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[{"key": "dc.contributor.advisor", "value": "Penttinen, Reetta", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Jalasvuori, Matti", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Vacker, Sanna", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Maja, Anni", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Lintil\u00e4, Emilia", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-01-21T06:11:20Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-01-21T06:11:20Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/79444", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Bakteerit ovat kehitt\u00e4neet useita erilaisia mekanismeja, joiden avulla ne voivat\nsuojautua antibiootteja ja muita toksisia yhdisteit\u00e4 vastaan. T\u00e4h\u00e4n tarkoitukseen\ntoimiva keino on vieraan DNA:n hankinta horisontaalisen geeninsiirron avulla, ja\nmy\u00f6s antibioottiresistenssigeenit levi\u00e4v\u00e4t tehokkaasti esimerkiksi konjugatiivisten\nplasmidien mukana. ESBL (engl. extended spectrum beta-lactamase) -bakteerit\nkykenev\u00e4t tuottamaan betalaktamaasi-entsyymej\u00e4, jotka hajottavat useita erilaisia\nbakteeri-infektioiden hoitoon k\u00e4ytettyj\u00e4 betalaktaamiantibiootteja, mink\u00e4 vuoksi ne\novat suuri ongelma terveydenhuollolle. CRICON on kahden plasmidin\nmuodostama, konjugaation avulla liikkuva CRISPR-Cas9 -plasmidisysteemi, joka\nvoidaan ohjelmoida sekvenssispesifisesti katkaisemaan antibioottiresistenssi- kuten\nESBL-geenej\u00e4. Sen on aiemmin tutkittu poistavan tehokkaasti\nbetalaktamaasigeenej\u00e4 bakteeripopulaatioista, kun kohteena k\u00e4ytet\u00e4\u00e4n, Escherichia\ncoli -laboratoriokantoja. Ei kuitenkaan tiedet\u00e4, kuinka hyvin se soveltuu\nantibioottiresistenssigeenien poistamiseen luonnollisista bakteeri-isolaateista.\nT\u00e4m\u00e4n tutkimuksen tarkoituksena olikin selvitt\u00e4\u00e4, kuinka tehokkaasti CRICON\nsiirtyy E. coli -bakteerista kliinisiin ulosten\u00e4ytteist\u00e4 eristettyihin ESBLbakteerikantoihin ja millaiset geneettiset tekij\u00e4t vastaanottajabakteerissa siihen\nvaikuttavat. CRICON-systeemin siirtymist\u00e4 tutkittiin konjugaatiokasvatuksilla,\njoissa CRICON-transkonjugantit selektoitiin antibioottivalinnan avulla. Lopuksi\ntutkittiin my\u00f6s millaiset geneettiset tekij\u00e4t vastaanottavassa ESBL-bakteerissa\nmahdollisesti vaikuttavat CRICON-systeemin levi\u00e4mistehokkuuteen. CRICONsysteemi onnistuttiin siirt\u00e4m\u00e4\u00e4n useaan ESBL-kantaan, joiden joukossa oli usea E.\ncoli -kanta, kaksi Klebsiella pneumoniae -kantaa ja yksi Proteus hauseri -kanta.\nTutkimuksen perusteella n\u00e4ytti my\u00f6s silt\u00e4, ett\u00e4 usean plasmidin esiintyminen\nkohdebakteerissa heikensi CRICON-systeemin siirtymist\u00e4. Tutkimuksen tuloksia\nvoidaan tulevaisuudessa k\u00e4ytt\u00e4\u00e4 CRICON-systeemin kehitt\u00e4miseen ESBLbakteerien poistamiseksi suolistomikrobiomista.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Bacteria have developed multiple different mechanisms to protect themselves\nagainst antibiotics and other toxic compounds. A practical way to do that is by\nacquiring foreign DNA via horizontal gene transfer and antibiotic resistance genes\nalso spread efficiently along with conjugative plasmids. ESBL (extended spectrum\nbeta-lactamase) -bacteria produce beta-lactamases, enzymes that can degrade\nmultiple different beta-lactam antibiotics, and their global prevalence poses a\nsignificant concern to health care. CRICON is a CRISPR-Cas9 plasmid system\ncomposed of two plasmids that move via conjugation. Its CRISPR-system can be\nprogrammed to produce sequence specific DNA nicks to antibiotic resistance genes\nlike ESBL-genes. It has been previously found to eradicate resistance genes\nefficiently from bacterial populations when using Escherichia coli laboratory strains\nas a target. However, it is not known whether it is applicable for removing antibiotic\nresistance genes from natural bacterial isolates. The aim of this study was to study\nthe transfer efficiency of CRICON system from E. coli donor strain to ESBL-recipient\nstrains that were isolated from fecal samples and to examine which genetic traits of\nthe recipient impact on the transfer of the system. Conjugation efficiency of\nCRICON was examined by conjugation cultivations from which the CRICON\ntransconjugants were selected via antibiotic selection. At the end of the study, we\nexamined which genetic traits, such as conjugative plasmids already present in the\nrecipient ESBL-bacteria, affected the CRICON system dispersal. In this study,\nCRICON system was successfully transferred to multiple ESBL strains representing\nspecies such as E. coli, Klebsiella pneumoniae and Proteus hauseri. The study also\nindicated that multiple plasmids carried by the recipient strain had a negative effect\non the CRICON transfer efficiency. In the future, these results can be utilized for\nmore efficient optimization of CRICON system to eradicate ESBL-producing\nbacteria from microbial communities such as gut microbiome.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2022-01-21T06:11:20Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-01-21T06:11:20Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "48", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "CRISPR-Cas9", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "konjugatiivinen plasmidi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "CRICON-plasmidisysteemin siirtyminen kliinisiin ESBL-bakteerikantoihin", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "bachelor thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202201211208", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Bachelor's thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Kandidaatinty\u00f6", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_7a1f", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "bachelorThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibioottiresistenssi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "resistenssi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiootit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geenit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}]
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