Effects of culture mode and CO2 supply on the efficiency of Haematococcus pluvialis cultivation in recirculating aquaculture system’s wastewater

Haematococcus pluvialis is a microalga that could be grown using wastewater (WW) from recirculating aquaculture systems (RAS) and by growing the alga in WW it is possible to trap the dissolved nutrients which otherwise would end up in the environment. Improvements in sustainability, resource effi...

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Main Author: Rakib, Mazharul
Other Authors: Matemaattis-luonnontieteellinen tiedekunta, Faculty of Sciences, Bio- ja ympäristötieteiden laitos, Department of Biological and Environmental Science, Jyväskylän yliopisto, University of Jyväskylä
Format: Master's thesis
Language:eng
Published: 2023
Subjects:
Online Access: https://jyx.jyu.fi/handle/123456789/88175
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author Rakib, Mazharul
author2 Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_facet Rakib, Mazharul Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä Rakib, Mazharul Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_sort Rakib, Mazharul
datasource_str_mv jyx
description Haematococcus pluvialis is a microalga that could be grown using wastewater (WW) from recirculating aquaculture systems (RAS) and by growing the alga in WW it is possible to trap the dissolved nutrients which otherwise would end up in the environment. Improvements in sustainability, resource efficiency, and the generation of a valuable carotenoid pigment (astaxanthin) may all result from converting wastewater nutrients into H. pluvialis biomass in RAS. In this master's thesis, I have done two experiments. In the first one, I examined the suitability of the culture mode by comparing the growth of H. pluvialis for 17 days in RAS WW under batch (input all the culture medium once) and fed-batch (input culture medium gradually) culture modes. In the second experiment of 15 days, I examined the algal growth and pH of the medium for 8 days until the microalga reach the stationary phase, nitrate removal (15 days), and astaxanthin concentration (on day 15) in Modified Wright's Cryptophyte (MWC) medium with batch culture mode by supplying air either from the RAS trickling filter where CO2 concentration was 964± 16.38 ppm or room air where CO2 was 451± 1.77 ppm. In the 1st experiment, the specific growth rate (SGR) (0.20±0.014 d-1) of the microalga culturing in batch culture mode was significantly higher than SGR (0.11±0.008 d-1) in fed-batch culture mode. In the second experiment, there was a statistically significant difference in cell density between the treatments (P=0.039) whereas in additional CO2 -treated culture had higher cell density. There was no significant difference in SGR between the treatments (RAS air = 0.27±0.017 d-1, room air = 0.25±0.006 d-1). On the other hand nitrate consumption rate was significantly higher in the RAS air treatment, i.e. with the additional CO2. The concentration of astaxanthin did not differ significantly between the treatments. Additional CO2 retained the medium's pH at a lower level and likely had a positive influence on algal growth and nutrient absorption. These results imply that the growth of H. pluvialis increased with the addition of CO2 from RAS in batch culture mode.
first_indexed 2023-07-03T20:12:34Z
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Improvements in sustainability, resource efficiency, and the \ngeneration of a valuable carotenoid pigment (astaxanthin) may all result from \nconverting wastewater nutrients into H. pluvialis biomass in RAS. In this master's \nthesis, I have done two experiments. In the first one, I examined the suitability of \nthe culture mode by comparing the growth of H. pluvialis for 17 days in RAS WW \nunder batch (input all the culture medium once) and fed-batch (input culture \nmedium gradually) culture modes. In the second experiment of 15 days, I \nexamined the algal growth and pH of the medium for 8 days until the microalga \nreach the stationary phase, nitrate removal (15 days), and astaxanthin \nconcentration (on day 15) in Modified Wright's Cryptophyte (MWC) medium \nwith batch culture mode by supplying air either from the RAS trickling filter \nwhere CO2 concentration was 964\u00b1 16.38 ppm or room air where CO2 was 451\u00b1\n1.77 ppm. In the 1st experiment, the specific growth rate (SGR) (0.20\u00b10.014 d-1) of \nthe microalga culturing in batch culture mode was significantly higher than SGR \n(0.11\u00b10.008 d-1) in fed-batch culture mode. In the second experiment, there was a \nstatistically significant difference in cell density between the treatments (P=0.039)\nwhereas in additional CO2 -treated culture had higher cell density. There was no \nsignificant difference in SGR between the treatments (RAS air = 0.27\u00b10.017 d-1, \nroom air = 0.25\u00b10.006 d-1). On the other hand nitrate consumption rate was \nsignificantly higher in the RAS air treatment, i.e. with the additional CO2. The \nconcentration of astaxanthin did not differ significantly between the treatments. \nAdditional CO2 retained the medium's pH at a lower level and likely had a \npositive influence on algal growth and nutrient absorption. 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spellingShingle Rakib, Mazharul Effects of culture mode and CO2 supply on the efficiency of Haematococcus pluvialis cultivation in recirculating aquaculture system’s wastewater Akvaattiset tieteet Aquatic sciences 4012 mikrolevät hiilidioksidi kasvu suodatus microalgae carbon dioxide growth filtration
title Effects of culture mode and CO2 supply on the efficiency of Haematococcus pluvialis cultivation in recirculating aquaculture system’s wastewater
title_full Effects of culture mode and CO2 supply on the efficiency of Haematococcus pluvialis cultivation in recirculating aquaculture system’s wastewater
title_fullStr Effects of culture mode and CO2 supply on the efficiency of Haematococcus pluvialis cultivation in recirculating aquaculture system’s wastewater Effects of culture mode and CO2 supply on the efficiency of Haematococcus pluvialis cultivation in recirculating aquaculture system’s wastewater
title_full_unstemmed Effects of culture mode and CO2 supply on the efficiency of Haematococcus pluvialis cultivation in recirculating aquaculture system’s wastewater Effects of culture mode and CO2 supply on the efficiency of Haematococcus pluvialis cultivation in recirculating aquaculture system’s wastewater
title_short Effects of culture mode and CO2 supply on the efficiency of Haematococcus pluvialis cultivation in recirculating aquaculture system’s wastewater
title_sort effects of culture mode and co2 supply on the efficiency of haematococcus pluvialis cultivation in recirculating aquaculture system s wastewater
title_txtP Effects of culture mode and CO2 supply on the efficiency of Haematococcus pluvialis cultivation in recirculating aquaculture system’s wastewater
topic Akvaattiset tieteet Aquatic sciences 4012 mikrolevät hiilidioksidi kasvu suodatus microalgae carbon dioxide growth filtration
topic_facet 4012 Akvaattiset tieteet Aquatic sciences carbon dioxide filtration growth hiilidioksidi kasvu microalgae mikrolevät suodatus
url https://jyx.jyu.fi/handle/123456789/88175 http://www.urn.fi/URN:NBN:fi:jyu-202307034318
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