Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding

Cecidomyiidae is a hyperdiverse but inadequately studied dipteran family. The number of described Cecidomyiidae species in the world is 6600 but the true species count is still regarded as unknown. Based on DNA barcodes, the number of Cecidomyiidae species in the world is estimated to be as high as...

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Main Author: Kiljunen, Niina
Other Authors: Matemaattis-luonnontieteellinen tiedekunta, Faculty of Sciences, Bio- ja ympäristötieteiden laitos, Department of Biological and Environmental Science, Jyväskylän yliopisto, University of Jyväskylä
Format: Master's thesis
Language:eng
Published: 2022
Subjects:
Online Access: https://jyx.jyu.fi/handle/123456789/80414
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author Kiljunen, Niina
author2 Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_facet Kiljunen, Niina Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä Kiljunen, Niina Matemaattis-luonnontieteellinen tiedekunta Faculty of Sciences Bio- ja ympäristötieteiden laitos Department of Biological and Environmental Science Jyväskylän yliopisto University of Jyväskylä
author_sort Kiljunen, Niina
datasource_str_mv jyx
description Cecidomyiidae is a hyperdiverse but inadequately studied dipteran family. The number of described Cecidomyiidae species in the world is 6600 but the true species count is still regarded as unknown. Based on DNA barcodes, the number of Cecidomyiidae species in the world is estimated to be as high as 1.8 million. In Finland, no thorough inventories of Cecidomyiidae have been conducted, and the known species count is 384. The purpose of this study was to estimate the expected number of Cecidomyiidae species occurring in Finland utilising BINs (Barcode Index Number) for preliminary species delimitation. Cecidomyiids were collected from eight sites across the country using Malaise traps in the summer of 2020. Supplementary material from 13 sites was also included to the study. The barcode fragment of the COI (cytochrome oxidase c subunit I) gene was sequenced from 9025 Cecidomyiidae specimens. Of the analysed specimens, 7838 recovered validated sequences and were assigned to 1420 BINs. Species count was estimated using model-based extrapolations from rarefaction curves of the abundance data. Whole material is estimated to contain 1.8-fold species count compared to the analysed proportion (⅓). Since further extrapolated rarefaction curve did not reach an asymptote, the estimate of the number of Cecidomyiidae species in Finland still remains largely unknown. However, Finnish Cecidomyiidae fauna evidently consist of thousands of species. The result is congruent with previous studies and points out the efficiency of DNA based methods in dark taxa research.
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The number of described Cecidomyiidae species in the world is 6600 but the true species count is still regarded as unknown. Based on DNA barcodes, the number of Cecidomyiidae species in the world is estimated to be as high as 1.8 million. In Finland, no thorough inventories of Cecidomyiidae have been conducted, and the known species count is 384. The purpose of this study was to estimate the expected number of Cecidomyiidae species occurring in Finland utilising BINs (Barcode Index Number) for preliminary species delimitation. Cecidomyiids were collected from eight sites across the country using Malaise traps in the summer of 2020. Supplementary material from 13 sites was also included to the study. The barcode fragment of the COI (cytochrome oxidase c subunit I) gene was sequenced from 9025 Cecidomyiidae specimens. Of the analysed specimens, 7838 recovered validated sequences and were assigned to 1420 BINs. 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spellingShingle Kiljunen, Niina Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding Barcode Index Number BIN dark taxa gall midges Malaise trap taxonomic impediment Ekologia ja evoluutiobiologia Ecology and evolutionary biology 4011 sääsket kaksisiipiset hyönteiset DNA-viivakoodit biodiversiteetti Nematocera Diptera insects DNA barcodes biodiversity
title Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding
title_full Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding
title_fullStr Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding
title_full_unstemmed Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding
title_short Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding
title_sort estimating cecidomyiidae diptera species richness in finland using dna barcoding
title_txtP Estimating Cecidomyiidae (Diptera) species richness in Finland using DNA barcoding
topic Barcode Index Number BIN dark taxa gall midges Malaise trap taxonomic impediment Ekologia ja evoluutiobiologia Ecology and evolutionary biology 4011 sääsket kaksisiipiset hyönteiset DNA-viivakoodit biodiversiteetti Nematocera Diptera insects DNA barcodes biodiversity
topic_facet 4011 BIN Barcode Index Number DNA barcodes DNA-viivakoodit Diptera Ecology and evolutionary biology Ekologia ja evoluutiobiologia Malaise trap Nematocera biodiversiteetti biodiversity dark taxa gall midges hyönteiset insects kaksisiipiset sääsket taxonomic impediment
url https://jyx.jyu.fi/handle/123456789/80414 http://www.urn.fi/URN:NBN:fi:jyu-202203292099
work_keys_str_mv AT kiljunenniina estimatingcecidomyiidaedipteraspeciesrichnessinfinlandusingdnabarcoding