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[{"key": "dc.contributor.advisor", "value": "Kalenius, Elina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Asikainen, Harri", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2023-05-08T06:23:38Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2023-05-08T06:23:38Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2023", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/86793", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "T\u00e4m\u00e4n ty\u00f6n tarkoituksena oli kehitt\u00e4\u00e4 ja validoida menetelm\u00e4 Jyv\u00e4skyl\u00e4n yliopiston Kemian \nlaitoksella jo valmiiksi olevalle HPLC-ESI-QqQ-laitteistolle siten, ett\u00e4 menetelm\u00e4ll\u00e4 saataisiin \nkvantitatiivisesti m\u00e4\u00e4ritetty\u00e4 kortikosteroni- ja testosteronipitoisuudet hyvin v\u00e4h\u00e4isest\u00e4 m\u00e4\u00e4r\u00e4st\u00e4 linnun veriplasmaa ilman n\u00e4ytteiden derivatisointia. Laiteparametrien optimointi suoritettiin kokeilemalla eri parametrien vaikutusta testosteronin ja kortikosteronin erottuvuuteen ja \nvasteeseen ja valitsemalla parametreista sellaiset, joilla kyseisten yhdisteiden erottuvuus oli selkein ja vaste suurin. Ty\u00f6ss\u00e4 optimoidulla menetelm\u00e4ll\u00e4 saadaan m\u00e4\u00e4ritetty\u00e4 kvantitatiivisesti \nkortikosteroni ja testosteroni 10 mikrolitrasta linnun veriplasmaa ilman n\u00e4ytteiden derivatisointia suuruusluokassa ng/ml. Ty\u00f6ss\u00e4 saadut m\u00e4\u00e4ritysrajat (LOQ) olivat kortikosteronille \n2,72 ng/ml ja testosteronille 3,17 ng/ml. Saadut m\u00e4\u00e4ritysrajat ovat selv\u00e4sti liian suuret, sill\u00e4 jos \nm\u00e4\u00e4ritysrajoina k\u00e4ytett\u00e4isiin pienimm\u00e4n pitoisuuden standardiliuoksia, niin kortikosteronille \nm\u00e4\u00e4ritysrajaksi tulisi 1,18 ng/ml (S/N = 12,5) ja testosteronille 0,98 ng/ml (S/N = 39,1). Korkean signaali-kohinasuhteen takia testosteronille saataisiin viel\u00e4 t\u00e4t\u00e4kin huomattavasti alhaisempi m\u00e4\u00e4ritysraja. Hajonnat kaikissa ty\u00f6ss\u00e4 tehdyiss\u00e4 mittauksissa olivat alle 15 % (CV%) ja \nQC-n\u00e4ytteiden tarkkuudet testosteronille olivat 94\u2013101 %, mutta kortikosteronille 82\u201383 %. \nKortikosteronin alhaisiin tarkkuuksiin saattoi vaikuttaa k\u00e4ytetty sis\u00e4inen standardi (13C3-testosteroni), joka korjasi kortikosteronin kalibrointisuoran kulmakertoimen liian jyrk\u00e4ksi. Kalibrointisuorien selitysasteet olivat kortikosteronille 0,9939 ja testosteronille 0,9954.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2023-05-08T06:23:38Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2023-05-08T06:23:38Z (GMT). No. of bitstreams: 0\n Previous issue date: 2023", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "39", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.title", "value": "HPLC-MS/MS-menetelm\u00e4n kehitt\u00e4minen testosteronin ja kortikosteronin kvantitatiiviseksi m\u00e4\u00e4ritt\u00e4miseksi linnun veriplasmasta", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202305082883", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Kemian laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Chemistry", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Analyyttinen kemia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Analytical Chemistry", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": null, "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "40311", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "testosteroni", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kortikosteroni", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suuren erotuskyvyn nestekromatografia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "massaspektrometria", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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