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[{"key": "dc.contributor.advisor", "value": "Penttinen, Reetta", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Jalasvuori, Matti", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Paananen, Petra", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-06-01T11:18:23Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-06-01T11:18:23Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2022", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/81406", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Bakteerien yleistynyt antibioottiresistenssi on vaarantanut antibioottien tehokkuuden bakteeri-infektioiden hoidossa. ESBL:t (Extended-Spectrum Beta- Lactamases) ovat entsyymej\u00e4, jotka antavat kantajabakteerilleen resistenssin useita terveydenhuollossa yleisesti k\u00e4ytettyj\u00e4 beetalaktaamiantibiootteja vastaan. ESBL- geenit ovat useimmiten sijoittuneet liikkuviin plasmideihin, jolloin ne voivat levitt\u00e4\u00e4 resistenssin yhteis\u00f6n muihin bakteereihin. Vaikka osa ihmisist\u00e4 kantaa ESBL-bakteereja suolistossaan oireettomasti, ne aiheuttavat lis\u00e4\u00e4ntyneen komplikaatioriskin tavanomaistenkin l\u00e4\u00e4ketieteellisten toimenpiteiden yhteydess\u00e4. T\u00e4m\u00e4n tutkimuksen tavoitteena oli koostaa ESBL-bakteeria suolistossaan kantavan potilaan suolistomikrobiomista synteettinen bakteeriyhteis\u00f6 ESBL-plasmidien levi\u00e4misen tutkimista varten. Ulosten\u00e4ytteist\u00e4 eristettyj\u00e4 bakteereja karakterisoitiin antibioottiresistenssiprofiilin, 16S rRNA\u2013 ja genomisekvensoinnin avulla. Lopullinen synteettinen yhteis\u00f6 koostui viidest\u00e4 bakteerista, joita olivat Enterococcus faecalis, Citrobacter freundii, Klebsiella sp. ja Comamonas kerstersii sek\u00e4 ESBL-positiivinen Escherichia coli. Yhteis\u00f6n stabiilisuutta tutkittiin seitsem\u00e4n vuorokauden mittaisessa yhteiskasvatuksessa 16S rRNA sekvensoinnin avulla. C. kerstersii -bakteeria lukuun ottamatta kaikki bakteerilajit s\u00e4ilyiv\u00e4t yhteis\u00f6ss\u00e4. Konjugaatiokokeissa havaittiin, ett\u00e4 ESBL-plasmidien siirtymist\u00e4 tapahtui pieniss\u00e4 m\u00e4\u00e4rin E. coli (JM109(pSU19)) bakteerikantaan. Synteettist\u00e4 yhteis\u00f6\u00e4 voidaan tulevaisuudessa k\u00e4ytt\u00e4\u00e4 liikkuvien plasmidien tutkimiseen kontrolloiduissa olosuhteissa ja n\u00e4in ollen paremmin ymm\u00e4rt\u00e4\u00e4 suoliston ESBL-kantajuuden riskej\u00e4.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Antibiotic resistance in bacteria has compromised the effectiveness of antibiotics in treating bacterial infections. Extended-Spectrum Beta-Lactamases (ESBLs) are enzymes that confer multidrug resistance to several beta-lactam antibiotics commonly used in healthcare. ESBL genes are most often located in mobile plasmids, allowing them to spread to other bacteria in a bacterial community. Although some people carry ESBL bacteria in their gut asymptomatically, they may be in increased risk of complications during standard medical procedures. The aim of this study was to assemble a synthetic bacterial community from the intestinal microbiome of a patient carrying ESBL in their gut. This community can be used to study the spread of ESBL plasmids in a controlled system where interspecific bacterial interactions are present. Bacteria were isolated from fecal samples and characterized by their antibiotic resistance profile as well as their 16S rRNA and whole genome sequences. The final synthetic community consisted of five bacteria: Enterococcus faecalis, Citrobacter freundii, Klebsiella sp., Comamonas kerstersii and Escherichia coli, latter of which was ESBL positive. Community stability was studied in a seven-day co-culture by elucidating the community structure by 16S rRNA sequencing. All bacterial species survived in the community, except for C. kerstersii. Conjugation experiments showed that there was a small amount of transfer of ESBL plasmids into the E. coli (JM109 (pSU19)) bacterial strain. In the future, the synthetic community can be used to study mobile plasmids under controlled conditions and thus better understand the risks of intestinal ESBL carriage.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2022-06-01T11:18:23Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-06-01T11:18:23Z (GMT). No. of bitstreams: 0\n Previous issue date: 2022", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "57", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.title", "value": "ESBL-positiivisen suolistobakteeriyhteis\u00f6n karakterisointi ja koostaminen", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202206013030", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "suolisto", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibioottiresistenssi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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