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[{"key": "dc.contributor.advisor", "value": "Yl\u00e4nne, Jari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Roslund, Marja", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Laitinen, Olli", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "J\u00e4msen, Ronja", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-12-23T07:38:02Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-12-23T07:38:02Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2022", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/84576", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Allergiat ja immuuniv\u00e4litteiset sairaudet ovat nopeasti kasvava ongelma teollistuneissa maissa. Viime vuosikymmenten aikana on k\u00e4ynyt selv\u00e4ksi, ett\u00e4 ihmisten et\u00e4\u00e4ntyminen luonnosta on yhteydess\u00e4 immuuniv\u00e4litteisten sairauksien yleistymiseen. T\u00e4m\u00e4 ImmunoGarden -tutkimus t\u00e4ht\u00e4\u00e4 immuuniv\u00e4litteisten sairauksien luonnonmukaisten ennaltaehk\u00e4isykeinojen ja hoitomuotojen kehitt\u00e4miseen, ja t\u00e4m\u00e4n pro gradu -tutkimuksen tarkoituksena on selvitt\u00e4\u00e4, muuttuvatko ihmisen suolistomikrobiston lajirunsaus ja monimuotoisuus kaupunkiymp\u00e4rist\u00f6ss\u00e4 tapahtuvan sis\u00e4viljelyn seurauksena, ja ovatko suolistomikrobiston lajirunsaus ja monimuotoisuus erilaiset eri kasvualustoja k\u00e4ytt\u00e4neiden ryhmien v\u00e4lill\u00e4. Yksi kasvualustoista on varta vasten t\u00e4t\u00e4 koetta varten suunniteltu, ja siihen on lis\u00e4tty monimuotoista mets\u00e4- ja kasvimikrobistoa. Vapaaehtoiset koehenkil\u00f6t harjoittivat kaupunkiviljely\u00e4 kolmen kuukauden ajan istuttamalla erilaisia viljelykasveja joko kompostimultaan tai mikrobirikastettuun kasvualustaan, ja heilt\u00e4 ker\u00e4ttiin ulosten\u00e4ytteet. Ulosten\u00e4ytteist\u00e4 eristettiin 16S rRNA-geenit, jotka monistettiin PCR:lla, ja niille tehtiin Illumina-sekvensointi. T\u00e4ss\u00e4 kokeessa kaupunkiymp\u00e4rist\u00f6ss\u00e4 tapahtunut sis\u00e4viljely ei merkitt\u00e4v\u00e4sti muuttanut ihmisen suolistomikrobiston lajirikkautta tai monimuotoisuutta, mutta ajan kuluessa suolistomikrobistot muuttuivat molemmilla tutkimusryhmill\u00e4 COVID19-pandemiasta johtuvan hygieniatason nousun seurauksena.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Allergies and immune-mediated diseases are a rapidly growing threat in industrialized countries. During the last few decades, it has become clearer that people distancing from nature might have something to do with this increase. This ImmunoGarden study aims for finding natural solutions for preventing and possibly curing immune-mediated diseases, and the goal for this pro gradu study is to answer the question whether the human intestinal microbiota richness and diversity change due to urban indoor farming or not and do the changes in intestinal microbiota vary in between different research groups using different seedbeds. One of the used seedbeds is designed especially for this experiment, and it has safe and diverse forest- and plant microbiota added in it. The stool samples were collected from volunteers who had done urban indoor farming for three months using either compost soil or microbe-enriched seedbed, and different plants for farming. The 16S rRNA gene was isolated from the stool samples, multiplied in PCR, and analyzed further with Illumina sequencing. With this experiment the urban indoor farming did not significantly change the human intestinal microbiota richness or diversity, but the intestinal microbiotas of the research subjects in both research groups changed through time due to the increased hygiene levels in the beginning of COVID19-pandemic.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2022-12-23T07:38:01Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-12-23T07:38:02Z (GMT). No. of bitstreams: 0\n Previous issue date: 2022", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "80", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "immune mediated diseases", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The effects of urban indoor farming for intestinal microbiota richness and diversity", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202212235816", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": 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"dc"}, {"key": "dc.subject.yso", "value": "mikrobit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suolistomikrobisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "biodiversiteetti", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "luonnon monimuotoisuus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immuunij\u00e4rjestelm\u00e4", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "intestines", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bacteria", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microbes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microbiome", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "gastrointestinal microbiota", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "biodiversity", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "natural diversity", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immune system", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", 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