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[{"key": "dc.contributor.advisor", "value": "Sundberg, Lotta-Riina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Karvonen, Kati", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Auramo, Jenni", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2023-08-25T05:37:41Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2023-08-25T05:37:41Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2023", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/88683", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "In vitro -solumallit ovat t\u00e4rkeit\u00e4 ty\u00f6kaluja is\u00e4nt\u00e4-patogeeni-vuorovaikutuksen ja mikrobien k\u00e4ytt\u00e4ytymisen tutkimuksessa, ja niit\u00e4 tarvitaan erityisesti\nbakteeritutkimuksessa, sill\u00e4 nis\u00e4k\u00e4ssolumallit vastaavat paremmin in vivo -ymp\u00e4rist\u00f6\u00e4 kuin pelkk\u00e4 bakteeriviljely. Lukuisat virukset ja bakteerit, kuten enterovirukset ja Pseudomonas aeruginosa -bakteeri, aiheuttavat infektioita, joita vastaan ei ole parannuskeinoa, tai infektioiden hoitaminen on vaikeaa. Etenkin Pseudomonas aeruginosa -bakteerin tutkiminen in vitro -solumalleissa on t\u00e4rke\u00e4\u00e4, sill\u00e4 bakteeri on vastustuskykyinen useille antibiooteille ja aiheuttaa sairaalaper\u00e4isi\u00e4 infektioita. T\u00e4m\u00e4n tutkimuksen tavoitteena oli perustaa ja validoida uusi HT-29 solumalli ja sen erilaistamisprotokolla tutkimusk\u00e4ytt\u00f6\u00f6n Jyv\u00e4skyl\u00e4n yliopistolle. Hypoteesina oli, ett\u00e4 t\u00e4m\u00e4 solumalli voidaan erilaistaa limaa tuottaviksi soluiksi, joita voidaan monipuolisesti k\u00e4ytt\u00e4\u00e4 jatkossa\ninfektiomallina. Solut erilaistettiin limaa tuottaviksi soluiksi kasvattamalla niit\u00e4 kahdeksan p\u00e4iv\u00e4\u00e4 topografisella pinnalla. Erilaistuneet solut v\u00e4rj\u00e4ttiin Alcian blue -v\u00e4rill\u00e4 ja testattiin MUC2, villiini ja lysotsyymi vasta-aineilla erilaistumisen ja liman tuoton toteamiseksi. Lis\u00e4ksi erilaistuneet ja erilaistumattomat HT-29 solut infektoitiin coxsackie A9 enteroviruksella, coxsackie B3 enteroviruksella, OC43 koronaviruksella, Pseudomonas aeruginosa -bakteerin kannoilla PA14 ja 573, Salmonella enterica -bakteerin typhimurium -serotyypill\u00e4, Streptococcus mutans -bakteerilla ja Acinetobacter baumannii -bakteerilla, jotta n\u00e4hd\u00e4\u00e4n mitk\u00e4 patogeeneist\u00e4 kykenev\u00e4t infektoimaan n\u00e4it\u00e4 soluja ja mink\u00e4 patogeenien\ntutkimiseen t\u00e4t\u00e4 solulinjaa voidaan tulevaisuudessa k\u00e4ytt\u00e4\u00e4. Vasta-ainetestit eiv\u00e4t kunnolla osoittaneet erilaistumisen onnistumista, mutta Alcian blue -v\u00e4rj\u00e4ys osoitti. Testatuista patogeeneist\u00e4 enterovirukset ja molemmat Pseudomonas aeruginosa -kannat infektoivat soluja ja niit\u00e4 voi tutkia HT-29 solumallissa jatkossakin. Salmonella enterica bakteerin typhimurium -serotyyppi\ninfektoi liev\u00e4sti molempia soluja ja Streptococcus mutans liev\u00e4sti vain erilaistumattomia soluja. Acinetobacter baumannii ei ollut infektiokykyinen. Erilaistettujen limaa tuottavien solujen k\u00e4ytt\u00f6 laajentaa tutkimusmahdollisuuksia. Tutkimus liman ominaisuuksista patogeenej\u00e4 ja infektioita vastaan antaa arvokasta tietoa limakalvojen immuunivasteesta ja auttaa ymm\u00e4rt\u00e4m\u00e4\u00e4n infektion eri vaiheita ja kehitt\u00e4m\u00e4\u00e4n uusia l\u00e4\u00e4kkeit\u00e4\npatogeenej\u00e4 vastaan.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Knowledge of host-pathogen interaction is important for understanding different microbes and their behaviour. In vitro cell models are important tools in research to examine host-pathogen interaction and the behaviour of pathogens and they are especially needed with bacterial research, because mammalian cell models resemble the in vivo conditions better than traditional bacterial culture.\nThere are several viruses and bacteria, for example enteroviruses and Pseudomonas aeruginosa bacterium, that cause infections towards which there is no cure, or the infections are difficult to treat. Especially studies of the properties of Pseudomonas aeruginosa bacterium in in vitro cell models are needed because the bacterium is resistant to various antibiotics and causes hospital acquired infections. The aim of this study was to establish and validate a novel HT-29 cell model and its differentiation protocol for research use at the University of Jyv\u00e4skyl\u00e4. The hypothesis was that this cell line can be differentiated into mucus producing cells which can be of versatile use in the future. The cells were cultured in a specific topographic surface for eight days to differentiate them into mucusproducing cells. The differentiated cells were stained with Alcian blue stain and tested with MUC2, villin, and lysozyme antibodies to verify their differentiation and mucus production. Additionally, the differentiated and undifferentiated HT29 cells were infected with coxsackievirus A9, coxsackievirus B3, coronavirus OC43, Pseudomonas aeruginosa strains PA14 and 573, Salmonella enterica serovar typhimurium, Streptococcus mutans and Acinetobacter baumannii to discover which of the microbes infect the cells and thus, could be utilised for research in the future. The antibody test results did not fully confirm successful differentiation\nof the cells, but Alcian blue staining did. From the tested pathogens, enteroviruses and both Pseudomonas aeruginosa strains infected the cells and can be used as an infection model in the future with these cells. Salmonella enterica serovar typhimurium infected both cells mildly, while Streptococcus mutans infected mildly only the undifferentiated cells. Acinetobacter baumannii was not\ninfective. The use of differentiated mucus producing cells expands the future research possibilities. Studying the functions of the mucus against pathogens and infections gives valuable information about the immune response of the mucous membranes and can help in understanding the different phases of infections and in developing new medical approaches against pathogens.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2023-08-25T05:37:41Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2023-08-25T05:37:41Z (GMT). No. of bitstreams: 0\n Previous issue date: 2023", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "53", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "host-pathogen interaction", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "in vitro", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mucus", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Validation of HT-29 cell differentiation protocol and the use of HT-29 cell model as an infection model", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202308254773", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": null, "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solumallit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infektiot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "taudinaiheuttajat", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "soluviljely", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "enterovirukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virukset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "Acinetobacter baumannii", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell models", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bacteria", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infections", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "pathogens", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell culture", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "enteroviruses", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "viruses", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "Acinetobacter baumannii", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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