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[{"key": "dc.contributor.advisor", "value": "Karvonen, Anssi", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Janhunen, Matti", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Turkka, Juha-Pekka", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-10-18T05:41:26Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-10-18T05:41:26Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2022", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/83574", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Saimaan j\u00e4rvilohen (Salmo salar m. sebago) s\u00e4ilyminen Suomen el\u00e4imist\u00f6ss\u00e4 alkuper\u00e4isen\u00e4 ja geneettisesti omana kantanaan on ollut Pielisjoen Kuurnan voimalaitoksen rakentamisesta (1971) l\u00e4htien t\u00e4ysin vesiviljelyn ja istutusten varassa. Luonnonvarakeskuksen (Luke) Enonkosken kalanviljelylaitoksella s\u00e4ilytet\u00e4\u00e4n \u00e4\u00e4rimm\u00e4isen uhanalaiseksi luokitellun Saimaan j\u00e4rvilohen emokalastoja. Nyky\u00e4\u00e4n kannan s\u00e4ilymisen edellytyksen\u00e4 on luonnosta pyydettyjen emokalojen sukutuotteet, joista perustetaan kasvatukseen vuosittain aina uusi emoparvi-ik\u00e4luokka. Emokalojen kutunousu ja kalojen sukukypsyminen tapahtuu usein pitk\u00e4ll\u00e4 aikav\u00e4lill\u00e4, jonka vuoksi sukutuotteita joudutaan s\u00e4ilytt\u00e4m\u00e4\u00e4n jopa muutamia viikkoja ennen m\u00e4din hedelm\u00f6ityksi\u00e4. Pitk\u00e4t s\u00e4ilytysajat voivat heikent\u00e4\u00e4 hedelm\u00f6itystulosta ja alkioiden varhaiskehityst\u00e4, jolloin joidenkin emojen j\u00e4lkel\u00e4isi\u00e4 voidaan menett\u00e4\u00e4 kokonaan, ja siten emokalaston geneettinen monimuotoisuus pienenee. T\u00e4m\u00e4n tutkimuksen tarkoituksena oli selvitt\u00e4\u00e4 j\u00e4rvilohen sukutuotteiden s\u00e4ilytysajan vaikutusta j\u00e4lkel\u00e4isten varhaiseen elinkelpoisuuteen (poikasten normaali kehitys ja elossa s\u00e4ilyminen). M\u00e4din ovaarionesteen pH-arvoa seurattiin kahden viikon s\u00e4ilytysjakson aikana. Maidin laadun muutosta seurattiin s\u00e4ilytyksen aikana tietokoneavusteisen siitti\u00f6iden liikkuvuusanalyysin (CASA) avulla. Emokalojen paritukset suoritettiin matriisihedelm\u00f6ityksin ja jokainen yhdistelm\u00e4 (t\u00e4ysperhe) haudottiin erillisiss\u00e4 haudontayksik\u00f6iss\u00e4. N\u00e4in aineistosta voitiin arvioida my\u00f6s vanhempien suhteellisia vaikutuksia j\u00e4lkel\u00e4isten varhaista elinkyky\u00e4 kuvaaviin ominaisuuksiin. Sukutuotteiden s\u00e4ilytysajan pidentyess\u00e4 alkioiden elinkyky, ovaarionesteiden pH-arvot sek\u00e4 siitti\u00f6iden liikkuvuus ja uintinopeus laskivat merkitsev\u00e4sti. Poikasten elinkyvyss\u00e4 eri naaraiden ja naaras-koirasyhdistelmien (perheiden) v\u00e4lill\u00e4 oli ajan suhteen merkitt\u00e4v\u00e4\u00e4 vaihtelua. My\u00f6s siitti\u00f6iden liikkuvuus naaraiden ovaarionesteess\u00e4 aktivoituna vaihteli merkitsev\u00e4sti ajan suhteen eri koiraiden ja perheiden v\u00e4lill\u00e4. Tulosten perusteella j\u00e4rvilohen sukutuotteiden s\u00e4ilytt\u00e4minen v\u00e4hent\u00e4\u00e4 j\u00e4lkel\u00e4isten elinkyky\u00e4, joten sukutuotteiden s\u00e4ilytysaika on mahdollisuuksien mukaan syyt\u00e4 minimoida.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "In recent years, an increasing number of wild fish species and populations have declined due to human activities. The critically endangered Saimaa landlocked salmon (Salmo salar m. sebago) has been dependent on stockings of hatchery-reared juveniles since the building of Kuurna hydroelectric power plant in River Pielisjoki in 1971. Enonkoski aquaculture station of the Natural Resources Institute Finland (Luke) maintains the hatchery broodstocks of the Saimaa landlocked salmon, which are renewed annually using wild-caught spawners (founders) from the River Pielisjoki and periodically from the River Lieksanjoki. Short-term post-stripping storage of eggs and sperm lasting up to a few weeks is typically needed before fertilizations. Variable storage periods can decrease fertilization success and early embryonic development. It is possible that the offspring of some founders are completely lost, which decreases genetic diversity of the broodstock. The aim of this study was to investigate the effect of storage time of landlocked salmon gametes on early viability of offspring (normal development and survival of the alevins). The pH of ovarian fluid was monitored during the storage period of two weeks. The change in quality of sperm was monitored using computer-assisted sperm motility analysis (CASA). Founder matings were performed using full-factorial designs and each combination (full-sib family) was incubated in separate hatching units. This allowed evaluation of the relative influence of parents (male and female) on the early viability traits of the offspring. The viability of embryos, the pH values of ovarian fluids and the motility and swimming speed of sperm decreased significantly with increasing storage time of the gametes. There was also significant variation in the viability of the offspring between different females and families. The change in sperm motility (activated in ovarian fluids) also varied significantly between different males and families. The results suggest that the storage of landlocked salmon eggs and sperm reduces the viability of offspring, so the storage time of gametes should be minimized if possible.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2022-10-18T05:41:25Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-10-18T05:41:26Z (GMT). No. of bitstreams: 0\n Previous issue date: 2022", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "30", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "j\u00e4lkel\u00e4isten elinvoimaisuus", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "maidin s\u00e4ilytys", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "m\u00e4din s\u00e4ilytys", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "pH", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "siitti\u00f6iden liikeanalyysi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "sukutuotteiden laatu", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "s\u00e4ilytysviljely", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "uhanalaisuus", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Saimaan j\u00e4rvilohen (Salmo salar m. sebago) sukutuotteiden s\u00e4ilytysajan vaikutus j\u00e4lkel\u00e4isten varhaiseen elinkelpoisuuteen", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, 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"contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Akvaattiset tieteet", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Aquatic sciences", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", 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