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[{"key": "dc.contributor.advisor", "value": "Nissinen, Maija", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.advisor", "value": "Schirmer, Johanna", "language": "", "element": "contributor", "qualifier": "advisor", "schema": "dc"}, {"key": "dc.contributor.author", "value": "Korhonen, Elsa", "language": "", "element": "contributor", "qualifier": "author", "schema": "dc"}, {"key": "dc.date.accessioned", "value": "2021-06-17T09:34:47Z", "language": null, "element": "date", "qualifier": "accessioned", "schema": "dc"}, {"key": "dc.date.available", "value": "2021-06-17T09:34:47Z", "language": null, "element": "date", "qualifier": "available", "schema": "dc"}, {"key": "dc.date.issued", "value": "2021", "language": "", "element": "date", "qualifier": "issued", "schema": "dc"}, {"key": "dc.identifier.uri", "value": "https://jyx.jyu.fi/handle/123456789/76634", "language": null, "element": "identifier", "qualifier": "uri", "schema": "dc"}, {"key": "dc.description.abstract", "value": "Pro gradu -tutkielman kirjallisuusosassa perehdyttiin menetelmiin, jotka voisivat soveltua proteiinien kiinnitt\u00e4miseen grafeenioksidin pintaan kovalenttisen sitoutumisen kautta. Kokeellisessa osassa oli tavoitteena kiinnitt\u00e4\u00e4 linkkerimolekyylien avulla piparjuuriperoksidaasi (HRP) -entsyymimolekyylej\u00e4 laserilla hapetetun grafeenin pintaan. Alustavissa kokeissa testattiin kahta menetelm\u00e4\u00e4 HRP:n kiinnitt\u00e4miseksi grafeenioksidihiutaleisiin. Menetelm\u00e4t erosivat k\u00e4ytettyjen linkkerimolekyylien suhteen. Ensimm\u00e4isess\u00e4 menetelm\u00e4ss\u00e4 k\u00e4ytettiin glutaraldehydi\u00e4 ja toisessa APTES:sta ja glutaraldehydist\u00e4 muodostuvaa linkkeri\u00e4. Termogravimetrinen analyysi ja FTIR-mittausten tulokset viittasivat kovalenttisten sidosten muodostumiseen grafeenioksidin ja linkkerien sek\u00e4 linkkerien ja HRP:n v\u00e4lille. Glutaraldehydiin perustuva menetelm\u00e4 valittiin varsinaiseen kokeeseen, jossa k\u00e4ytettiin laserilla k\u00e4sitelty\u00e4 grafeenipohjaista mikrosirua. Mikrosirusta otetuista AFM-kuvista havaittiin kohonneita viivamaisia ja pistem\u00e4isi\u00e4 rakenteita funktionalisoinnin j\u00e4lkeen, joiden p\u00e4\u00e4teltiin olevan glutaraldehydiketjuja ja HRP-proteiinimolekyylej\u00e4. Mikrosirun hapetetuista alueista mitatut Raman-spketrit viittasivat muutoksiin hapetetun grafeenin elektronirakenteessa: D-, G- ja 2D-v\u00f6iden Raman-siirtym\u00e4t pieneneniv\u00e4t ja D-vy\u00f6n pinta-ala kasvoi funktionalisoinnin j\u00e4lkeen. Muutosten p\u00e4\u00e4teltiin johtuvan grafeenioksidin ja linkkerien tai HRP:n v\u00e4lisist\u00e4 vuorovaikutuksista. Tulosten perusteella ei kuitenkaan voitu varmistaa HRP:n kovalenttista kiinnittymist\u00e4 mikrosirun grafeenioksidipintaan glutaraldehydin v\u00e4lityksell\u00e4.", "language": "fi", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.abstract", "value": "The literature part of the master\u2019s thesis focuses on introducing potential functionalization methods for the covalent attachment of proteins on graphene oxide (GO) surfaces. The experimental part aimed at the covalent immobilization of horseradish peroxidase (HRP) on the laser-oxidized graphene-based microchip. Based on the preliminary studies with graphene oxide flakes, both crosslinker systems, glutaraldehyde (GA) and APTES-glutaraldehyde, resulted in a similar outcome of the HRP immobilization. The thermogravimetric analysis and FTIR spectroscopic results suggested the formation of covalent bonds between the components of the GO-crosslinker-HRP systems.\nThe GA-based crosslinking method was chosen for the protein immobilization studies with the graphene-based microchip. From the atomic force microscopy (AFM) images of the microchip after the treatment with GA and HRP solutions (in PBS buffer), line-shaped structures and elevated dots could be observed, assigned to be GA crosslinkers and HRP protein molecules, respectively. The Raman spectra of the oxidized areas showed shifting of the D, G, and 2D bands towards lower Raman shifts after HRP immobilization, which agree with the former results, indicating a successful immobilization of HRP. Also, the D band areas of the oxidized regions increased after HRP immobilization, suggesting an increased number of defects in the graphene lattice. Based on the AFM and Raman spectroscopy results from the experiments with the chip, the covalent attachment of HRP to the chip\u2019s surface via GA crosslinker could not be fully proved.", "language": "en", "element": "description", "qualifier": "abstract", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Submitted by Paivi Vuorio (paelvuor@jyu.fi) on 2021-06-17T09:34:47Z\nNo. of bitstreams: 0", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.description.provenance", "value": "Made available in DSpace on 2021-06-17T09:34:47Z (GMT). No. of bitstreams: 0\n Previous issue date: 2021", "language": "en", "element": "description", "qualifier": "provenance", "schema": "dc"}, {"key": "dc.format.extent", "value": "109", "language": "", "element": "format", "qualifier": "extent", "schema": "dc"}, {"key": "dc.format.mimetype", "value": "application/pdf", "language": null, "element": "format", "qualifier": "mimetype", "schema": "dc"}, {"key": "dc.language.iso", "value": "eng", "language": null, "element": "language", "qualifier": "iso", "schema": "dc"}, {"key": "dc.rights", "value": "In Copyright", "language": "en", "element": "rights", "qualifier": null, "schema": "dc"}, {"key": "dc.subject.other", "value": "functionalization", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "protein immobilization", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "covalent interactions", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.subject.other", "value": "graphene-based surfaces", "language": "", "element": "subject", "qualifier": "other", "schema": "dc"}, {"key": "dc.title", "value": "Covalent functionalization of graphene oxide with proteins", "language": "", "element": "title", "qualifier": null, "schema": "dc"}, {"key": "dc.type", "value": "master thesis", "language": null, "element": "type", "qualifier": null, "schema": "dc"}, {"key": "dc.identifier.urn", "value": "URN:NBN:fi:jyu-202106173832", "language": "", "element": "identifier", "qualifier": "urn", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Pro gradu -tutkielma", "language": "fi", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.type.ontasot", "value": "Master\u2019s thesis", "language": "en", "element": "type", "qualifier": "ontasot", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Matemaattis-luonnontieteellinen tiedekunta", "language": "fi", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.faculty", "value": "Faculty of Sciences", "language": "en", "element": "contributor", "qualifier": "faculty", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Kemian laitos", "language": "fi", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.department", "value": "Department of Chemistry", "language": "en", "element": "contributor", "qualifier": "department", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "Jyv\u00e4skyl\u00e4n yliopisto", "language": "fi", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.contributor.organization", "value": "University of Jyv\u00e4skyl\u00e4", "language": "en", "element": "contributor", "qualifier": "organization", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Orgaaninen kemia", "language": "fi", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "dc.subject.discipline", "value": "Organic Chemistry", "language": "en", "element": "subject", "qualifier": "discipline", "schema": "dc"}, {"key": "yvv.contractresearch.funding", "value": "0", "language": "", "element": "contractresearch", "qualifier": "funding", "schema": "yvv"}, {"key": "dc.type.coar", "value": "http://purl.org/coar/resource_type/c_bdcc", "language": null, "element": "type", "qualifier": "coar", "schema": "dc"}, {"key": "dc.rights.accesslevel", "value": "openAccess", "language": null, "element": "rights", "qualifier": "accesslevel", "schema": "dc"}, {"key": "dc.type.publication", "value": "masterThesis", "language": null, "element": "type", "qualifier": "publication", "schema": "dc"}, {"key": "dc.subject.oppiainekoodi", "value": "4035", "language": "", "element": "subject", "qualifier": "oppiainekoodi", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteiinit", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "grafeenioksidi", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "proteins", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.subject.yso", "value": "graphene oxide", "language": null, "element": "subject", "qualifier": "yso", "schema": "dc"}, {"key": "dc.format.content", "value": "fulltext", "language": null, "element": "format", "qualifier": "content", "schema": "dc"}, {"key": "dc.rights.url", "value": "https://rightsstatements.org/page/InC/1.0/", "language": null, "element": "rights", "qualifier": "url", "schema": "dc"}, {"key": "dc.type.okm", "value": "G2", "language": null, "element": "type", "qualifier": "okm", "schema": "dc"}]
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