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[{"key": "dc.contributor.advisor", "value": "Kronholm, Ilkka", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Villalba de la Pe\u00f1a, Mariana", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Lehtonen, Suvi-Maaria", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2023-07-10T04:44:24Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2023-07-10T04:44:24Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2023", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/88325", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Ribosomaalinen DNA (rDNA) koodaa rRNA:ta, joka on ribosomien t\u00e4rke\u00e4 \nrakenneosa. Eukaryooteilla rDNA esiintyy lukuisien identtisten toistojaksojen \nsarjoina. rDNA:n toistojaksojen tiedet\u00e4\u00e4n olevan yksi genomin ep\u00e4vakaimmista \nalueista, ja niiss\u00e4 esiintyy paljon kopiolukum\u00e4\u00e4r\u00e4n vaihtelua. Vaikka vaihtelun \nmekanismeista on tehty tutkimusta, syit\u00e4 runsaalle kopiom\u00e4\u00e4r\u00e4lle ja sen \nmuutosalttiudelle ei t\u00e4ysin tunneta, On esitetty, ett\u00e4 se saattaa mahdollistaa \nlokuksen pienenemisen tai laajenemisen my\u00f6t\u00e4 paremman adaptaation muuttuviin \nolosuhteisiin. T\u00e4ss\u00e4 ty\u00f6ss\u00e4 tutkittiin Neurospora crassa -sienen rDNA:n \nkopiolukum\u00e4\u00e4r\u00e4n spontaania mutaatiovauhtia mutaatioakkumulaatiokokeen \navulla ja estimoitiin valinnan vaikutusta mutaatiovauhtiin. Tutkimuksessa \nk\u00e4ytettiin mutaatioakkumulaatiokantoja ja luonnonkantoja. rDNA:n \nkopiolukum\u00e4\u00e4r\u00e4 analysoitiin droplet digital PCR-menetelm\u00e4ll\u00e4. rDNA-mutaatioita \nhavaittiin useissa linjoissa, ja mutaatiovauhdiksi saatiin 18S rDNA:lle 1.10 [0.64, \n1.72] x 10-5 ja 26S rDNA:lle 1.30 [0.09, 1.95] x 10-5 mutaatiota / mitoosi, mik\u00e4 on \nmelko korkea mutaatiovauhti. Deleetioita tapahtui huomattavasti enemm\u00e4n kuin \ninsertioita, mik\u00e4 viittaa siihen, ett\u00e4 mutaatiopaine pienent\u00e4\u00e4 rDNA:n \nkopiolukum\u00e4\u00e4r\u00e4\u00e4 silloin kun muut mekanismit eiv\u00e4t vaikuta siihen. Koska \nmutaatioita tapahtui melko v\u00e4h\u00e4n, mutaatiomallia ei pystytty muodostamaan.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Ribosomal DNA (rDNA) encodes ribosomal RNA, which is an essential structure of \nribosomes. In eukaryotes rDNA loci contain multiple identical tandem repeat \nsequences and are susceptible to copy number variation (CNV). Although the \nmechanisms of copy number increase and decrease have been studied, the reasons \nunderlying CNV are not yet completely understood. It has been suggested that \nenvironmental factors may change the copy number, and therefore it may be one of \nthe mechanisms that helps the organism to adapt to changes in the environment. In \nthis study, spontaneous mutation rate of 18S and 26S rDNA copy number of \nNeurospora crassa was studied using droplet digital PCR. N. crassa lines from a \nmutation accumulation experiment and from natural populations were used. The \nmutation accumulation lines had been cultured for 40 generations in a way that \nminimized the effect of selection. Mutation rate was estimated by comparing rDNA \ncopy numbers between ancestors and different generations. This information was \nthen used to estimate how selection affects mutation rate. The mutation rate in 18S rDNA was 1.10 [0.64, 1.72] x 10-5 and in 26S rDNA 1.30 [0.09, 1.95] x 10-5 mutations per mitosis, which is a relatively high mutation rate. There were significantly more deletions than insertions, suggesting that mutation pressure reduces rDNA copy number when other mechanisms are not involved. However, there were not enough mutations to form a mutation model.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2023-07-10T04:44:24Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2023-07-10T04:44:24Z (GMT). No. of bitstreams: 0\n Previous issue date: 2023", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "38", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "copy number variation", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "effective population size", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mutation accumulation", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "mutation rate", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "rDNA", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Mutation rate of ribosomal DNA copy number in Neurospora crassa", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202307104452", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": null, "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mutaatiot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "DNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geenit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solubiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geneettinen muuntelu", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "populaatiogenetiikka", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mutations", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "DNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "genes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell biology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "genetic variation", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "population genetics", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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