| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Jalasvuori, Matti", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Koskinen, Katariina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Thind, Navjot", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2022-01-19T06:51:50Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2022-01-19T06:51:50Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/79417", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Antibioottivastustuskykyisten Klebsiella pneumoniae -bakteerikantojen aiheuttamat infektiot ovat globaalisti kasvava ongelma, sill\u00e4 ne saattavat olla resistenttej\u00e4\nkaikille kliinisess\u00e4 k\u00e4yt\u00f6ss\u00e4 oleville antibiooteille. Infektioiden hoitoon voidaan vaihtoehtoisesti hy\u00f6dynt\u00e4\u00e4 bakteereja tappavia viruksia, bakteriofageja, joita k\u00e4ytet\u00e4\u00e4n infektioiden hoidoissa sellaisenaan tai niiden tuottamien lyyttisten entsyymien kautta. T\u00e4m\u00e4n tutkimuksen tavoitteena on ymm\u00e4rt\u00e4\u00e4 kahden K. pneumoniae -kannan (EKP3 ja EKP8) ja Nen\u00e4inniemen j\u00e4tevedenpuhdistamosta eristettyjen faagien v\u00e4list\u00e4 vuorovaikutusta, sek\u00e4 l\u00f6yt\u00e4\u00e4 uusia n\u00e4k\u00f6kulmia faagiterapian kehitt\u00e4miseksi. EKP3 ja EKP8 villityyppikantojen lis\u00e4ksi molemmista kannoista tutkittavana oli kaksi mutanttia isolaattia, jotka olivat kehittyneet resistenteiksi kahdeksalle ennest\u00e4\u00e4n eristetyille faageille. Kannat erosivat toisistaan geneettisesti siten, ett\u00e4 ainoastaan toisella kannoista (EKP3:lla) on CRISPR-lokuksia. \nTutkimuksen aikana suoritetut faagialtistukset paljastivat EKP3-kannan reagoivan faageihin geneettisell\u00e4 tasolla, toisin kuin EKP8, joka reagoi voimakkaasti muokkaamalla geenis\u00e4\u00e4tely\u00e4. Uusien, faagiterapian kannalta, potentiaalisten faagien erist\u00e4minen j\u00e4tevedest\u00e4 onnistui hyvin. Erityisen kiinnostavaa on EKP8P6-\nfaagin kyky infektoida EKP3-kantaa vain silloin, kun se ensin oli kehitt\u00e4nyt resistenssin EKP8P3-faagia vastaan. Vastaavia resistenttej\u00e4 muotoja infektoivia faageja altistuksiin lis\u00e4\u00e4m\u00e4ll\u00e4 voidaan mahdollisesti parantaa faagiterapian onnistumistodenn\u00e4k\u00f6isyytt\u00e4 ja hoidon tehokkuutta tulevaisuudessa. Faagialtistuskokeet eiv\u00e4t laboratorio-oloissa aktivoineet is\u00e4nn\u00e4n CRISPR-j\u00e4rjestelm\u00e4\u00e4, mit\u00e4 voidaan pit\u00e4\u00e4 my\u00f6s lupaavana faagiterapian kannalta.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Antibiotics have been used for decades to treat bacterial infections, such as ones caused by Klebsiella pneumoniae, a well-known multi-drug resistant pathogen. The efficacy of antibiotics in the treatment of bacterial infections has, however, been \nweakened by the growing prevalence of antibiotic resistance genes in the bacterial population. Bacteriophages, or phages for short, are viruses which specifically infect bacteria and therefore could act as an alternative for antibiotic treatments in the form of phage therapy. The purpose of this study was to examine the responses of two K. pneumoniae strains (EKP3 and EKP8) to isolated phages to further improve our knowledge on phage therapy. Both strains had two mutant isolates that were previously treated with eight phages and had developed resistances against them. These strains differed from each other genetically by CRISPR-loci that only EKP3 possessed, though it wasn\u00b4t activated during any of the experiments. Results showed how EKP3 altered its genome to adapt to environmental changes and phage pressure, unlike EKP8, which altered its gene expression instead. We were able to isolate promising novel phages from sewage water, especially EKP8P6, which was able to infect EKP3 only when the bacterium had developed resistance against\nanother phage isolated from sewage previously. This study shows new insights into how we could insert phages like EKP8P6 that cannot infect wild-type bacteria but rather mutated ones, into phage cocktails to improve phage therapy treatments against pathogenic bacteria like K. pneumoniae.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2022-01-19T06:51:50Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2022-01-19T06:51:50Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "84", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "fin", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "CRISPR", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "infektiokoe", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Klebsiella pneumoniae -bakteerilajia infektoivien faagien eristys ja faagiresistenssimekanismien kartoittaminen", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202201191185", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "fagiterapia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mutaatiot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "resistenssi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteriofagit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "infektiot", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiootit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "DNA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "taudinaiheuttajat", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "Klebsiella-bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|