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[{"key": "dc.contributor.advisor", "value": "Yl\u00e4nne, Jari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Pekkanen-Mattila, Mari", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Pirhonen, Jonatan", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2021-07-02T09:12:14Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2021-07-02T09:12:14Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/76966", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Hapenpuutteesta johtuvat syd\u00e4nsairaudet (iskeemiset syd\u00e4nsairaudet) ovat maailmanlaajuinen ongelma kuolevuuden kannalta, joista syd\u00e4ninfarkti on kaikista yleisin. T\u00e4ll\u00e4 hetkell\u00e4 ei viel\u00e4 ole paljon k\u00e4ytett\u00e4viss\u00e4 olevia menetelmi\u00e4, joiden avulla voitaisiin parantaa potilaiden iskeemist\u00e4 syd\u00e4nkudosta. Syd\u00e4nkudos voidaan hapettaa uudelleen, mutta t\u00e4m\u00e4n on silti havaittu aiheuttavan lis\u00e4vahinkoa syd\u00e4men toiminnassa reperfuusiovaurion muodossa ja siksi uusien terapeuttisten menetelmien kehitt\u00e4minen iskeemisen kudoksen pelastamiseksi on t\u00e4rke\u00e4\u00e4. Syd\u00e4ninfarktissa verenkiertoa rajoittaa sepelvaltimotukos, joka est\u00e4\u00e4 hapen virtauksen sek\u00e4 ravinteiden kulkeutumisen syd\u00e4nlihakseen. T\u00e4m\u00e4 rajoitus aiheuttaa edelleen matalan happitilan eli hypoksian syd\u00e4nlihassoluissa, mik\u00e4 puolestaan johtaa syd\u00e4nlihassolujen vialliseen supistumiseen, elektrofysiologisiin h\u00e4iri\u00f6ihin ja lopulta solukuolemaan. Koska syd\u00e4ninfarktin patofysiologisia mekanismeja ei viel\u00e4 tunneta yksityiskohtaisesti, ihmisper\u00e4isten in vitro -solumallien tarve hapen puutteen vaikutusten tutkimiseen syd\u00e4nkudoksessa on lis\u00e4\u00e4ntynyt. T\u00e4ss\u00e4 tutkimuksessa pyrittiin m\u00e4\u00e4ritt\u00e4m\u00e4\u00e4n hypoksian vaikutuksia ihmisen indusoiduista pluripotenteista kantasoluista (hiPSC) erilaistettujen syd\u00e4nlihassolujen sykenopeuteen. Mekaanista sykkimisaktiivisuutta tutkittiin modulaarisella alustalla, joka mahdollistaa samanaikaisen videomikroskopian, elektrofysiologian r\u00e4\u00e4t\u00e4l\u00f6idyn mikroelektrodij\u00e4rjestelm\u00e4n avulla ja hapen mittaamisen fluoresoivalla laserilla kontrolloiduissa viljelyolosuhteissa. Alkiokantasoluker\u00e4ym\u00e4tekniikalla erilaistetut syd\u00e4nlihassolurypp\u00e4\u00e4t altistettiin pitkittyneelle 8 tunnin hypoksiajaksolle ja hapetettiin sen j\u00e4lkeen uudelleen. Tulosten mukaan hapen puute syd\u00e4nlihassoluissa muuttaa sykkimisaktiivisuutta v\u00e4hent\u00e4m\u00e4ll\u00e4 sykkeen m\u00e4\u00e4r\u00e4\u00e4 huomattavasti. Hapen osapaineen ja sykenopeuden v\u00e4linen lineaarinen korrelaatioanalyysi n\u00e4ytti my\u00f6s, ett\u00e4 sykenopeuden muutos on suoraan verrannollinen happipitoisuuden v\u00e4henemiseen. Lis\u00e4ksi, koska happea havaitseva materiaali on todetusti myrkyt\u00f6n soluille, syd\u00e4nlihassolujen toiminnallisia ominaisuuksia voidaan arvioida turvallisesti ja tarkasti kyseisill\u00e4 menetelmill\u00e4.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Ischemic heart disease is one of the major reasons of deaths and morbidity in the world, myocardial infarction being the most common one. Nowadays, there are not much available methods to rescue the ischemic tissue in patients. The ischemic tissue can be reoxygenated, but still this has been found out to cause further damage on the cardiac tissue in the form of reperfusion-injury. Therefore, the development of new therapeutic approaches concerning this matter are urgent. In myocardial infarction the coronary blood flow is restricted by occlusion, thus preventing oxygen flow as well as nutrient delivery to the myocardium. This restriction further causes a low oxygen state called hypoxia inside cardiomyocytes, which in turn leads to failures in cardiomyocyte contraction, disturbances in electrophysiology and eventually cell death. As the pathophysiological mechanisms of myocardial infarction are not known in detail, there is a need of human based in vitro models to clarify more the effects of oxygen deprivation in the cardiac tissue. Here, the study aimed to determine effects of hypoxia on the beating rate of human induced pluripotent stem cell (hiPSC) derived cardiomyocytes. The mechanical beating activity was studied with a modular platform that enables simultaneous video microscopy, electrophysiology via a custom-made micro electrode array and fluorescent oxygen measuring in controlled culture conditions. Embryoid body differentiated cardiomyocyte clusters were exposed to a prolonged hypoxia period of 8 h and reoxygenated after that. According to the results, oxygen deprivation in cardiomyocytes alters the beating activity by decreasing the beating rate significantly. A linear correlation analysis between oxygen partial pressure and the beating rate also suggested that the change in beating rate is directly proportional with the decrease in oxygen concentration. In addition, as the oxygen sensing material was found to be non-toxic for the cells, the functional characteristics of cardiomyocytes can be safely and precisely assessed with these methods.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2021-07-02T09:12:14Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2021-07-02T09:12:14Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "54", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "hiPSC", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "cardiomyocyte", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "embryoid body", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "video microscopy", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "micro electrode array", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "oxygen measuring", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Beating rate analysis of hiPSC derived cardiomyocytes for ischemia disease modeling", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202107024153", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "yvv.contractresearch.initiative", "value": "university", "language": "", "element": "contractresearch", "qualifier": "initiative", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "kantasolut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "syd\u00e4nlihassolut", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hypoksia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "iskemia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "stem cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "cardiac muscle cells", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "hypoxia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "ischemia", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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