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[{"key": "dc.contributor.advisor", "value": "Permi, Perttu", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Aitio, Helena", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Pitk\u00e4nen, Ilona", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2021-10-06T04:58:44Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2021-10-06T04:58:44Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/78031", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Yleistyv\u00e4 antibioottiresistenssi on yksi t\u00e4m\u00e4n hetken suurimmista terveydenhuollon tulevaisuutta uhkaavista haasteista. Erityisen huolestuttava ilmi\u00f6 on moniresistenttien bakteerikantojen, kuten metisilliinille resistentin Staphylococcus aureuksen eli MRSA:n, yleistyminen. Resistentteihin kantoihin liittyy usein muita kantoja korkeampi kuolleisuus sek\u00e4 taloudellisesti suuremmat kustannukset, mik\u00e4 korostaa tarvetta kehitt\u00e4\u00e4 vaihtoehtoisia tapoja hoitaa bakteeri-infektioita. Er\u00e4s kiinnostavista vaihtoehdoista on bakteerien solusein\u00e4n peptidoglykaania hajottava entsyymiryhm\u00e4 peptidoglykaanihydrolaasit. T\u00e4ss\u00e4 tutkimuksessa k\u00e4siteltiin yht\u00e4 n\u00e4ist\u00e4 entsyymeist\u00e4, lysostafiineihin kuuluvaa S. aureuksen autolysiini\u00e4 LytM. T\u00e4m\u00e4n tutkimuksen tavoitteena oli karakterisoida LytM:n N-terminaalisen domeenin liuosrakenne NMR-spektroskopiaa hy\u00f6dynt\u00e4en ja selvitt\u00e4\u00e4 aiemmasta kiderakenteesta puuttuvien ep\u00e4j\u00e4rjest\u00e4ytyneiden alueiden rakenne. Rakenteen m\u00e4\u00e4ritt\u00e4misen lis\u00e4ksi LytM:n kahden domeenin v\u00e4lisi\u00e4 vuorovaikutuksia sek\u00e4 niit\u00e4 yhdist\u00e4v\u00e4n linkkerialueen merkityst\u00e4 tutkittiin titraatiokokeiden avulla. Tuloksena N-terminaaliselle domeenille m\u00e4\u00e4ritettiin laadultaan hyv\u00e4 liuosrakenne, josta havaittiin konstruktin N- ja C-terminaalisten p\u00e4iden olevan ep\u00e4j\u00e4rjest\u00e4ytyneit\u00e4. Titraatiokokeet osoittivat, ett\u00e4 domeenien v\u00e4lisen vuorovaikutuksen muodostumiseen tarvitaan v\u00e4hint\u00e4\u00e4n osittainen linkkerialue. Linkkerin todettiin laskostuvan osittain vuorovaikutuksen yhteydess\u00e4, ja vuorovaikutuspinta m\u00e4\u00e4ritettiin proteiinin sekvenssiss\u00e4 aminohappoihin 130-148. Lis\u00e4tutkimuksia tarvitaan aktivaatiomekanismin sek\u00e4 N-terminaalisen domeenin merkityksen ja toimintojen selvitt\u00e4miseksi.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "The emergence of antibiotic resistance is one of the greatest challenges the field of healthcare is currently facing. The increasing prevalence of multidrug-resistant strains such as MRSA, the methicillin-resistant Staphylococcus aureus, is particularly alarming, as these strains are associated with higher costs and mortality. Thus, the development of alternative solutions for treating bacterial infections is a pressing issue. One of the potential alternatives to antibiotic drugs is a group of bacterial cell wall-degrading enzymes known as peptidoglycan hydrolases. One of these enzymes, LytM, an S. aureus autolysin of the lysostaphin family, was studied in this project. The aim was to characterize the solution structure of LytM N-terminal domain using NMR spectroscopy, with the focus on determining the putative disordered regions. Additionally, the interaction of the two domains of LytM was studied by titration experiments to better understand the binding of the domains and the role of the linker region connecting them. As a result, a solution structure of good quality was obtained, confirming the disordered nature of the N- and C-terminal ends of the construct. The interaction study showed that either a partial or complete linker was required for the two domains to interact. It was deduced that partial folding of the C-terminal end of the linker takes place when the domains interact, and the interaction surface was located to residues 130-148 in the amino acid sequence of the protein. Based on the findings, it was concluded that the linker region is essential for the interaction. Further studies are required for elucidating the activation mechanisms of LytM and the function of the N-terminal domain.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2021-10-06T04:58:44Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2021-10-06T04:58:44Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "49", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "NMR spectroscopy", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "autolysins", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "lysostaphins", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "peptidoglycan hydrolases", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Biophysical characterization and interaction study of Staphylococcus aureus LytM", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202110065082", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiootit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibioottiresistenssi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "vuorovaikutus", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "stafylokokit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solubiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "molekyylibiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "MRSA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "solusein\u00e4t", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiotics", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "antibiotic resistance", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "interaction", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "staphylococci", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell biology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "molecular biology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "MRSA", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cell walls", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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