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[{"key": "dc.contributor.advisor", "value": "Jernfors, Toni", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Watts, Phillip", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Koskela, Esa", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Karvinen, Sira", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Rajala, Milla", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2023-06-28T07:44:54Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2023-06-28T07:44:54Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2023", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/88094", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Geenikopioiden m\u00e4\u00e4r\u00e4n vaihtelu on yksi geneettisen eroavuuden muodoista, joskin se on paljon v\u00e4hemm\u00e4n tutkittu yhden nukleotidin muutoksiin verrattuna. Viime vuosina geenikopiom\u00e4\u00e4rien vaihtelu on yhdistetty useisiin monitekij\u00e4isiin sairauksiin sek\u00e4 muun muassa metabolian s\u00e4\u00e4telyyn, ja sen on ehdotettu olevan geneettinen mekanismi eri ymp\u00e4rist\u00f6ihin sek\u00e4 stressitekij\u00f6ihin sopeutumisessa. Geenikopioiden m\u00e4\u00e4r\u00e4n vaihtelun mahdollisesta yhteydest\u00e4 aerobiseen suorituskykyyn on kuitenkin hyvin v\u00e4h\u00e4n tutkimuksia. K\u00e4ytin korkean (HCR)- ja matalan (LCR) synnynn\u00e4isen suorituskyvyn omaavia rottalinjoja selvitt\u00e4\u00e4kseni, miten synnynn\u00e4inen aerobinen suorituskyky, vanheneminen ja juokseminen vaikuttavat ribosomaalisen DNA:n (rDNA) ja mitokondriaalisen DNA:n (mtDNA) kopiom\u00e4\u00e4riin. Lis\u00e4ksi selvitin, korreloiko mtDNA:n kopiom\u00e4\u00e4r\u00e4 mitokondriaalisten markkereiden kanssa. rDNA:n 18S ja mtDNA:n 12S alayksik\u00f6iden kopiom\u00e4\u00e4r\u00e4t m\u00e4\u00e4ritettiin kvantitatiivisella PCR:ll\u00e4 yhteens\u00e4 60:st\u00e4 lihas- ja maksakudosn\u00e4ytteest\u00e4. rDNA:n kopiom\u00e4\u00e4r\u00e4 oli korkeampi HCR rotilla molemmissa kudoksissa. Lihaksessa rDNA:n kopiom\u00e4\u00e4r\u00e4 oli nuorilla HCR rotilla selke\u00e4sti korkeampi kuin nuorilla LCR-rotilla; vanhoilla rotilla merkitsev\u00e4\u00e4 eroa rDNA:n kopiom\u00e4\u00e4riss\u00e4 HCR ja LCR rottien v\u00e4lill\u00e4 ei havaittu. Juokseminen taas lis\u00e4si rDNA:n kopiom\u00e4\u00e4r\u00e4n hajontaa, mutta ei tilastollisesti merkitsev\u00e4sti. mtDNA:ssa ei havaittu merkitsevi\u00e4 muutoksia kopiom\u00e4\u00e4r\u00e4ss\u00e4 HCR ja LCR rottien v\u00e4lill\u00e4; juokseminen kuitenkin johti suurempaan mtDNA:n kopiom\u00e4\u00e4r\u00e4n hajontaan LCR-rotilla. Lis\u00e4ksi mtDNA korreloi merkitsev\u00e4sti mitokondriaalisista markkereista vain PGC1-\u03b1:n kanssa. Tulevaisuudessa tutkimus tulisi toistaa suuremmalla n\u00e4ytem\u00e4\u00e4r\u00e4ll\u00e4 sek\u00e4 k\u00e4ytt\u00e4m\u00e4ll\u00e4 useampia kudoksia ja eli\u00f6ryhmi\u00e4. qPCR:ll\u00e4 saatuja tuloksia voisi my\u00f6s verrata ddPCR:ll\u00e4, DNA hybridisaatiolla (non-radioactive quantitative hybridization (NQG)), tai uuden sukupolven sekvensoinnilla saataviin tuloksiin. Tutkimukseni mukaan rDNA:n kopiom\u00e4\u00e4r\u00e4 saattaa periyty\u00e4 yhdess\u00e4 synnynn\u00e4isen aerobisen suorituskyvyn kanssa. Stressitekij\u00e4t taas saattavat lis\u00e4t\u00e4 rDNA:n variaation m\u00e4\u00e4r\u00e4\u00e4, mutta tulokset voivat kuitenkin olla riippuvia kudostyypist\u00e4.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Gene copy number variation (CNV) has been proposed to provide a genetic mechanism for organisms to adapt to different environments and stressors. CNV is associated with diverse types of phenotypic variation, including expression of diverse complex diseases and regulation of metabolism; however, the possible role of CNVs in an individual\u2019s intrinsic aerobic capacity is unknown. To study the CNV of ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA) in relation to intrinsic aerobic capacity, I utilized rat lines that had been artificially selected for high-capacity running (HCR) and low-capacity running (LCR). Additionally, the effects of aging and exercise on the rDNA and mtDNA copy number (CN) were quantified. The CN of 18S rDNA and 12S mtDNA loci were determined from both liver and skeletal muscle (gastrocnemius) tissues of 60 HCR and LCR rats using quantitative PCR (qPCR). HCR rats had significantly more copies of rDNA (in both tissues) than LCR rats. In skeletal muscle, young HCR rats had significantly more copies of rDNA when compared to the young LCR rats, but no difference was observed between the aged rats. Exercise, on the other hand, seemed to result in a higher variance of rDNA CN in muscle; however, the results were not significant. I found mtDNA CN to be more variable than rDNA CN, wherein LCR rats, running resulted in increased variance in muscle. mtDNA CN positively correlated with proliferator-activated receptor gamma coactivator 1-alpha (PGC1-\u03b1) but not with any of the other measured mitochondrial markers. In the future, the generality of these qPCR results should be examined in additional tissues, with more samples, and in other taxa. Additionally, it would be informative to compare the qPCR protocol with other methods of measuring locus copy number, such as droplet digital PCR (ddPCR), non-radioactive quantitative hybridization (NQH), or next-generation sequencing. This study indicates that rDNA CN can respond to long-term artificial selection, with a higher copy number being associated with higher intrinsic aerobic capacity. Additionally, environmental stressors might change the variance of rDNA CN, but the effects may be tissue specific.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2023-06-28T07:44:54Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2023-06-28T07:44:54Z (GMT). No. of bitstreams: 0\n Previous issue date: 2023", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "50", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "aerobic fitness", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "copy number variation", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "sport genetics", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Effects of intrinsic aerobic capacity, exercise, and aging on ribosomal and mitochondrial DNA copy numbers in rat skeletal muscle and liver", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202306284185", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": null, "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "epigenetiikka", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "DNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "epigenetics", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "DNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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