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[{"key": "dc.contributor.advisor", "value": "Ihalainen, Janne", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Nissinen, Riitta", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Ketola, Katri", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2023-10-26T06:03:09Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2023-10-26T06:03:09Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2023", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/90668", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Hapellisissa oloissa happea tuottamattomat fotosynteettiset (AAP-) bakteerit saavat kemiallista energiaa hy\u00f6dynt\u00e4m\u00e4ll\u00e4 valoa happea tuottamattomassa fotosynteesiss\u00e4. Hapellinen ymp\u00e4rist\u00f6 on tarpeellinen bakteeriklorofyllien ja karotenoidien valmistukseen, jotka absorboivat valoa valonker\u00e4yskomplekseissa, mist\u00e4 valon viritt\u00e4m\u00e4 energia siirret\u00e4\u00e4n reaktiokeskuksiin eksitaatioenergiansiirto-prosessilla. Bakteeriklorofylli absorboi valoa infrapuna-alueelta ja karotenoidit 400\u2013550 nm alueelta. Karotenoidit voivat siirt\u00e4\u00e4 viritysenergian bakteeriklorofylleille. Toisaalta, karotenoidit suojelevat komplekseja reaktiiviselta hapelta poistamalla ylim\u00e4\u00e4r\u00e4isen viritysenergian. AAP-bakteereja sek\u00e4 niiden pigmenttien tutkimus on v\u00e4h\u00e4ist\u00e4, t\u00e4m\u00e4 tutkimus kehitt\u00e4\u00e4 menetelm\u00e4\u00e4 pigmenttien analysointiin. Pigmentit eluoitiin Sphingomonas glacialis -bakteereista. Identifiointi tehtiin korkean erottelykyvyn nestekromatografian retentioajoilla sek\u00e4 absorptiospektreill\u00e4. Fotosynteettisten pigmenttien kartoitus tehtiin fluoresenssieksitaatiolla. Valo-olosuhteiden muutokset vaikuttivat pigmentteihin ja runsain kasvu tapahtui pime\u00e4ss\u00e4. Pigmenttej\u00e4 l\u00f6ytyi runsaasti, mutta yksik\u00e4\u00e4n ei ollut fotosynteettinen. Pigmenttien analysointimenetelm\u00e4 on toimiva ja k\u00e4ytett\u00e4viss\u00e4 muihin AAP-bakteereihin, mutta karotenoidien tunnistamisessa on viel\u00e4 k\u00e4yt\u00e4nn\u00f6n haasteita.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Aerobic anoxygenic photosynthetic (AAP) bacteria gain chemical energy from light through non-oxygen producing photosynthesis. The presence of the oxygen is required to produce pigments, bacteriochlorophyll a (BChla a) and carotenoids. During photosynthesis pigment constituting light harvesting (LH) complexes gather absorbed light and transfer it to reaction centre (RC) as excitation energy with process called singlet state energy transfer. BChl a is an essential pigment to both the LH complex and RC and its main light absorption area is in near infrared area. Photosynthetic carotenoids absorb light from 400 to 550 nm area and transfer it to the BChls. Carotenoids can act as photoprotectors and quench the excess light energy before it creates high energetic singlet oxygen, that is detrimental to BChl synthesis. The research of AAP bacteria, especially their pigment content, is scarce. This thesis works to find possible method for the pigment analysis and identification. The pigments were extracted from Sphingomonas glacialis and identified with high performance liquid chromatography retention times and absorption spectra. The presence of photosynthetic carotenoids was studied with fluorescence excitation. The light exposure examination addressed effect of light changes to the pigment composition. Pigment synthesis takes place in the darkness. Photosynthetic carotenoids were not present in Sphingomonas glacialis, but multiple carotenoids were found. Identification of the pigments is challenging but the method for pigment analysis is functional and can be utilized in other carotenoid containing AAP bacteria.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2023-10-26T06:03:09Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2023-10-26T06:03:09Z (GMT). No. of bitstreams: 0\n Previous issue date: 2023", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "73", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "BChl a", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "HPLC", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "carotenoids", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "fluorescence excitation", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "singlet state energy transfer", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "anoxygenic photosynthesis", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "anoxygenic photosynthetic bacteria", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Pigment analysis in Sphingomonas glacialis strain S2U11 and its light exposure effect", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202310266697", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": null, "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "yhteytt\u00e4minen", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "karotenoidit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "valotus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "pigmentit (v\u00e4rijauheet)", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fluoresenssi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "imeytyminen", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "photosynthesis", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "carotenoids", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bacteria", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "exposure to light", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "pigments", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fluorescence", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "absorption", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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