| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Kankare, Maaria", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Hopkins, David", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Tyukmaeva, Venera", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Puolakka, P\u00e4ivikki", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2023-02-17T12:28:29Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2023-02-17T12:28:29Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2019", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/85524", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Hy\u00f6nteisten Trypanosomatidae-heimoon kuuluvat alkuel\u00e4imet ovat laaja, joskin huonosti tunnettu loisryhm\u00e4, mutta niiden tiedet\u00e4\u00e4n vaikuttavan hy\u00f6nteisis\u00e4ntiens\u00e4 kelpoisuuteen, ravinnonhankintaan ja immuunipuolustukseen. Sen sijaan n\u00e4iden loisten vaikutusta is\u00e4nn\u00e4n kykyyn sopeutua elinymp\u00e4rist\u00f6ns\u00e4 stressitekij\u00f6ihin on tutkittu huomattavasti v\u00e4hemm\u00e4n. Hy\u00f6nteisten levinneisyyden ja selviytymisen kannalta elinymp\u00e4rist\u00f6n l\u00e4mp\u00f6tila on ehk\u00e4\nt\u00e4rkein abioottinen tekij\u00e4, ja varsinkin matalat l\u00e4mp\u00f6tilat ovat haasteellisia alueilla, joilla ilmastolliset vaihtelut ovat suuria. T\u00e4ss\u00e4 projektissa selvitettiin Trypanosomatidae-loisten vaikutuksia stressinkest\u00e4v\u00e4n Drosophila lummei - mahlak\u00e4rp\u00e4sen kylm\u00e4nkest\u00e4vyyteen, liikeaktiivisuuteen ja hengiss\u00e4 selviytymiseen. Aihetta tutkittiin geneettisill\u00e4 (kvantitatiivinen PCR) ja fenotyyppisill\u00e4 (liikeaktiivisuus- ja selviytymiskokeet) testeill\u00e4. Kylm\u00e4nkest\u00e4vyys ei korreloinut infektion m\u00e4\u00e4r\u00e4n kanssa kummallakaan sukupuolella, eik\u00e4 kylm\u00e4k\u00e4sittely\u00e4 edelt\u00e4v\u00e4ll\u00e4 ja sit\u00e4 parantavalla akklimaatiok\u00e4sittelyll\u00e4 ollut vaikutusta loisinfektion m\u00e4\u00e4r\u00e4\u00e4n. Infektion m\u00e4\u00e4r\u00e4 sen sijaan oli matalampi naarailla kuin koirailla. Liikeaktivisuus oli suurempaa ei-infektoituneilla\nk\u00e4rp\u00e4sill\u00e4 verrattuna infektoituneisiin, mutta tutkimuksissa k\u00e4ytetty valojakso ei vaikuttanut k\u00e4rp\u00e4sten aktiivisuuteen. Selviytymiskokeessa infektoitujen k\u00e4rp\u00e4sten elinik\u00e4 oli lyhyempi kuin ei-infektoitujen, mutta valojakso ei vaikuttanut\nelinik\u00e4\u00e4n. Saadaksemme uutta tietoa hy\u00f6nteisten immuunipuolustuksesta, tarvitaan my\u00f6s alkuel\u00e4ininfektiotutkimuksia koska suuri osa aiemmista tutkimuksista on keskittynyt bakteereihin ja viruksiin", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Trypanosomatids are an extremely diverse group of single cell eukaryotic parasites infecting humans, animals and plants. Insect trypanosomatids are a broad but a less well-known group of monoxeus parasites, that affect a range of their hosts\u2019 fitness traits, including feeding behavior and immunity. Also less is known about the effects of these infections on host's ability to respond to abiotic\nfactors. Temperature is perhaps the most important environmental factor governing insect distributions, fitness and survival and particularly low temperatures appear challenging for insect physiology in habitats with drastic seasonal change. The aim of this study was to investigate the effect of trypanosomatid infection on cold tolerance, survival and locomotor activity of the flies from highly stress tolerant Drosophila virilis group species, Drosophila lummei. Genetic (quantitative PCR) and phenotypic methods (locomotor activity and survival experiments) were used to study these questions. Cold tolerance was not affected by infection load in neither sex nor different acclimation treatment\ngroups, while females had lower infection load compared to males. Locomotor activity results indicated that there was a difference in total activity between infected and non-infected flies, but light cycle did not influence total activity. Moreover, infected flies had shorter lifespan than non-infected flies, but again\nlight cycle did not affect the survival. Studying gut infecting trypanosomatids in Drosophila is important as most Drosophila infection studies are only performed with bacteria and virus. Also, as trypanosomatids are known to be common parasites in insects, studies of their effects on their hosts could reveal new interesting aspects of insect immune system.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2023-02-17T12:28:28Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2023-02-17T12:28:29Z (GMT). No. of bitstreams: 0\n Previous issue date: 2019", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "51", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "liikeaktiivisuus", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "loisinfektiot", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "kvantitatiivinen PCR\n(qPCR)", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Effects of trypanosomatid infection on Drosophila lummei fruit fly", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202302171786", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ekologia ja evoluutiobiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Ecology and evolutionary biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4011", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hy\u00f6nteiset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "loiset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infektiot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kylm\u00e4nkest\u00e4vyys", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "insects", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "parasites", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infections", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cold resistance", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|