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[{"key": "dc.contributor.advisor", "value": "Pasonen-Sepp\u00e4nen, Sanna", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Takabe, Piia", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Yl\u00e4nne, Jari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Korkiam\u00e4ki, Riku", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-06-23T08:08:22Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-06-23T08:08:22Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2022", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/82010", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Melanooma- ja immuunisolujen v\u00e4linen vuorovaikutus mahdollistaa melanooman kehittymisen pahanlaatuiseksi, sek\u00e4 metastaasin. Sy\u00f6p\u00e4soluista per\u00e4isin olevat solunulkoiset vesikkelit (extracellular vesicle, EV) pystyv\u00e4t eriytt\u00e4m\u00e4\u00e4n makrofaageja sy\u00f6v\u00e4lle edulliseksi fenotyypiksi, mutta tarkat mekanismit t\u00e4lle eiv\u00e4t ole viel\u00e4 tiedossa. Melanoomasoluissa hyaluronaani syntaasi -geenin ilmentymisen lis\u00e4\u00e4minen lis\u00e4\u00e4 hyaluronaanip\u00e4\u00e4llysteisten solunulkoisten vesikkeleiden erityst\u00e4. Vesikkeleit\u00e4 erittyy my\u00f6s yleisesti paljon sy\u00f6p\u00e4soluista. T\u00e4ss\u00e4 opinn\u00e4ytety\u00f6ss\u00e4 tutkin hyaluronaanip\u00e4\u00e4llysteisten solunulkoisten vesikkeleiden roolia makrofaagien erilaistumisessa. Hyaluronaanip\u00e4\u00e4llysteiset vesikkelit lis\u00e4siv\u00e4t sy\u00f6p\u00e4\u00e4 edist\u00e4vien sytokiinien ja muiden tekij\u00f6iden (IL-1\u03b2, IL-8, TSG-6, and PD-L1) ilmentymist\u00e4 makrofaageissa ja aiheuttivat makrofaageissa pitk\u00e4nomaista morfologiaa. Er\u00e4\u00e4n hyaluronaanin reseptorin, TLR4:n (Toll-like receptor 4), est\u00e4minen v\u00e4hensi sytokiinien erityst\u00e4, mik\u00e4 voi tarkoittaa hyaluronaanip\u00e4\u00e4llysteisten solunulkoisten vesikkeleiden vaikutuksen tapahtuvan TLR4:n kautta. Tutkimuksen tulokset viittaavat uuteen hyaluronaaniin liittyv\u00e4\u00e4n mekanismiin, jonka kautta makrofaagit voivat erilaistua sy\u00f6p\u00e4\u00e4 edist\u00e4v\u00e4ksi fenotyypiksi.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Malignant melanoma development and metastasis is the result of crosstalk between the tumour cells and the immune system. Tumour-derived extracellular vesicles (EVs) can differentiate macrophages into pro-tumorigenic phenotypes. However, the exact molecular mechanisms of EV-mediated macrophage differentiation are still unknown. Hyaluronan synthase (HAS) overexpression increases hyaluronan-coated EV secretion, and EV secretion is a common trait of cancer. Therefore, the differentiation of macrophages could be related to hyaluronan-coated vesicles. To test this hypothesis, macrophages were treated with melanoma-derived hyaluronan-coated vesicles and the expression of cytokines and other factors related in cancer-induced immune modulation were studied. The expression of IL-1\u03b2, IL-8, TSG-6, and PD-L1 was elevated in macrophages treated with HAS3 overexpressing melanoma cell-derived EVs (HAS3-MV3 EVs). HAS3-MV3 EVs also induced an elongated morphology in macrophages. Inhibiting TLR4 receptor decreased the cytokine expression induced by HAS3-MV3 EVs, suggesting TLR4-mediated activation. The results suggest a novel hyaluronan-related mechanism for the pro-tumorigenic differentiation of macrophages.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2022-06-23T08:08:22Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-06-23T08:08:22Z (GMT). No. of bitstreams: 0\n Previous issue date: 2022", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "64", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.title", "value": "Melanoma-derived hyaluronan-coated extracellular vesicles mediate pro-tumorigenic phenotype in macrophages", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202206233614", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "melanooma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "makrofagit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solunulkoiset vesikkelit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immuunij\u00e4rjestelm\u00e4", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immunologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hyaluronaani", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "tulehdus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "sy\u00f6p\u00e4solut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "melanoma", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "macrophages", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "extracellular vesicles", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immune system", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immunology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hyaluronan", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "inflammation", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cancer cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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