Dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability

Evolution of dietary niche and gut microbiota may be driven by natural selection acting on behavioral or physiological traits. However, relative importance of genetic and environmental components remain unclear. In this thesis I conducted a lab-to-field translocation experiment using bank vole se...

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Main Author: Vuori, Tommi
Other Authors: Matemaattis-luonnontieteellinen tiedekunta, Faculty of Sciences, Bio- ja ympäristötieteiden laitos, Department of Biological and Environmental Science, Jyväskylän yliopisto, University of Jyväskylä
Format: Master's thesis
Language:eng
Published: 2023
Subjects:
Online Access: https://jyx.jyu.fi/handle/123456789/88083
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author Vuori, Tommi
author2 Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_facet Vuori, Tommi Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä Vuori, Tommi Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_sort Vuori, Tommi
datasource_str_mv jyx
description Evolution of dietary niche and gut microbiota may be driven by natural selection acting on behavioral or physiological traits. However, relative importance of genetic and environmental components remain unclear. In this thesis I conducted a lab-to-field translocation experiment using bank vole selection lines with artificially enhanced capability to maintain body mass with low-quality fiber-rich diet (H-line) and unselected control lines (C-line). Objectives of this study were to investigate 1) if overall genotypic differences that result from artificial selection for whole-organism performance are coupled with dietary niche divergence and 2) whether responses of gut microbiota of the selection lines differ when translocated from field to laboratory. Realized diets of the bank voles in the field conditions were determined using stable isotopes analysis for carbon and nitrogen in bank vole liver tissues. Gut microbial responses were examined using repeated fecal sampling and 16S rRNA-V4 amplicon sequencing. Indicated by stable isotopes of nitrogen, H-line appeared to consume food from the lower trophic level than C-line. Moreover, interestingly, carbon sources differed between the selection lines in juveniles but not in adults. However, environmental variation seemed to explain remarkable proportion of dietary variation. While translocation from the laboratory to field conditions induced different gut microbial responses between lines, the effects of environmental factors in relation to host traits were notable. Taken together, the results presented in this thesis are in line with previous research and indicate that the selection for physiological or behavioral performance can result to modifications in dietary niche and gut microbiota. These results call for further research on the assessment of long-term trends of these findings.
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However, relative importance of \ngenetic and environmental components remain unclear. In this thesis I conducted \na lab-to-field translocation experiment using bank vole selection lines with \nartificially enhanced capability to maintain body mass with low-quality fiber-rich \ndiet (H-line) and unselected control lines (C-line). Objectives of this study were \nto investigate 1) if overall genotypic differences that result from artificial \nselection for whole-organism performance are coupled with dietary niche \ndivergence and 2) whether responses of gut microbiota of the selection lines \ndiffer when translocated from field to laboratory. Realized diets of the bank voles\nin the field conditions were determined using stable isotopes analysis for carbon \nand nitrogen in bank vole liver tissues. Gut microbial responses were examined \nusing repeated fecal sampling and 16S rRNA-V4 amplicon sequencing. Indicated \nby stable isotopes of nitrogen, H-line appeared to consume food from the lower \ntrophic level than C-line. Moreover, interestingly, carbon sources differed \nbetween the selection lines in juveniles but not in adults. However, \nenvironmental variation seemed to explain remarkable proportion of dietary \nvariation. While translocation from the laboratory to field conditions induced \ndifferent gut microbial responses between lines, the effects of environmental \nfactors in relation to host traits were notable. Taken together, the results \npresented in this thesis are in line with previous research and indicate that the \nselection for physiological or behavioral performance can result to modifications \nin dietary niche and gut microbiota. 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spellingShingle Vuori, Tommi Dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability experimental evolution trophic niche stable isotopes 16S rRNA Ekologia ja evoluutiobiologia Ecology and evolutionary biology 4011 metsämyyrä myyrät isotoopit suolistomikrobisto mikrobisto Clethrionomys glareolus voles isotopes gastrointestinal microbiota microbiome
title Dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability
title_full Dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability
title_fullStr Dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability Dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability
title_full_unstemmed Dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability Dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability
title_short Dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability
title_sort dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability
title_txtP Dietary niche and gut microbial responses to novel environment in bank voles artificially selected for herbivorous capability
topic experimental evolution trophic niche stable isotopes 16S rRNA Ekologia ja evoluutiobiologia Ecology and evolutionary biology 4011 metsämyyrä myyrät isotoopit suolistomikrobisto mikrobisto Clethrionomys glareolus voles isotopes gastrointestinal microbiota microbiome
topic_facet 16S rRNA 4011 Clethrionomys glareolus Ecology and evolutionary biology Ekologia ja evoluutiobiologia experimental evolution gastrointestinal microbiota isotoopit isotopes metsämyyrä microbiome mikrobisto myyrät stable isotopes suolistomikrobisto trophic niche voles
url https://jyx.jyu.fi/handle/123456789/88083 http://www.urn.fi/URN:NBN:fi:jyu-202306274145
work_keys_str_mv AT vuoritommi dietarynicheandgutmicrobialresponsestonovelenvironmentinbankvolesartificiallyselected