins and clinicopathological factors and colorectal cancer survival were assessed. MUC1 protein was the most prominent factor related to the prognosis, and higher cytoplasmic expression of MUC1 was associated with worse survival in multiple analyses. In addition, higher cytoplasmic expression of MUC4 was associated with worse survival. These results indicate that MUC1 and MUC4 could be considered prognostic markers in colorectal cancer. In addition, Fourier transform infrared (FTIR) spectroscopy was used to determine whether the normal colon and cancerous tissue could be distinguished based on spectra. Spectroscopic analysis was conducted for normal colon, mucinous colorectal cancer, and colorectal cancer. Some differences in the spectra between tissue types were detected. This study demonstrated that FTIR spectroscopy could be potentially used for cancer research. However, as a diagnostic method, FTIR spectroscopy needs further validation.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Suolistosy\u00f6p\u00e4 eli paksu- ja per\u00e4suolen sy\u00f6p\u00e4 on maailmanlaajuinen taakka terveydenhuollolle ja yksi suurimmista sy\u00f6p\u00e4kuolemien aiheuttajista. Suolistosy\u00f6v\u00e4t diagnosoidaan usein jo edenneiss\u00e4 sy\u00f6v\u00e4n vaiheissa ja ennuste on merkitt\u00e4v\u00e4sti huonompi, kuin jos sy\u00f6p\u00e4 havaittaisiin varhaisessa vaiheessa. Sy\u00f6p\u00e4 diagnosoidaan tiettyjen standardien perusteella ja usein potilaat samankaltaisilla diagnooseilla hy\u00f6tyisiv\u00e4t erilaisista hoitomuodoista. N\u00e4m\u00e4 havainnot perustelevat tarpeen l\u00f6yt\u00e4\u00e4 uusia ennustemerkkej\u00e4, joita voitaisiin hy\u00f6dynt\u00e4\u00e4 suolistosy\u00f6p\u00e4potilaan ennusteen ja hoidon yksil\u00f6llisest\u00e4misess\u00e4. T\u00e4ss\u00e4 ty\u00f6ss\u00e4 tutkittiin musiinien merkityst\u00e4 suolistosy\u00f6viss\u00e4, sill\u00e4 joidenkin niist\u00e4 on havaittu ilmenev\u00e4n suolistosy\u00f6viss\u00e4 poikkeavasti ja olevan yhteydess\u00e4 sy\u00f6v\u00e4n kehitykseen. Musiinit ovat glykoproteiineja, joilla on useita teht\u00e4vi\u00e4 normaalissa suolistossa. Kuten solunsignaloinnissa sek\u00e4 suojaavina tekij\u00f6in\u00e4 epiteelisolujen pinnalla. Ty\u00f6ss\u00e4 tutkittiin MUC1, MUC2, MUC4, MUC5AC ja MUC6 proteiinien yhteytt\u00e4 ennusteeseen ja kliinispatologisiin muuttujiin.\nMUC-proteiinien ilmenemist\u00e4 analysoitiin v\u00e4rj\u00e4\u00e4m\u00e4ll\u00e4 sy\u00f6v\u00e4n kudosn\u00e4ytteit\u00e4 immunohistokemiallisesti. MUC1 proteiinin korkean ilmenemisen solulimassa havaittiin olevan yhteydess\u00e4 huonompaan ennusteeseen useissa eri analyyseissa. My\u00f6s MUC4 proteiinin korkean ilmenemisen solulimassa havaittiin olevan yhteydess\u00e4 huonompaan ennusteen. N\u00e4m\u00e4 tulokset viittaavat MUC1 ja MUC4 proteiinien ilmentymisen olevan mahdollisia ennustetekij\u00f6it\u00e4, joita voitaisiin hy\u00f6dynt\u00e4\u00e4 lis\u00e4n\u00e4 suolistosy\u00f6p\u00e4potilaan ennusteen arvioinnissa. N\u00e4iden tutkimusten lis\u00e4ksi Fourier-muunnos infrapuna (FTIR) spektroskopiaa tutkittiin mahdollisena ty\u00f6kaluna sy\u00f6p\u00e4tutkimuksessa. Tavoitteena oli selvitt\u00e4\u00e4, voidaanko FTIR spektroskopiaa hy\u00f6dynt\u00e4en erottaa sy\u00f6p\u00e4kudos normaalista kudoksesta. Spektroskooppinen analyysi tehtiin normaalin, suolistosy\u00f6v\u00e4n ja musinoottisen sy\u00f6p\u00e4kudoksen v\u00e4lill\u00e4. Eroja eri kudostyyppien spektreiss\u00e4 pystyttiin havainnoimaan, mik\u00e4 viittaa FTIR spektroskopian hy\u00f6dyllisyyteen sy\u00f6p\u00e4diagnostiikassa. T\u00e4m\u00e4n tutkimuksen tulokset ovat kuitenkin vain osoitus potentiaalisesta ty\u00f6kalusta sy\u00f6p\u00e4diagnostiikassa ja menetelm\u00e4 vaatii viel\u00e4 runsaasti lis\u00e4\u00e4 tutkimuksia.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2024-03-13T06:41:55Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-03-13T06:41:55Z (GMT). No. of bitstreams: 0\n Previous issue date: 2023", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "53", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "colorectal carcinoma", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Fourier transform infrared spectroscopy", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MUC1", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MUC2", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MUC4", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MUC5AC", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MUC6", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mucus", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "prognosis", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Analysis of Mucin glycoprotein expression in colorectal cancer", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202403132351", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.collaborator", "value": "public", "language": "", "element": "contractresearch", "qualifier": "collaborator", "schema": "yvv"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "yvv.contractresearch.initiative", "value": "student", "language": "", "element": "contractresearch", "qualifier": "initiative", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": null, "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immunohistokemia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sy\u00f6p\u00e4taudit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "biomarkkerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suolistosy\u00f6v\u00e4t", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immunohistochemistry", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cancerous diseases", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "biomarkers", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bowel cancer", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/en/workspaces/facilities/facilities#autotoc-item-autotoc-2).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/fi/tyoskentelytilat/laitteet-ja-tilat#autotoc-item-autotoc-2.", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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