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[{"key": "dc.contributor.advisor", "value": "Vesterinen, Tiina", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Arola, Johanna", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Kuopio, Teijo", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Niinim\u00e4ki, Jenni", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2021-07-01T05:18:49Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2021-07-01T05:18:49Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/76923", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Sy\u00f6v\u00e4n kehittymisess\u00e4 tietyt geenit ovat usein yliekspressoituja ja niiden tunnistaminen tarjoaa mahdollisuuksia l\u00f6yt\u00e4\u00e4 diagnostisia, prediktiivisi\u00e4 ja prognostisia merkkiaineita sek\u00e4 kohteita sy\u00f6p\u00e4tautien l\u00e4\u00e4kehoidoille. T\u00e4ss\u00e4 tutkimuksessa RNA-sekvensoinnin ja immunohistokemian avulla pyrittiin tunnistamaan uusia merkkiaineita keuhkon karsinoidikasvaimista. Tutkimusaineisto koostui 128:n Helsingin yliopistollisen sairaalan karsinoidikasvainpotilaan (PC) diagnostisista formaliinifiksoiduista, parafiiniin valetuista kasvainn\u00e4ytteist\u00e4 vuosilta 1990\u20132013. Geeniekspressiota tutkittaessa HSP90AB1 osoitti voimakasta ilmentymist\u00e4 metastasoineiden PC-kasvainten ryhm\u00e4ss\u00e4 (p = 0.002). My\u00f6s geenin paralogi, HSP90AA1, ekspressoitui normaalia voimakkaammin. Arvioitaessa immunohistokemiallisin v\u00e4rj\u00e4yksin n\u00e4iden geenien koodaamien proteiinien ekspressiotasojen suhdetta erilaisiin kliinispatologisiin muuttujiin, havaittiin korkean Hsp90-\u03b1 ja -\u03b2 ekspression assosioituvan potilaan huonontuneeseen ennusteeseen (p = 0.009) sek\u00e4 kohonneeseen tautikuoleman riskiin. Johtop\u00e4\u00e4t\u00f6ksen\u00e4, RNA-sekvensointi osoittautui toimivan hyvin geenien ilmentymisen tutkimiseen formaliinifiksoiduista, parafiiniin valetuista PC-kasvainn\u00e4ytteist\u00e4. Menetelm\u00e4\u00e4 hy\u00f6dynt\u00e4en kyettiin osoittamaan yhteys kohonneen HSP90AB1-ekspression ja kasvaimen aggressiivisuuden v\u00e4lill\u00e4. Immunohistokemialla osoitettavaa sytoplasmista Hsp90-\u03b1 ja -\u03b2 ilmentymist\u00e4 voitaneen jatkossa hy\u00f6dynt\u00e4\u00e4 yksitt\u00e4isten PC-potilaiden ennusteen arvioinnissa. Hsp90-proteiinin inhibointi voisi olla tulevaisuuden hoitomuoto PC-tautiin.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "In cancer initiation, development and progress some genes are often overexpressed or altered, providing potential diagnostic, predictive and prognostic markers as well as therapeutic targets for the treatment. In this study, RNA sequencing and immunohistochemistry were used to detect novel biomarkers for pulmonary carcinoid (PC) tumor patients. Altogether, 128 formalin-fixed, paraffin-embedded PC tumor samples from patients who were surgically treated in the Helsinki University Hospital between 1990 and 2013 were included in the study. In gene expression analysis, HSP90AB1 mRNA expression was found to be significantly elevated in the group of metastatic PC tumors (p = 0.002). The paralog of the gene, HSP90AA1, was also somewhat overexpressed. When immunohistochemistry was used to assess the relationship between the expression levels of the proteins encoded by these genes and various clinicopathological factors, increased Hsp90-\u03b1 and -\u03b2 expression was found to be associated with poor patient outcome (p = 0.009) and increased risk of disease-specific death. In conclusion, RNA sequencing was found to serve well in gene expression analyzes of formalin-fixed, paraffin-embedded PC tumor samples and the method was able to demonstrate an association between the overexpression of HSP90AB1 and the aggressiveness of the tumor. The immunohistochemical assessment of cytoplasmic Hsp90-\u03b1 and -\u03b2 protein expressions could be further utilized to assess the prognosis of individual patients. Based on these findings, inhibition of Hsp90 could become potential treatment target for PC disease.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Miia Hakanen (mihakane@jyu.fi) on 2021-07-01T05:18:49Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2021-07-01T05:18:49Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "83", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "atyyppinen karsinoidi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "tyypillinen karsinoidi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "l\u00e4mp\u00f6shokkiproteiini", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "transkriptomi", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "atypical carcinoid", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "typical carcinoid", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "heat-shock protein", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "transcriptome", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "RNA sequencing and immunohistochemistry as tools to identify new biomarkers for pulmonary carcinoid tumor patients", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202107014111", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Bio- ja ymp\u00e4rist\u00f6tieteiden laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Biological and Environmental Science", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Solu- ja molekyylibiologia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Cell and molecular biology", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "restrictedAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4013", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "geeniekspressio", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bioinformatiikka", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "karsinoidit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "gene expression", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "bioinformatics", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "carcinoids", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "The author has not given permission to make the work publicly available electronically. Therefore the material can be read only at the archival workstation at Jyv\u00e4skyl\u00e4 University Library (https://kirjasto.jyu.fi/collections/archival-workstation).", "language": "en", "element": "rights", "qualifier": "accessrights", "schema": "dc"}, {"key": "dc.rights.accessrights", "value": "Tekij\u00e4 ei ole antanut lupaa avoimeen julkaisuun, joten aineisto on luettavissa vain Jyv\u00e4skyl\u00e4n yliopiston kirjaston arkistoty\u00f6semalta. Ks. https://kirjasto.jyu.fi/kokoelmat/arkistotyoasema..", "language": "fi", "element": "rights", "qualifier": "accessrights", "schema": "dc"}]
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