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[{"key": "dc.contributor.advisor", "value": "Takala, Heikki", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Rumfeldt, Jessica", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Ihalmo, Ville", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-06-22T11:10:56Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-06-22T11:10:56Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2022", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/81972", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Fytokromit ovat punaista ja kaukopunaista valoa aistivia reseptori proteiineja, jotka s\u00e4\u00e4telev\u00e4t geenien luentaa ja muita solutason toimintoja kasveissa ja mikrobeissa. Fytokromit koostuvat proteiinidomeeneista, jotka voidaan jakaa N-terminaalin valoa aistivaan ja C-terminaalin signaloivaan moduuliin. Fytokromien toiminta perustuu niiden ainutlaatuiseen kykyyn palautettavasti muuttua stabiilista lepotilasta valolla aktivoitavaan aktiiviseen tilaan. T\u00e4ss\u00e4 tutkielmassa keskitymme fytokromiin, joka l\u00f6ydettiin bakteerista Bradyrhizobium sp. STM 3809. T\u00e4st\u00e4 fytokromista puuttuu C-terminaalista bakteeriper\u00e4isille fytokromeille tyypillinen histidiinikinaasi ja sill\u00e4 on sen sijaan DNA:ta sitova domeeni signaloivassa moduulissa, mik\u00e4 tekee t\u00e4st\u00e4 fytokromista oivan kohteen optogenetiikan ty\u00f6kalu tutkimukseen. Tuotimme t\u00e4m\u00e4n fytokromin valoa aistivaa moduulia ja tutkimme sen ominaisuuksia ultravioletti-n\u00e4kyv\u00e4 spektroskopian ja analyyttisen geelisuodatuksen avulla. Saimme selville, ett\u00e4 t\u00e4ll\u00e4 fytokromilla on muihin tunnettuihin fytokromehin verrattuna punaiseen p\u00e4in siirtynyt absorptiomaksimi 710 nm kohdalla. Lis\u00e4ksi selvitimme, ett\u00e4 valoa aistiva moduuli, BrCBD-PHY, on monomeerinen lepotilassa ja se palautuu nopeasti takaisin lepotilaan valoaktivoinnin j\u00e4lkeen. Poikkeuksellisesti absorptiomaksimin siirtymist\u00e4 kaukopunaiseen p\u00e4in ei havaittu valaisun j\u00e4lkeen, mutta biliverdiini kromoforin todettiin silti isomerisoituvan. Huolimatta t\u00e4m\u00e4n tutkielman lupaavista l\u00f6yd\u00f6ksist\u00e4, jatkotutkimuksia tarvitaan kokopitk\u00e4ll\u00e4 fytokromilla, jotta t\u00e4m\u00e4n fytokromin potentiaalia mahdollisen optogeneettisen ty\u00f6kalun kehitt\u00e4misess\u00e4 voidaan arvioida paremmin.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Phytochromes are red and far-red light sensing signaling proteins that take part in gene expression and other cellular processes in plants and micro-organisms. Phytochromes contain two main parts, the N-terminal photosensory module and the C-terminal output module that relays light detection to other parts of the organism. Key to phytochrome function is their unique ability to reversibly switch from a stable resting state to an active state upon photoactivation. In this project, we focused on a new putative DNA-binding phytochrome discovered in the bacterium Bradyrhizobium sp. STM 3809. Unlike typical bacteriophytochromes, this phytochrome lacks a histidine kinase domain in the output module, and instead has a DNA-binding domain as the output module, which makes this phytochrome a great target for optogenetic tool research. The photosensory module of this phytochrome was expressed and characterized using ultraviolet-visible spectroscopy and analytical size-exclusion chromatography. We discovered that this phytochrome has an absorption maximum of 710 nm in the resting state, which is red shifted compared to other known phytochromes. Additionally, we learned that the photosensory module, BrCBD-PHY, is a monomer in the resting state with a fast dark reversion from the active state back to the resting state. No red shift takes place in the absorption maximum upon illumination, but the biliverdin chromophore was confirmed to undergo Z/E isomerization. Despite our promising findings in this project, further studies with the full length phytochrome are needed to assess the full potential of this phytochrome in optogenetic tool development.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2022-06-22T11:10:56Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-06-22T11:10:56Z (GMT). No. of bitstreams: 0\n Previous issue date: 2022", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "52", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "phytochrome", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Characterization of the photosensory module of a putative DNA binding phytochrome", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202206223577", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "optogenetiikka", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solubiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "optogenetics", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell biology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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