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[{"key": "dc.contributor.author", "value": "Valkonen, Sofia", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2023-05-25T05:50:42Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2023-05-25T05:50:42Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2022", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/87167", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Antibiootit ovat yksi modernin historian menestyksekk\u00e4immist\u00e4 l\u00e4\u00e4ketieteellisist\u00e4 l\u00f6yd\u00f6ist\u00e4, ja niill\u00e4 on ollut t\u00e4rke\u00e4 rooli patogeenisten bakteerien aiheuttamien infektioiden torjunnassa. Vuosikymmeni\u00e4 kest\u00e4nyt antibioottien laaja ja ep\u00e4spesifinen k\u00e4ytt\u00f6 on kuitenkin johtanut antibioottiresistenssin ja monille antibiooteille resistenttien patogeenien yleistymiseen maailmanlaajuisesti. Yhten\u00e4 esimerkkin\u00e4 antibioottiresistenttien patogeenien aiheuttamasta infektiosta on Escherichia coli -bakteerin aiheuttamat virtsatietulehdukset, jotka ovat yksi yleisimmist\u00e4 tartuntataudeista maailmanlaajuisesti. Tutkimuksen tavoitteena oli l\u00f6yt\u00e4\u00e4 toiminnallisia hoitoyhdistelmi\u00e4 kolmen eri E. coli -kannan (EC7, EC10 ja EC38) hoitoon yhdistelem\u00e4ll\u00e4 kolmea eri antibiootia (trimetopriimi, sulfametoksatsoli ja nitrofurantoiini) ja viitt\u00e4 eri bakteeriofaagia (5 eri faagia/bakteeri). Optisen tiheyden (OD600) mittauksia k\u00e4ytettiin n\u00e4ytteen solutiheytt\u00e4 tarkastellessa. Hypoteesina oli, ett\u00e4 kontrollien ja eri hoitomenetelmien absorbanssiarvojen v\u00e4lill\u00e4 olisi merkitsev\u00e4 ero 26 tunnin mittausten j\u00e4lkeen. Antibiootteja ja faageja yhdistelem\u00e4ll\u00e4 voitiin tehokkaasti eliminoida E. coli bakteerit biofilmeist\u00e4. Yhdistelm\u00e4menetelm\u00e4t, joissa yhdistettiin faagicocktaileja ja antibiootticocktaileja olivat tehokkaita. Tehokkain l\u00e4hestymistapa kuitenkin vaihteli bakteerien v\u00e4lill\u00e4, ja sen vuoksi hoidon tehokkuus pit\u00e4\u00e4 arvioida erikseen kussakin faagi-bakteeri j\u00e4rjestelm\u00e4ss\u00e4. Lis\u00e4ksi j\u00e4rjestelm\u00e4n mahdollisesti antagonistinen vaikutus vaatii tunnistamista ja voi vaikuttaa suuresti hoidon tehokkuuteen. Koska antibioottiresistenssin levi\u00e4minen on vakava huolenaihe maailmanlaajuisesti, toiminnallisten yhdistelm\u00e4hoito sovellutuksien l\u00f6yt\u00e4minen on t\u00e4rke\u00e4\u00e4 ja niiden avulla voidaan jatkossakin hoitaa E. coli -bakteerin aiheuttamia virtsatieinfektioita ja s\u00e4ilytt\u00e4\u00e4 hoitopotentiaali my\u00f6s tulevaisuudessa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Antibiotics are one of the most successful medical discoveries of modern history and have played a vital role in combating infections caused by pathogenic bacteria. However, decades of extensive and non-specific use have led to the widespread emergence of antibiotic-resistance and multidrug-resistant pathogens, creating a threat known as the post-antibiotic era. Escherichia coli-caused urinary tract infections are among the most common infectious diseases globally. Thus, there is an increasing need for new treatment approaches against these pathogens. The focus of the study was to find functional therapy combinations for treating three different E. coli strains (EC7, EC10 and EC38) combined with three different antibiotics (trimethoprim, sulfamethoxazole, and nitrofurantoin) and bacteriophages (5 different strains/host). Optical density (OD600) measurements were used to observe the cellular density of each strain previously exposed to different treatment approaches. The hypothesis was that the absorbance max of the various treatment approaches after 26h would not be the same across the treatment approaches. The combinational treatment possessed the potential to eliminate E. coli in bacterial biofilms and resulted in high treatment efficacy. However, the best approach varied between the hosts and therefore required evaluating the most efficient treatment within each phage-host system. Additionally, the possibly antagonistic effect of the system requires identification and can significantly affect the treatment efficacy. The widespread emergence of antibiotic resistance is a severe concern worldwide; fortunately, the treatment functionality can be maintained with combinational therapy applications that can eradicate bacterial biofilms and subsequently treat antibiotic-resistant pathogens causing urinary tract infections.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2023-05-25T05:50:42Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2023-05-25T05:50:42Z (GMT). No. of bitstreams: 0\n Previous issue date: 2022", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "47", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.title", "value": "Effects of Combined Use of Bacteriophages and Antibiotics to Eliminate Escherichia coli in Bacterial Biofilm", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202305253231", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibioottiresistenssi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteriofagit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fagiterapia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiootit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "biofilmit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "virtsatieinfektio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiotic resistance", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bacteriophages", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "phage therapy", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiotics", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "biofilms", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "urinary tract infection", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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