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[{"key": "dc.contributor.advisor", "value": "Yl\u00e4nne, Jari", "language": null, "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Siipilehto, Panu", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2024-01-29T07:42:45Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2024-01-29T07:42:45Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2023", "language": null, "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/93097", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Cells can regulate cellular processes by sensing mechanical forces in a process known as mechanotransduction. This can be achieved with cytoskeletal proteins such as actin crosslinking protein filamin. Fruit fly filamin is known as Cheerio (Cher). In this study, I focused on actomyosin regulations during the cellularization phase of Drosophila early embryogenesis. Specifically, I studied filamin, myosin regulatory light chain protein called Spaghetti squash (Sqh), and Death-associated kinase-related (Drak). Prior research has shown that filamin and Sqh colocalize during cellularization. Additionally, Drak binds to filamin, and both Drak and filamin are involved in the regulation of cellularization. Drak also phosphorylates Sqh, but its localization during Drosophila cellularization is unknown. I used Drosophila melanogaster lines that expressed Green fluorescent protein (GFP) or mCherry red fluorescent protein fusions of Cher, Drak, and Sqh. Early embryos were time-lapse imaged with confocal microscopy and analyzed with ImageJ software. Pearson\u2019s Correlation Coefficient (PCC) was calculated for selected region-of-interests. The main results showed that a proportion of Drak localizes to actin front together with Cher. Cher and Sqh PCC increased as cellularization progresses. In contrast, Drak and Cher colocalization remained similar throughout. Drak also localized near cellular cortex between nuclear divisions and throughout the cellularization process. My results suggest that Drak may have a role in both the cortical region and in the cellularization front. These results provide further knowledge for characterizing the mechanically regulated signaling pathways of filamin.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Solut s\u00e4\u00e4telev\u00e4t monia solun prosesseja aistimalla mekaanisia voimia ja muuttamalla ne biokemiallisiksi signaaleiksi. Yksi t\u00e4h\u00e4n osallistuvista proteiineista on aktiinis\u00e4ikeit\u00e4 yhteenliitt\u00e4v\u00e4 filamiini. Banaanik\u00e4rp\u00e4sen filamiini on nimelt\u00e4\u00e4n Cheerio (Cher). Tutkimuksessa keskityin aktomyosiinin s\u00e4\u00e4telyyn Drosophila-k\u00e4rp\u00e4sen alkion varhaisen solujen muodostumisen aikana. Erityisesti tarkastelin filamiinin, myosiinin s\u00e4\u00e4telyalayksikk\u00f6\u00e4 nimelt\u00e4 Spaghetti squash (Sqh) ja solukuolemaan liittyv\u00e4\u00e4 proteiinikinaasin (Drak) lokalisaatioita. Aiemmat tutkimukset ovat osoittaneet, ett\u00e4 filamiini yhteislokalisoituu Sqh:n kanssa varhaisen solujen muodostumisen aikana. Lis\u00e4ksi Drak sitoutuu filamiiniin, ja sek\u00e4 Drak ett\u00e4 filamiini osallistuvat varhaisen solujen muodostumisen s\u00e4\u00e4telyyn. Drak pystyy my\u00f6s s\u00e4\u00e4telem\u00e4\u00e4n Sqh:t\u00e4 fosforylaation kautta, mutta sen lokalisaatio ei ole tiedossa. K\u00e4ytin tutkimuksessa Drosophila melanogaster -linjoja, jotka ilmaisivat vihre\u00e4t\u00e4 fluoresoivaa proteiinia (GFP) tai punaista fluoresoivaa proteiinia (mCherry) Cher:n, Drak:n ja Sqh:n proteiiniyhdistein\u00e4. Varhaiset k\u00e4rp\u00e4salkiot kuvattiin aikaseurannalla konfokaalimikroskoopilla ja analysoitiin ImageJ -ohjelmalla. Pearsonin kolokalisaatiokerroin (PCC) laskettiin valituille kohdealueille. Tutkimuksen p\u00e4\u00e4tulos osoitti, ett\u00e4 Drak lokalisoituu osittain aktiinifronttiin Cher:n kanssa. Cher:n ja Sqh:n PCC kasvaa aktiinifrontin edetess\u00e4, kun taas Drak:n ja Cher:n v\u00e4lill\u00e4 se pysyy samana. Drak-signaali lokalisoitui my\u00f6s l\u00e4hell\u00e4 solukerrosrakennetta tumanjakautumisten v\u00e4lill\u00e4 ja solujen muodostumisen aikana. Tutkimukseni tulokset viittaavat siihen, ett\u00e4 Drak-proteiinilla voi olla rooli solukuoren alueella sek\u00e4 aktiinifrontissa. N\u00e4m\u00e4 tulokset tarjoavat my\u00f6s lis\u00e4tietoa filamiinin mekaanisesti s\u00e4\u00e4dellyn signaloinnin karakterisoinnista.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2024-01-29T07:42:45Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2024-01-29T07:42:45Z (GMT). No. of bitstreams: 0\n Previous issue date: 2023", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "54", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "actin front", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "MLCK", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "cellularization", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "live tissue imaging", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Death-associated kinase-related (Drak) localization in Drosophila during early embryogenesis", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202401291584", "language": null, "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": null, "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": null, "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "filamiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solubiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fosforylaatio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "myosiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "alkionkehitys", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikroskopia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aktomyosiini", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "filamins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell biology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "phosphorylation", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "myosins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "embryogenesis", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microscopy", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "actomyosin", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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