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[{"key": "dc.contributor.advisor", "value": "Ahtiainen, Maarit", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Kuopio, Teijo", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Sirki\u00e4, Onni", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-06-22T08:54:04Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-06-22T08:54:04Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2022", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/81952", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Kolorektaalisy\u00f6p\u00e4 on maailmanlaajuisesti yksi yleisimmist\u00e4 ja kuolettavimmista sy\u00f6vist\u00e4. Immuunisolujen rooli sen kehityksess\u00e4 on tunnettu jo pitk\u00e4\u00e4n. Korkea sytotoksisten T-solujen tiheys prim\u00e4\u00e4rikasvaimessa on yhdistetty parempaan kolorektaalisy\u00f6v\u00e4n ennusteeseen, mutta B-solulinjan ennustemerkitys tunnetaan huonommin. T\u00e4m\u00e4n tutkielman tarkoitus oli kartoittaa B-solujen ja plasmasolujen eri alatyyppien esiintymist\u00e4 kolorektaalisy\u00f6p\u00e4kasvaimissa sek\u00e4 arvioida niiden ennustemerkityst\u00e4. Kudosmikrosirun\u00e4ytteet 912 prim\u00e4\u00e4rikasvaimesta immuno-v\u00e4rj\u00e4ttiin B-solujen ja plasmasolujen eri alatyyppien solumerkkiaineilla multiplex-immunohistokemian avulla. QuPath-ohjelmalla tehdyn koneoppimispohjaisen kuva-analyysin tulosten perusteella n\u00e4ytteist\u00e4 havaitut B- ja plasmasolut jaettiin alatyypeikseen, ja kasvainten solutiheydet laskettiin erikseen kullekin alatyypille. Eri alatyyppien ennustemerkityksi\u00e4 tarkasteltiin sek\u00e4 Kaplan-Meierin menetelm\u00e4n ett\u00e4 Coxin regressioiden avulla. Tulosten perusteella korkea plasmasolutiheys on yhteydess\u00e4 parempaan kolorektaalisy\u00f6v\u00e4n ennusteeseen solujen alatyypist\u00e4 sek\u00e4 muista tekij\u00f6ist\u00e4 riippumatta. Havainto on ristiriidassa IgG1+ plasmasolujen hypotisoidun paremman ennustemerkityksen kanssa. B-muistisolujen ja antigeeni\u00e4 esittelevien B-solujen korkea tiheys invaasiorintaman n\u00e4ytteiss\u00e4 oli yhteydess\u00e4 parempaan ennusteeseen, kun taas kasvaimen keskiosan n\u00e4ytteiss\u00e4 merkitt\u00e4vin ennustetekij\u00e4 B-solujen osalta oli korkea naiivien B-solujen solutiheys. B-solujen ennustemerkitys oli kuitenkin riippuvainen muista tekij\u00f6ist\u00e4, mik\u00e4 viittaa niiden toimivan yhteisty\u00f6ss\u00e4 todenn\u00e4k\u00f6isesti sytotoksisten T-lymfosyyttien kanssa.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Colorectal cancer is one of the most common and deadly cancers worldwide. The significance of immune cells in colorectal tumor development is well recognized. High density of cytotoxic T cells in colorectal tumors has been associated with a better prognosis, but the prognostic importance of B lymphocyte lineage is less well understood. The aim of this study was to assess the presence of different B cell and plasma cell subsets in colorectal tumors and evaluate their prognostic importance. Tissue microarray samples from 912 primary colorectal tumors were stained with antibodies against different B cell and plasma cell markers by using multiplex immunohistochemistry. Images of the immunostained tumor tissue samples were analyzed with machine learning-based image analysis software QuPath. Detected cells were phenotyped based on the intensity of different surface markers, and cell densities were calculated for all the observed subsets. The prognostic importance of different plasma cell and B cell subsets was assessed using Kaplan-Meier estimators and Cox regression analyses. High plasma cell density had independent association with better survival regardless of the subset, thus contradicting the hypothesized superior prognostic potential of IgG1+ plasma cells. High densities of memory and antigen-presenting B cells were associated with better survival in the invasive margin, whereas high density of na\u00efve B cells showed association with improved survival in the tumor center. However, the prognostic importance of B cells was not independent, suggesting that they may cooperate with cytotoxic T lymphocytes.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2022-06-22T08:54:04Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-06-22T08:54:04Z (GMT). No. of bitstreams: 0\n Previous issue date: 2022", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "78", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "humoral immunity", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "tumor microenvironment", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "The Prognostic Importance of B Cells and Plasma Cells in Colorectal Cancer", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202206223557", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.collaborator", "value": "public", "language": "", "element": "contractresearch", "qualifier": "collaborator", "schema": "yvv"}, {"key": "yvv.contractresearch.funding", "value": "2000 \u20ac", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "yvv.contractresearch.initiative", "value": "student", "language": "", "element": "contractresearch", "qualifier": "initiative", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solubiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immunohistokemia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "lymfosyytit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell biology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "immunohistochemistry", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "lymphocytes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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