| fullrecord |
[{"key": "dc.contributor.advisor", "value": "Knott, Emily", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Hiillos, Anna-Lotta", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Kolehmainen, Aleksi", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2021-07-02T09:18:12Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2021-07-02T09:18:12Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/76967", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Mikrobipopulaatiot ovat olennainen osa jokaista ekosysteemi\u00e4. T\u00e4st\u00e4 huolimatta alkueli\u00f6t ovat kuitenkin huomattavasti v\u00e4hemm\u00e4n tutkittuja prokaryootteihin verrattuna. T\u00e4m\u00e4 maisteritutkielma tutkii mikrobipopulaatioiden diversiteetti\u00e4 It\u00e4meress\u00e4 uuden sukupolven sekvensointimenetelmill\u00e4. Tutkimus keskittyy erityisesti alkuel\u00e4inpopulaatioihin. P\u00e4\u00e4m\u00e4\u00e4r\u00e4n\u00e4 on selvitt\u00e4\u00e4, kuinka It\u00e4meren suolagradientti ja ajallinen vaihtelu vaikuttavat populaatioiden rakenteisiin. Hypoteesina on, ett\u00e4 bakteeripopulaatioiden diversiteetti pysyisi tasaisena ajasta ja paikasta riippumatta, mutta alkueli\u00f6iden m\u00e4\u00e4r\u00e4t vaihtelisivat enemm\u00e4n. Ty\u00f6ss\u00e4 k\u00e4ytetyt n\u00e4ytteet ker\u00e4ttiin kolmelta eri n\u00e4ytteenottopaikalta nelj\u00e4sti vuodessa. N\u00e4ytteet sekvensoitiin ja tulokset analysoitiin RStudio-ohjelmalla. Bakteereissa havaittiin merkitt\u00e4v\u00e4\u00e4 vaihtelua ajallisesti. Alkueli\u00f6iden diversiteetti vaihteli paikallisesti. Dinoflagellata oli suhteellisesti yleisin p\u00e4\u00e4jakso elokuussa 2018 l\u00e4hes kaikilla n\u00e4ytteenottopaikoilla, mutta m\u00e4\u00e4r\u00e4 laski vuoden edetess\u00e4. Ochrophyta puolestaan kasvoi m\u00e4\u00e4r\u00e4llisesti vuoden aikana. Syy t\u00e4h\u00e4n ilmi\u00f6\u00f6n oli todenn\u00e4k\u00f6isesti vuoden 2018 elokuun korkea l\u00e4mp\u00f6tila. Tulokset eiv\u00e4t noudattaneet hypoteesia, jonka mukaan alkueli\u00f6t olisivat alttiimpia ymp\u00e4rist\u00f6tekij\u00f6ille, sill\u00e4 bakteereiden diversiteetin vaihtelu ajallisesti oli vastoin odotuksia.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Microbe populations are essential for every ecosystem and have various functions. However, protists are notably less studied compared to prokaryotic microbes. This master\u2019s thesis focuses on studying the diversity of microbe populations in the Baltic Sea using next-generation sequencing. The focus is especially on the protist diversity. The aim is to find out whether the salinity gradient and seasonal changes have an effect on the structure of the microbe population. It was hypothesized that the bacterial diversity would remain steady throughout the year regardless of surrounding conditions, while protists would experience more variation. The samples used in the thesis were taken from three different sites with varying salinity in the Baltic Sea. The sampling was repeated four times throughout the year. The samples were sequenced and the results were statistically analyzed with RStudio. Bacteria showed significant temporal variation in diversity, with Cyanobacteria and Proteobacteria being the most common phyla. Protist populations varied spatially. More variation could be seen on the class level. Dinoflagellata were the most abundant phylum in nearly all sites in August 2018, but declined throughout the year, while the abundance of Ochrophyta grew. This was most likely due to exceptionally high temperatures during August 2018.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2021-07-02T09:18:12Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2021-07-02T09:18:12Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "67", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "Sequencing", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Prokaryotes", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "Alpha Diversity", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "16S rDNA", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "18S rDNA", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Temporal and Spatial Variation in Microbial Communities of Aquatic Sediments in the Baltic Sea", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202107024154", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "alkueli\u00f6t", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "aitotumaiset", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bakteerit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikrobiologia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "mikro-organismit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "iti\u00f6el\u00e4imet", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "protists", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "eukaryote", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microbes", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bacteria", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microbiology", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "microorganisms", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "Apicomplexa", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
|