| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Vehni\u00e4inen, Eeva-Riikka", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Leinonen, Anni", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2023-12-08T06:43:42Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2023-12-08T06:43:42Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2023", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/92225", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Fenantreeni (PHE) ja fluoranteeni (FLA), kaksi polysyklist\u00e4 aromaattista hiilivety-yhdistett\u00e4 (PAH), ovat yleisesti vesist\u00f6iss\u00e4 esiintyvi\u00e4 vierasaineita ja myrkyllisi\u00e4 kalojen varhaisille kehitysvaiheille. T\u00e4ss\u00e4 tutkimuksessa tarkasteltiin v\u00e4hemm\u00e4n tunnettua ilmi\u00f6t\u00e4, jossa n\u00e4ille yhdisteille altistuneilla kirjolohen alkiopoikasilla suu ei sulkeudu yht\u00e4 hyvin kuin kontrollipoikasilla, sek\u00e4 muutoksen yhteytt\u00e4 p\u00e4\u00e4n alueen ruston kehitykseen. Poikasia altistettiin PHE:lle ja FLA:lle eri pitoisuuksissa, sek\u00e4 reteenille (RET), PAH-yhdisteelle, jonka tiedet\u00e4\u00e4n aiheuttavan p\u00e4\u00e4nalueen ep\u00e4muodostumia. Poikasten leuan liikett\u00e4 videoitiin ja pys\u00e4ytyskuvien avulla mitattiin arvo kohdalle, jossa suu oli pienimmill\u00e4\u00e4n (MGmin). Ruston tarkastelua varten poikasia s\u00e4il\u00f6ttiin fiksatiiviin ja rustov\u00e4rj\u00e4ttiin. Tulosten perusteella yhden p\u00e4iv\u00e4n j\u00e4lkeen MGmin-arvon keskiarvo oli kontrollia korkeampi altistuksissa 100 ja 200 \u03bcg/l FLA, sek\u00e4 180 \u03bcg/l PHE. Kun poikasia pidettiin puhtaassa vedess\u00e4 seuraavat viisi p\u00e4iv\u00e4\u00e4, tilastollisesti merkitsev\u00e4t erot n\u00e4iden k\u00e4sittelyiden ja kontrollin v\u00e4lill\u00e4 katosivat. Lateraalisessa tarkastelussa p\u00e4\u00e4n rustossa ei l\u00f6ydetty selkeit\u00e4 muutoksia kuusi p\u00e4iv\u00e4\u00e4 180 \u03bcg/l PHE:lle tai 200 \u03bcg/l FLA:lle (vastaavasti 1010 ja 990 nM) altistetuilla poikasilla, mutta 320 \u03bcg/l (1370 nM) reteeni\u00e4 haittasi p\u00e4\u00e4n ruston kehityst\u00e4, mm. lyhent\u00e4en alaleuan Meckelin ruston pituutta suhteessa poikasen pituuteen. Kun leukojen minimiet\u00e4isyys normalisoitiin rustodatalla, my\u00f6s kuusi p\u00e4iv\u00e4\u00e4 320 \u03bcg/l reteeni\u00e4 vaikutti negatiivisesti suun sulkeutumiseen, tosin v\u00e4hemm\u00e4n kuin 180 \u03bcg/l PHE tai 200 \u03bcg/l FLA, joista fluoranteenin vaikutus oli edelleen vahvempi. N\u00e4iden tulosten perusteella vastakuoriutuneet kirjolohenpoikaset voivat toipua suun sulkeutumisen h\u00e4iri\u00f6st\u00e4, joka saatetaan havaita lyhyen, mutta suurikonsentraatioisen, fluoranteeni- tai fenantreeni- altistuksen j\u00e4lkeen. H\u00e4iri\u00f6 ei n\u00e4yt\u00e4 johtuvan reteenille ominaisesta p\u00e4\u00e4n ruston kehitysh\u00e4iri\u00f6st\u00e4, ja yhdess\u00e4 olemassa olevan kirjallisuuden kanssa tulokset viittaavat siihen, ett\u00e4 fluoranteeni ja fenantreeni vaikuttavat todenn\u00e4k\u00f6isesti suoraan leuan toimintaan.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "The polycyclic aromatic hydrocarbons (PAHs) phenanthrene (PHE) and fluoranthene (FLA) are two common contaminants in aquatic environments and are toxic to early life stages of fish. In this MSc research project, the aim was to study a less-known effect, an impairment of mouth closure, and its relation to cartilage structure in rainbow trout yolk sac larvae. Larvae were exposed to several concentrations of PHE and FLA, and to retene (RET), a PAH-compound known to produce craniofacial malformations. Jaw movements were filmed and the smallest observable mouth gape measured from still images. For cartilage examination, some larvae were fixed and the cartilage structures stained using Alcian blue. According to results, one day of exposure increased the average of the mouth gape minimum (MGmin) of groups exposed to 100 or 200 \u03bcg/l FLA or 180 \u03bcg/l PHE. After five days of recovery in clean water, statistically significant differences between these groups and control were no longer present. Cartilage analysis revealed no clear changes in the lateral chondrocrania of individuals exposed to 180 \u03bcg/l PHE or 200 \u03bcg/l FLA (1010 and 990 nM respectively) for six days compared to control. In turn, exposure to 320 \u03bcg/l (1370 nM) of retene was found to impede chondrocranium development, which included the reduction of the Meckel\u2019s cartilage of the lower jaw compared to larvae length. The cartilage normalised jaw distance values (CNJD) of the MGmin obtained for six days of exposure showed that 320 \u03bcg/l RET also negatively affected mouth closure, although less than 180 \u03bcg/l PHE or 200 \u03bcg/l FLA, from which FLA remained more potent. These results indicate that freshly-hatched rainbow trout larvae may recover from a jaw-closure impediment observed after a brief exposure to high concentrations of PHE or FLA, and that this impediment is not due to a retene-like disruption of chondrocranium development. Based on these findings and existing literature, it seems likely that fluoranthene and phenanthrene may act directly on jaw function.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2023-12-08T06:43:42Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2023-12-08T06:43:42Z (GMT). No. of bitstreams: 0\n Previous issue date: 2023", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "90", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "chondrocranium", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "closure", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "craniofacial", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "development", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "fluoranthene", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Meckel\u2019s", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Oncorhynchus mykiss", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "phenanthrene", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "retene", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "An unclosing jaw as a symptom of PAH exposure in rainbow trout yolk sac larvae", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202312088224", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ymp\u00e4rist\u00f6tiede", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Environmental science", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": null, "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "40151", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "rusto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suu", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kirjolohi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kalat", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aromaattiset hiilivedyt", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cartilage", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mouth", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "rainbow trout", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fishes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aromatic hydrocarbons", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
|