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[{"key": "dc.contributor.advisor", "value": "Haapala, Eero", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Aatsinki, Anna", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Pahkala, Katja", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Boelius, Hanna-Mari", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2023-05-03T04:41:12Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2023-05-03T04:41:12Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2023", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/86727", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Kasvava tieteellinen n\u00e4ytt\u00f6 yhdist\u00e4\u00e4 fyysisen aktiivisuuden sek\u00e4 suolistomikrobit. Tutkimusten perusteella fyysisell\u00e4 aktiivisuudella n\u00e4ytt\u00e4isi olevan hy\u00f6dyllisi\u00e4 vaikutuksia suolistomikrobistoon ja toisaalta suolistomikrobistolla vaikutusta esimerkiksi fyysiseen suorituskykyyn. Suurin osa tutkimuksesta on painottunut i\u00e4kk\u00e4isiin, henkil\u00f6ihin, joilla on ylipainoa ja urheilijoihin sek\u00e4 korkeaan liikuntam\u00e4\u00e4r\u00e4\u00e4n. Laajalla v\u00e4est\u00f6ll\u00e4 tehdyt tutkimukset ovat olleet v\u00e4h\u00e4isi\u00e4. \nT\u00e4m\u00e4n poikkileikkaustutkimuksen tavoitteena oli tutkia vapaa-ajan liikunnan ja suolistomikrobiston v\u00e4list\u00e4 yhteytt\u00e4. Suolistomikrobistodata ulosten\u00e4ytteist\u00e4 ja vapaa-ajan liikunta-aktiivisuus ker\u00e4ttiin 302 henkil\u00f6lt\u00e4, jotka osallistuivat Sepelvaltimotaudin riskitekij\u00f6iden interventioprojektin (STRIP) seurantaan 26-vuotiaina. Tiedot vapaa-ajan liikunnasta ker\u00e4ttiin itset\u00e4ytett\u00e4v\u00e4ll\u00e4 kyselyll\u00e4. Suolistomikrobin\u00e4ytteet profiloitiin k\u00e4ytt\u00e4m\u00e4ll\u00e4 16S rRNA sekvenssiteknologiaa. Kaikki tutkimuksen analyysit suoritettiin R ohjelmassa soveltuvilla mikrobianalyysipaketteja. Vapaa-ajan liikunta-aktiivisuuden perusteella henkil\u00f6t jaettiin seuraaviin ryhmiin: matalan liikunta-aktiivisuuden ryhm\u00e4 (n = 83, \u22645MET/h/vko) ja korkean liikunta-aktiivisuuden ryhm\u00e4 (n = 219,\u226516MET/h/vko)\nAlfadiversiteetti\u00e4 tutkittiin Shannonin indeksill\u00e4 sek\u00e4 Wilcoxonin testill\u00e4. Betadiversiteetin analyysi tehtiin permutaatio varianssianalyysill\u00e4 (PERMANOVA), k\u00e4ytt\u00e4en adonis2 funktiota vegan-paketissa, sek\u00e4 Bray-Curtisin ja Jaccardin dissimilariteetti-indeksej\u00e4. Differentiaalista esiintymistiheytt\u00e4 tutkittiin k\u00e4ytt\u00e4m\u00e4ll\u00e4 DESeq2 ja ALDEx2 menetelmi\u00e4. Alfa- ja betadiversiteetti analyysit suoritettiin koko datalle. DESeq2 ja ALDEx2 analyysit tehtiin v\u00e4h\u00e4isen pitoisuuden lajit poistettuina analyysista. Tulosten visualisointi tehtiin R-ohjelmassa moniulotteisella skaalauksella. Raportoinnissa hy\u00f6dynnettiin soveltuvin osin STORM-ohjeistusta.\nHavaitsimme, ett\u00e4 vaikka alfadiversiteetti oli ryhmien v\u00e4lill\u00e4 samanlainen, betadiversiteetti erosi merkitsev\u00e4sti. Eroa betadiversiteetiss\u00e4 ajoivat Prevotella ja Paraprevotella korkean, sek\u00e4 Bacteroides matalan liikunta-aktiivisuuden ryhm\u00e4ss\u00e4. Esiintymistiheysanalyysissa DESeq2 tunnisti 18 ja ALDEx2 kolme sukua, jotka erosivat merkitsev\u00e4sti ryhmien v\u00e4lill\u00e4. \nKohtalainen liikunta-annos on yhteydess\u00e4 betadiversiteettiin ja esiintymistiheyden lis\u00e4\u00e4ntymiseen tietyiss\u00e4 suvuissa, kuten Prevotella-, Veillonella- ja Lactobacillus, jotka on aikaisemmin yhdistetty kuormittavampaan liikuntaan.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "There is increasing evidence of interaction between physical activity and gut microbiota. Research data suggest of beneficial effects of physical activity to gut microbiota and potential effect of gut microbiota to e.g., physical performance. However, the majority of the current research has focused on elderly, people who are overweight and athletes, whereas general population research is scarce.\nThe aim of this cross-sectional study was to explore the association between leisure time physical activity (LTPA) and gut microbiota (GM) composition. \u202f Gut microbiota from stool samples and LTPA were assessed in 302 adults aged 26 years who participated in the longitudinal Special Turku Coronary Risk Factor Intervention Project (STRIP). LTPA was assessed with a self-administrated questionnaire. Profiling of gut microbiota was done using 16S rRNA amplicon sequencing.\u202f All statistical analyses were performed with R programming language using suitable microbiome analysis packages. Based on their LTPA participants were divided in low (n = 83, \u22645MET/h/wk) and high (n = 219, \u226516MET/h/wk) PA groups.\nAlpha diversity was assessed by counting abundances using Shannon Index in microbiome and further Wilcoxon test. Beta diversity was analysed with Permutational Analysis of Variation (PERMANOVA) using adonis2 function in vegan and Bray-Curtis and Jaccard dissimilarity indexes. Beta dispersion analysis was done using PERMDISP2 in vegan package. For differential abundance analyses DESeq2 and ALDEx2 were used. The alpha and beta diversity analyses were performed to all data, including rare taxa. DeSeq2 and ALDEx2 were performed with rare taxa removed. Data was visualised in R with Multidimensional Scaling. Reporting occurred according to STORM guidelines as applicable.\nWe found that even though alpha diversity was similar between the high and low activity groups, beta diversity differs significantly between high and low activity groups. The difference was driven with Prevotella and Paraprevotella species in the high and genus Bacteroides in the low PA group. In differential abundance analysis DESeq2 identified 18 ja ALDEx2 three generas that were significantly different between the PA groups. A moderate dosage of LTPA is associated with beta diversity and increased abundance of certain genera, such as Prevotella-, Veillonella- and Lactobacillus, that are previously associated with more vigorous PA.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2023-05-03T04:41:12Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2023-05-03T04:41:12Z (GMT). No. of bitstreams: 0\n Previous issue date: 2023", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "58", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": null, "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "gut microbiota", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Association of Leisure Time Physical Activity with Gut Microbiota Composition\u202fin Early Adulthood : A Cross-sectional analysis from subsample of the STRIP study 26 years follow-up", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202305032822", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Liikuntatieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sport and Health Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Liikunta- ja terveystieteet", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Sport and Health Sciences", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Liikuntal\u00e4\u00e4ketiede", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Sport Medicine", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.copyright", "value": "\u00a9 The Author(s)", "language": null, "element": "rights", "qualifier": "copyright", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "5042", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "liikunta", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fyysinen aktiivisuus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suolistomikrobisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suolisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "physical training", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "physical activeness", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microbiome", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "gastrointestinal microbiota", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "intestines", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microbes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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