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[{"key": "dc.contributor.advisor", "value": "Kivel\u00e4, Riikka", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Hulmi, Juha", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Koho, Saara", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-12-21T11:43:41Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-12-21T11:43:41Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2022", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/84541", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Liikunta aiheuttaa muutoksia luurankolihaksen supistuvissa, rakenteellisissa ja \naineenvaihdunnallisissa ominaisuuksissa, mik\u00e4 saa aikaan useita terveyshy\u00f6tyj\u00e4. N\u00e4m\u00e4 \nmuutokset tapahtuvat useiden tilap\u00e4isten s\u00e4\u00e4telymekanismien, kuten transkriptionaalisten\nreittien, aktivoitumisen seurauksena. N\u00e4it\u00e4 transkriptionaalisia mekanismeja ja niit\u00e4 s\u00e4\u00e4televi\u00e4 \ntekij\u00f6it\u00e4 ei kuitenkaan viel\u00e4 t\u00e4ysin tunneta. Verisuonten kasvu on todenn\u00e4k\u00f6isesti yksi t\u00e4rke\u00e4 \nt\u00e4llainen s\u00e4\u00e4telev\u00e4 tekij\u00e4 lihaksessa. Se on merkitt\u00e4v\u00e4 kest\u00e4vyysharjoitteluadaptaatio, joka \nparantaa aerobista kapasiteettia ja edist\u00e4\u00e4 syd\u00e4n- ja verisuoniterveytt\u00e4. T\u00e4m\u00e4n tutkielman\ntarkoituksena oli tutkia verisuonten kasvun merkityst\u00e4 luurankolihaksen transkriptomivasteissa \nkest\u00e4vyysharjoitteluun.\nVillityypin hiiret (WT) ja hiiret, joilta poistettiin verisuonen endoteelin kasvutekij\u00e4n reseptori \nVEGFR2 (R2Del) jaettiin harjoittelevaan ja kontrolliryhm\u00e4\u00e4n. Kest\u00e4vyysharjoittelu koostui\nprogressiivisesta 60 minuutin juoksuharjoittelusta juoksumatolla 5 kertaa viikossa 4 viikon \najan. Tibialis anterior -lihakset ker\u00e4ttiin viikko harjoittelun j\u00e4lkeen, ja koko transkriptomin\nlaajuinen geeniekspressioanalyysi tehtiin RNA-sekvensoinnilla. Muutoksia geenien \nilmenemisess\u00e4 ja geenien s\u00e4\u00e4telemiss\u00e4 signalointireiteiss\u00e4 analysoitiin RStudio -ohjelmistolla.\nLihasten kapillarisaatio lis\u00e4\u00e4ntyi merkitt\u00e4v\u00e4sti harjoittelun j\u00e4lkeen vain WT-hiirill\u00e4 (p < 0,001).\nLihassolukoko kasvoi merkitt\u00e4v\u00e4sti harjoittelun j\u00e4lkeen molemmilla hiirityypeill\u00e4. Eniten\nmuutoksia yksitt\u00e4isten geenien ilmenemisess\u00e4 tapahtui WT-hiirill\u00e4, mutta R2Del-hiirill\u00e4 oli\neniten muuttuneita geenien signalointireittej\u00e4 harjoittelun j\u00e4lkeen. Useat signalointireitit \naktivoituivat eri tavoin harjoittelun j\u00e4lkeen WT- ja R2Del-hiirill\u00e4. Esimerkiksi rasvahappojen \naineenvaihdunta v\u00e4heni WT-hiirill\u00e4 mutta lis\u00e4\u00e4ntyi R2Del-hiirill\u00e4 harjoittelun j\u00e4lkeen.\nHarjoittelu aiheutti pitkittyneit\u00e4 h\u00e4iri\u00f6it\u00e4 aerobisessa aineenvaihdunnassa R2Del-hiirill\u00e4 \nverrattuna WT-hiiriin, mik\u00e4 viittaa siihen, ett\u00e4 verisuonten kasvun est\u00e4minen vaikutti lihaksen \ntranskriptomin harjoitteluvasteeseen. T\u00e4t\u00e4 R2Del-hiirien erilaista harjoitusvastetta tarvitaan \nmahdollisesti koko harjoitusjakson ajan kompensoimaan hapenkuljetuksen puutetta, jonka \nverisuonten kasvun est\u00e4minen aiheuttaa. T\u00e4m\u00e4 tutkimus on t\u00e4rke\u00e4 askel kohti sek\u00e4\nkokonaisvaltaista ymm\u00e4rryst\u00e4 mekanismeista, jotka s\u00e4\u00e4telev\u00e4t adaptoitumista \nkest\u00e4vyysharjoitteluun ett\u00e4 parempaa harjoitusprotokollien suunnittelua, jossa yksil\u00f6lliset \nominaisuudet otetaan huomioon.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Exercise facilitates changes in skeletal muscle contractile, structural, and metabolic properties\nwhich results to multiple health benefits. These changes occur as signalling, epigenetic and \ntranscriptional pathways are activated transiently in response to exercise. These transcriptional \nmechanisms and the factors that regulate them are however not fully understood. Angiogenesis\nis likely an important factor facilitating the skeletal muscle responses since it is highly activated \nin response to exercise. It is also a significant endurance training adaptation improving aerobic \ncapacity and cardiovascular health. The aim of this thesis was to examine the role of\nangiogenesis in skeletal muscle transcriptomic responses to endurance training. \nWild-type (WT) and vascular endothelial growth factor receptor 2 deleted (R2Del) mice were \ndivided into sedentary and exercise groups. Endurance training included 60 min of treadmill \nrunning 5 x week for 4 weeks. Tibialis anterior muscles were collected 1 week after training \nand transcriptome-wide gene expression analysis was conducted with RNA sequencing.\nDifferential gene expression analysis and gene set enrichment analysis were done using data \nscience software RStudio.\nSkeletal muscle capillarization increased significantly after training in WT mice (p < 0.001) but \nnot in the R2Del mice. Muscle fibre size was significantly increased after training in both types \nof mice. The number of differentially expressed individual genes was the highest in the WT \nmice, but the greatest number of differentially regulated pathways was in the R2Del mice after \ntraining. Multiple pathways were differently activated after training in WT and R2Del mice.\nFor instance, fatty acid metabolism was downregulated in WT mice but upregulated in R2Del \nmice after training. \nAerobic exercise training caused prolonged perturbations in aerobic metabolism pathways in \nthe R2Del mice compared to the WT mice, which suggests that inhibition of angiogenesis had \na profound effect on transcriptomic exercise response in skeletal muscle. This differential \ntranscriptomic training response in R2Del mice is possibly needed throughout the training \nperiod to compensate the lack of increased capillarization and capacity to deliver oxygen. This \nstudy is an important step towards both comprehensive understanding of the mechanisms that \nregulate adaptations to endurance training and better designing of training protocols for \nindividuals with distinct physiological conditions.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2022-12-21T11:43:41Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-12-21T11:43:41Z (GMT). No. of bitstreams: 0\n Previous issue date: 2022", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "64", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "angiogenesis", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "vascular endothelial growth factor", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "endurance training", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "gene expression", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Role of angiogenesis in skeletal muscle transcriptomic responses to endurance training", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202212215787", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Liikuntatieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sport and Health Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Liikunta- ja terveystieteet", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Sport and Health Sciences", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Liikuntafysiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Exercise Physiology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "5011", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kest\u00e4vyysharjoittelu", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "verisuonet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geenit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "endurance training", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "blood vessels", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "genes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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